Arabinoxylan degradation by fungi: characterization of the arabinoxylan-arabinofuranohydrolase encoding genes from Aspergillus niger and Aspergillus tubingensis

1997 ◽  
Vol 31 (1) ◽  
pp. 22-29 ◽  
Author(s):  
Marco M. C. Gielkens ◽  
Jaap Visser ◽  
L. H. de Graaff
Keyword(s):  
Aspergillus Niger ◽  
Aspergillus Tubingensis ◽  
Arabinoxylan Arabinofuranohydrolase ◽  
Encoding Genes

Characterization of the Aspergillus niger prtT, a unique regulator of extracellular protease encoding genes

2008 ◽  
Vol 45 (12) ◽  
pp. 1591-1599 ◽  
Author(s):  
Peter J. Punt ◽  
Frank H.J. Schuren ◽  
Jan Lehmbeck ◽  
Tove Christensen ◽  
Carsten Hjort ◽  
...  
Keyword(s):  
Aspergillus Niger ◽  
Extracellular Protease ◽  
Encoding Genes

Isolation and characterization of two specific regulatory Aspergillus niger mutants shows antagonistic regulation of arabinan and xylan metabolism

Microbiology ◽  
2003 ◽  
Vol 149 (5) ◽  
pp. 1183-1191 ◽  
Author(s):  
Marco J. L. de Groot ◽  
Peter J. I. van de Vondervoort ◽  
Ronald P. de Vries ◽  
Patricia A. vanKuyk ◽  
George J. G. Ruijter ◽  
...  
Keyword(s):  
Aspergillus Niger ◽  
Regulatory System ◽  
Gene Encoding ◽  
Isolation And Characterization ◽  
Catabolic Genes ◽  
Genes Encoding ◽  
Intracellular Activities ◽  
Xylulose Kinase ◽  
Encoding Genes

This paper describes two Aspergillus niger mutants (araA and araB) specifically disturbed in the regulation of the arabinanase system in response to the presence of l-arabinose. Expression of the three known l-arabinose-induced arabinanolytic genes, abfA, abfB and abnA, was substantially decreased or absent in the araA and araB strains compared to the wild-type when incubated in the presence of l-arabinose or l-arabitol. In addition, the intracellular activities of l-arabitol dehydrogenase and l-arabinose reductase, involved in l-arabinose catabolism, were decreased in the araA and araB strains. Finally, the data show that the gene encoding d-xylulose kinase, xkiA, is also under control of the arabinanolytic regulatory system. l-Arabitol, most likely the true inducer of the arabinanolytic and l-arabinose catabolic genes, accumulated to a high intracellular concentration in the araA and araB mutants. This indicates that the decrease of expression of the arabinanolytic genes was not due to lack of inducer accumulation. Therefore, it is proposed that the araA and araB mutations are localized in positive-acting components of the regulatory system involved in the expression of the arabinanase-encoding genes and the genes encoding the l-arabinose catabolic pathway.

Extraction and Characterization of Antibacterial Compound from Aspergillus niger

2013 ◽  
Vol 16 (4) ◽  
pp. 167-174 ◽  
Author(s):  
Abdulwahid B. A. Al-Shaibani ◽  
◽  
Faiz I. Al-Shakarchi ◽  
Rasha S. Ameen ◽  
◽  
...  
Keyword(s):  
Aspergillus Niger ◽  
Antibacterial Compound

scholarly journals Purification and characterization of endoxylanase Xln-1 from Aspergillus niger B03

2009 ◽  
Vol 25 (12) ◽  
pp. 2095-2102 ◽  
Author(s):  
Georgi Dobrev ◽  
Boriana Zhekova ◽  
Ginka Delcheva ◽  
Lidia Koleva ◽  
Nicola Tziporkov ◽  
...  
Keyword(s):  
Aspergillus Niger ◽  
Purification And Characterization

Characterization of Nucleotide Binding ­Site-Encoding Genes in Sweetpotato, Ipomoea batatas(L.) Lam., and Their Response to Biotic and Abiotic Stresses

2021 ◽  
pp. 1-15
Author(s):  
Zengzhi Si ◽  
Yake Qiao ◽  
Kai Zhang ◽  
Zhixin Ji ◽  
Jinling Han
Keyword(s):  
Binding Site ◽  
Abiotic Stresses ◽  
Ipomoea Batatas ◽  
Expression Profiles ◽  
Nucleotide Binding ◽  
Regulatory Elements ◽  
Nucleotide Binding Site ◽  
Biotic And Abiotic Stresses ◽  
Encoding Genes

Sweetpotato, <i>Ipomoea batatas</i> (L.) Lam., is an important and widely grown crop, yet its production is affected severely by biotic and abiotic stresses. The nucleotide binding site (NBS)-encoding genes have been shown to improve stress tolerance in several plant species. However, the characterization of NBS-encoding genes in sweetpotato is not well-documented to date. In this study, a comprehensive analysis of NBS-encoding genes has been conducted on this species by using bioinformatics and molecular biology methods. A total of 315 NBS-encoding genes were identified, and 260 of them contained all essential conserved domains while 55 genes were truncated. Based on domain architectures, the 260 NBS-encoding genes were grouped into 6 distinct categories. Phylogenetic analysis grouped these genes into 3 classes: TIR, CC (I), and CC (II). Chromosome location analysis revealed that the distribution of NBS-encoding genes in chromosomes was uneven, with a number ranging from 1 to 34. Multiple stress-related regulatory elements were detected in the promoters, and the NBS-encoding genes’ expression profiles under biotic and abiotic stresses were obtained. According to the bioinformatics analysis, 9 genes were selected for RT-qPCR analysis. The results revealed that <i>IbNBS75</i>, <i>IbNBS219</i>, and <i>IbNBS256</i> respond to stem nematode infection; <i>Ib­NBS240</i>, <i>IbNBS90</i>, and <i>IbNBS80</i> respond to cold stress, while <i>IbNBS208</i>, <i>IbNBS71</i>, and <i>IbNBS159</i> respond to 30% PEG treatment. We hope these results will provide new insights into the evolution of NBS-encoding genes in the sweetpotato genome and contribute to the molecular breeding of sweetpotato in the future.

scholarly journals Molecular characterization of clinical carbapenem-resistant Enterobacterales from Qatar

2021 ◽  
Author(s):  
Fatma Ben Abid ◽  
Clement K. M. Tsui ◽  
Yohei Doi ◽  
Anand Deshmukh ◽  
Christi L. McElheny ◽  
...  
Keyword(s):  
Common Species ◽  
Clinical Samples ◽  
Whole Genome ◽  
E Coli ◽  
Carbapenem Resistant ◽  
Genes Encoding ◽  
Encoding Genes ◽  
Sequence Types ◽  
Common Sequence

AbstractOne hundred forty-nine carbapenem-resistant Enterobacterales from clinical samples obtained between April 2014 and November 2017 were subjected to whole genome sequencing and multi-locus sequence typing. Klebsiella pneumoniae (81, 54.4%) and Escherichia coli (38, 25.5%) were the most common species. Genes encoding metallo-β-lactamases were detected in 68 (45.8%) isolates, and OXA-48-like enzymes in 60 (40.3%). blaNDM-1 (45; 30.2%) and blaOXA-48 (29; 19.5%) were the most frequent. KPC-encoding genes were identified in 5 (3.6%) isolates. Most common sequence types were E. coli ST410 (8; 21.1%) and ST38 (7; 18.4%), and K. pneumoniae ST147 (13; 16%) and ST231 (7; 8.6%).

Sign in / Sign up

Export Citation Format

Share Document