Assessment of genetic stability of the germplasm lines of medicinal plant Scutellaria baicalensis Georgi (Huang-qin) in long-term, in vitro maintained cultures

2007 ◽  
Vol 26 (8) ◽  
pp. 1345-1355 ◽  
Author(s):  
Ali R. Alan ◽  
Hongyan Zeng ◽  
Akym Assani ◽  
Wendy L. Shi ◽  
Hannah E. McRae ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
Genetic Stability ◽  
Scutellaria Baicalensis ◽  
Scutellaria Baicalensis Georgi

scholarly journals In vitro antibacterial activities of Scutellaria baicalensis Georgi against cariogenic bacterial

2007 ◽  
Vol 17 (1) ◽  
pp. 58-64 ◽  
Author(s):  
Chunyan Duan ◽  
Seishi Matsumura ◽  
Naoyuki Kariya ◽  
Michiko Nishimura ◽  
Tsutomu Shimono
Keyword(s):  
Antibacterial Activities ◽  
Scutellaria Baicalensis ◽  
Scutellaria Baicalensis Georgi

Genetic stability and phytochemical profiling of the in vitro regenerated plants of Angelica glauca Edgew.: an endangered medicinal plant of Himalaya

2018 ◽  
Vol 135 (1) ◽  
pp. 111-118 ◽  
Author(s):  
Janhvi Mishra Rawat ◽  
Aakriti Bhandari ◽  
Susmita Mishra ◽  
Balwant Rawat ◽  
Ashok Kumar Dhakad ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
Genetic Stability ◽  
Regenerated Plants ◽  
Endangered Medicinal Plant ◽  
Angelica Glauca

A comparison of genetic stability in tea [Camellia sinensis (L.) Kuntze] plantlets derived from callus with plantlets from long-term in vitro propagation

2019 ◽  
Vol 138 (3) ◽  
pp. 467-474 ◽  
Author(s):  
Lidiia Samarina ◽  
Maya Gvasaliya ◽  
Natalia Koninskaya ◽  
Ruslan Rakhmangulov ◽  
Alexander Efremov ◽  
...  
Keyword(s):  
Camellia Sinensis ◽  
Genetic Stability ◽  
In Vitro Propagation

Genetic Stability in Long-Term Micropropagated Plants ofGentiana Kurroo—An Endangered Medicinal Plant

2009 ◽  
Vol 10 (4) ◽  
pp. 236-244 ◽  
Author(s):  
R. Kaur ◽  
Neelam Panwar ◽  
Bhawna Saxena ◽  
R. Raina ◽  
S. V. Bharadwaj
Keyword(s):  
Medicinal Plant ◽  
Genetic Stability ◽  
Endangered Medicinal Plant ◽  
Micropropagated Plants

The Stimulatory Activities of Baicalein and Baicalin Compounds Derived from Scutellaria baicalensis on Insulin Secretion In Vitro

2012 ◽  
Vol 554-556 ◽  
pp. 1673-1677 ◽  
Author(s):  
Yu Ping Li ◽  
Hu Su ◽  
Xiao Fang Pi ◽  
Yan Chun Gong ◽  
Xiang Yuan Xiong ◽  
...  
Keyword(s):  
Insulin Secretion ◽  
Total Cholesterol Level ◽  
Scutellaria Baicalensis ◽  
Dependent Manner ◽  
Scutellaria Baicalensis Georgi ◽  
Stimulatory Activity ◽  
Atp Levels ◽  
Glucose Stimulated Insulin Secretion ◽  
Insulin Secretion In Vitro

The medicinal plant Scutellaria baicalensis Georgi has been used widely in traditional Chinese medicine for anti-inflammation, anticancer, antiviral and antibacterial infections, reducing the total cholesterol level and decreasing blood pressures. Baicalein and baicalin are two major flavonoid of Scutellaria baicalensis Georgi, exhibit various bioactivities. In the present study, the stimulatory activity of baicalin and baicalein derived from Scutellaria baicalensis on insulin secretion in vitro was investigated using HIT-T15 cell, a Syrian hamster transformed β-cell line. The survival rate of cells treated with baicalin or baicalein (0.01-0.5 mg/ml) increased significantly (P < 0.05). In the presence of 5.6 mM glucose, baicalin or baicalein (0.01-0.5 mg/ml) increased glucose-stimulated insulin secretion and cellular ATP levels in HIT-T15 cells. Baicalin and baicalein exhibited significant stimulatory activity in a dose-dependent manner without apparent cytotoxicity at concentrations less than 0.1 mg/ml. The results obtained in this study suggest that baicalin or baicalein increases the insulin secretion of HIT-T15 cells by the enhancement of β-cells activity and cellular ATP levels. Baicalin or baicalein could be candidates for a new class of anti-diabetic drugs.

UPLC-Q-TOF/MS for Analysis of the Metabolites of Flavone Glycosides from Scutellaria baicalensis Georgi by Human Fecal Flora in Vitro

2013 ◽  
Vol 76 (15-16) ◽  
pp. 975-983 ◽  
Author(s):  
Jun Xu ◽  
Dawei Qian ◽  
Shu Jiang ◽  
Jianming Guo ◽  
Er-xin Shang ◽  
...  
Keyword(s):  
Scutellaria Baicalensis ◽  
Fecal Flora ◽  
Scutellaria Baicalensis Georgi ◽  
Flavone Glycosides ◽  
Tof Ms

scholarly journals MICROPROPAGATION AND PLOIDY STABILITY OF Lippia lacunosa Mart. & Schauer: AN ENDANGERED BRAZILIAN MEDICINAL PLANT

2019 ◽  
Vol 6 (1) ◽  
pp. 1-7
Author(s):  
Diego Pandeló José ◽  
José Marcello Salabert De Campos ◽  
Lyderson Facio Viccini ◽  
Emilly Ruas Alkimim ◽  
Marcelo De Oliveira Santos
Keyword(s):  
Flow Cytometry ◽  
Medicinal Plant ◽  
Dna Content ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Naphthalene Acetic Acid ◽  
Flow Cytometry Analysis ◽  
Ploidy Levels

Lippia lacunosa is a Brazilian savanna plant that belongs to the Verbenaceae family. It has been used in folk medicine as a treatment for different diseases. This species represents an endangered Brazilian medicinal plant, and this is the first report documenting a reliable protocol for the in vitro propagation and regeneration of L. lacunosa. Axenic explants were cultivated in MS medium containing different concentrations of naphthalene acetic acid (NAA) to induce root growth. The mean shoot length and the number of roots were highest with 0.06 mg·L-1 NAA. The highest number of buds in shoot regeneration was induced with 2 mg·L-1 6-benzylaminopurine (BA). To obtain a long-term culture, the dwarf shoots were elongated on MS media containing 0.5 mg·L-1 BA alternated with MS containing 2 mg·L-1 BA every 40 days. In the present protocol, the long-term shoots retained the ability to root even after long periods of BA treatment. In addition, we evaluated the nuclear DNA content and ploidy levels, including the occurrence of endopolyploidy, in long-term micropropagated plant leaves using flow cytometry analysis. The plants propagated in vitro over several years possessed nuclear DNA contents ranging from 2.940 to 3.095 pg, and no differences in DNA content were found among in vitro plants or between these plants and the control (L. lacunosa from a greenhouse with a DNA content of 3.08 pg). The flow cytometry analysis also demonstrated that there was no polyploidization. The present study will be useful for biotechnological approaches and provides the first estimate of the nuclear DNA content of this species using flow cytometry.

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