Naturally occurring high oleic acid cottonseed oil: identification and functional analysis of a mutant allele of Gossypium barbadense fatty acid desaturase-2

Planta ◽  
2016 ◽  
Vol 245 (3) ◽  
pp. 611-622 ◽  
Author(s):  
Jay Shockey ◽  
Michael Dowd ◽  
Brian Mack ◽  
Matthew Gilbert ◽  
Brian Scheffler ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Oleic Acid ◽  
Fatty Acid ◽  
Mutant Allele ◽  
Fatty Acid Desaturase ◽  
Cottonseed Oil ◽  
High Oleic Acid ◽  
High Oleic ◽  
Naturally Occurring ◽  
Fatty Acid Desaturase 2

scholarly journals Two high-oleic-acid soybean mutants, M23 and KK21, have disrupted microsomal omega-6 fatty acid desaturase, encoded by GmFAD2-1a

2008 ◽  
Vol 58 (4) ◽  
pp. 447-452 ◽  
Author(s):  
Toyoaki Anai ◽  
Tomoko Yamada ◽  
Rumiko Hideshima ◽  
Takehito Kinoshita ◽  
Shaikh M. Rahman ◽  
...  
Keyword(s):  
Oleic Acid ◽  
Fatty Acid ◽  
Fatty Acid Desaturase ◽  
High Oleic Acid ◽  
High Oleic ◽  
Omega 6

scholarly journals Transcriptomic Analysis Reveals the High-Oleic Acid Feedback Regulating the Homologous Gene Expression of Stearoyl-ACP Desaturase 2 (SAD2) in Peanuts

2019 ◽  
Vol 20 (12) ◽  
pp. 3091 ◽  
Author(s):  
Hao Liu ◽  
Jianzhong Gu ◽  
Qing Lu ◽  
Haifen Li ◽  
Yanbin Hong ◽  
...  
Keyword(s):  
Gene Expression ◽  
Oleic Acid ◽  
Fatty Acid ◽  
Seed Development ◽  
Fatty Acid Desaturase ◽  
Transcriptomic Analysis ◽  
Homologous Gene ◽  
Oleic Acid Content ◽  
High Oleic Acid ◽  
High Oleic

Peanuts with high oleic acid content are usually considered to be beneficial for human health and edible oil storage. In breeding practice, peanut lines with high monounsaturated fatty acids are selected using fatty acid desaturase 2 (FAD2), which is responsible for the conversion of oleic acid (C18:1) to linoleic acid (C18:2). Here, comparative transcriptomics were used to analyze the global gene expression profile of high- and normal-oleic peanut cultivars at six time points during seed development. First, the mutant type of FAD2 was determined in the high-oleic peanut (H176). The result suggested that early translation termination occurred simultaneously in the coding sequence of FAD2-A and FAD2-B, and the cultivar H176 is capable of utilizing a potential germplasm resource for future high-oleic peanut breeding. Furthermore, transcriptomic analysis identified 74 differentially expressed genes (DEGs) involved in lipid metabolism in high-oleic peanut seed, of which five DEGs encoded the fatty acid desaturase. Aradu.XM2MR belonged to the homologous gene of stearoyl-ACP (acyl carrier protein) desaturase 2 (SAD2) that converted the C18:0 into C18:1. Further subcellular localization studies indicated that FAD2 was located at the endoplasmic reticulum (ER), and Aradu.XM2MR was targeted to the plastid in Arabidopsis protoplast cells. To examine the dynamic mechanism of this finding, we focused on the peroxidase (POD)-mediated fatty acid (FA) degradation pathway. The fad2 mutant significantly increased the POD activity and H2O2 concentration at the early stage of seed development, implying that redox signaling likely acted as a messenger to connect the signaling transduction between the high-oleic content and Aradu.XM2MR transcription level. Taken together, transcriptome analysis revealed the feedback mechanism of SAD2 (Aradu.XM2MR) associated with FAD2 mutation during the seed developmental stage, which could provide a potential peanut breeding strategy based on identified candidate genes to improve the content of oleic acid.

The Interaction of the Soybean Seed High Oleic Acid Oil Trait With Other Fatty Acid Modifications

2018 ◽  
Vol 95 (1) ◽  
pp. 39-49 ◽  
Author(s):  
Kristin Bilyeu ◽  
Mária Škrabišová ◽  
Doug Allen ◽  
Istvan Rajcan ◽  
Debra E. Palmquist ◽  
...  
Keyword(s):  
Oleic Acid ◽  
Fatty Acid ◽  
Soybean Seed ◽  
High Oleic Acid ◽  
Acid Oil ◽  
High Oleic ◽  
Fatty Acid Modifications

scholarly journals Agronomic Traits and Fatty Acid Composition of High‑Oleic Acid Cultivar Hosim

2018 ◽  
Vol 6 (1) ◽  
pp. 44-50 ◽  
Author(s):  
Jeong-Dong Lee ◽  
Minsu Kim ◽  
Krishnanand P. Kulkarni ◽  
Jong Tae Song
Keyword(s):  
Fatty Acid Composition ◽  
Oleic Acid ◽  
Fatty Acid ◽  
Acid Composition ◽  
Agronomic Traits ◽  
High Oleic Acid ◽  
High Oleic
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