Molecular characterization and phylogenetic analyses of Tripartiella macrosoma Basson and Van As, 1987 and Tripartiella obtusa Ergens and Lom, 1970 based on 18S rRNA gene sequence data

2021 ◽  
Author(s):  
Weiwei Qin ◽  
Qian Zhou ◽  
Shiyao Wang ◽  
Fahui Tang ◽  
Yuanjun Zhao
Keyword(s):  
Molecular Characterization ◽  
18S Rrna ◽  
Gene Sequence ◽  
Sequence Data ◽  
Phylogenetic Analyses ◽  
18S Rrna Gene ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Gene Sequence Data

Reassessment of the classification of the Order Haplosclerida (Class Demospongiae, Phylum Porifera) using 18S rRNA gene sequence data

2007 ◽  
Vol 43 (1) ◽  
pp. 344-352 ◽  
Author(s):  
N.E. Redmond ◽  
R.W.M. van Soest ◽  
M. Kelly ◽  
J. Raleigh ◽  
S.A.A. Travers ◽  
...  
Keyword(s):  
18S Rrna ◽  
Gene Sequence ◽  
Sequence Data ◽  
18S Rrna Gene ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Gene Sequence Data

Characterization of two novel yeast strains used in mediated biosensors for wastewater

2002 ◽  
Vol 48 (5) ◽  
pp. 418-426 ◽  
Author(s):  
Steve P Trosok ◽  
John H.T Luong ◽  
David F Juck ◽  
Brian T Driscoll
Keyword(s):  
18S Rrna ◽  
Gene Sequence ◽  
Phylogenetic Analyses ◽  
Pulp Mill ◽  
18S Rrna Gene ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Candida Spp ◽  
Yeast Strains ◽  
Physiological Tests

After isolation from a pulp mill wastewater treatment facility, two yeast strains, designated SPT1 and SPT2, were characterized and used in the development of mediated biochemical oxygen demand (BOD) biosensors for wastewater. 18S rRNA gene sequence analysis revealed a one nucleotide difference between the sequence of SPT1 and those of Candida sojae and Candida viswanthii. While SPT2 had the highest overall homology to Pichia norvegensis, at only 73.5%, it is clearly an ascomycete, based on BLAST comparisons and phylogenetic analyses. Neighbor-joining dendrograms indicated that SPT1 clustered with several Candida spp., and that SPT2 clustered with Starmera spp., albeit as a very deep branch. Physiological tests, microscopic observations, and fatty acid analysis confirmed that SPT1 and SPT2 are novel yeast strains. Physiological tests also indicated that both strains had potential for use in mediated biosensors for estimation of BOD in wastewater. The lower detection limits of SPT1- and SPT2-based K3Fe(CN)6-mediated biosensors for a pulp-mill effluent were 2 and 1 mg BOD/L, respectively. Biosensor-response times for effluents from eight different pulp mills were in the range of 5 min. Reliability and sensitivity of the SPT1- and SPT2-based biosensors were good, but varied with the wastewater.Key words: yeast characterization, 18S rRNA gene sequence, pulp-mill wastewater, BOD5, mediated BOD biosensor.

scholarly journals Intra- and Interindividual Variations Mask Interspecies Variation in the Microbiota of Sympatric Peromyscus Populations

2014 ◽  
Vol 81 (1) ◽  
pp. 396-404 ◽  
Author(s):  
Nielson T. Baxter ◽  
Judy J. Wan ◽  
Alyxandria M. Schubert ◽  
Matthew L. Jenior ◽  
Philip Myers ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
18S Rrna ◽  
Gene Sequence ◽  
Sequence Data ◽  
18S Rrna Gene ◽  
Physiological Status ◽  
Rrna Gene ◽  
Multiple Time ◽  
Rrna Gene Sequence ◽  
Content Type

ABSTRACTUsing populations of two sympatricPeromyscusspecies, we characterized the importance of the host species, physiology, environment, diet, and other factors in shaping the structure and dynamics of their gut microbiota. We performed a capture-mark-release experiment in which we obtained 16S rRNA gene sequence data from 49 animals at multiple time points. In addition, we performed 18S rRNA gene sequencing of the same samples to characterize the diet of each individual. Our analysis could not distinguish between the two species ofPeromyscuson the basis of the structures of their microbiotas. However, we did observe a set of bacterial populations that were found in every animal. Most notable were abundant representatives of the generaLactobacillusandHelicobacter. When we combined the 16S and 18S rRNA gene sequence analyses, we were unable to distinguish the communities on the basis of the animal's diet. Furthermore, there were no discernible differences in the structure of the gut communities based on the capture site or their developmental or physiological status. Finally, in contrast to humans, where each individual has a unique microbiota when sampled over years, among the animals captured in this study, the uniqueness of each microbiota was lost within a week of the original sampling. Wild populations provide an opportunity to study host-microbiota interactions as they originally evolved, and the ability to perform natural experiments will facilitate a greater understanding of the factors that shape the structure and function of the gut microbiota.

scholarly journals Correction to: A comprehensive fungi-specific 18S rRNA gene sequence primer toolkit suited for diverse research issues and sequencing platforms

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Stefanos Banos ◽  
Guillaume Lentendu ◽  
Anna Kopf ◽  
Tesfaye Wubet ◽  
Frank Oliver Glöckner ◽  
...  
Keyword(s):  
Fungal Community ◽  
18S Rrna ◽  
Gene Sequence ◽  
18S Rrna Gene ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Community Surveys ◽  
Research Issues ◽  
Nomenclature System ◽  
Sequencing Platforms

Following publication of the original article [1], we have been notified that three of the primer names identified as most promising candidates for fungal community surveys were incorrectly renamed following the primer nomenclature system proposed by Gargas & DePriest [2].

scholarly journals Taxonomy of Australian clinical isolates of the genus Photorhabdus and proposal of Photorhabdus asymbiotica subsp. asymbiotica subsp. nov. and P. asymbiotica subsp. australis subsp. nov.

2004 ◽  
Vol 54 (4) ◽  
pp. 1301-1310 ◽  
Author(s):  
R. J. Akhurst ◽  
N. E. Boemare ◽  
P. H. Janssen ◽  
M. M. Peel ◽  
D. A. Alfredson ◽  
...  
Keyword(s):  
16S Rrna ◽  
Dna Hybridization ◽  
Gene Sequence ◽  
Sequence Data ◽  
Phylogenetic Analyses ◽  
Clinical Isolates ◽  
Rrna Gene ◽  
Clinical Specimens ◽  
The Usa ◽  
Gene Sequence Data

The relationship of Photorhabdus isolates that were cultured from human clinical specimens in Australia to Photorhabdus asymbiotica isolates from human clinical specimens in the USA and to species of the genus Photorhabdus that are associated symbiotically with entomopathogenic nematodes was evaluated. A polyphasic approach that involved DNA–DNA hybridization, phylogenetic analyses of 16S rRNA and gyrB gene sequences and phenotypic characterization was adopted. These investigations showed that gyrB gene sequence data correlated well with DNA–DNA hybridization and phenotypic data, but that 16S rRNA gene sequence data were not suitable for defining species within the genus Photorhabdus. Australian clinical isolates proved to be related most closely to clinical isolates from the USA, but the two groups were distinct. A novel subspecies, Photorhabdus asymbiotica subsp. australis subsp. nov. (type strain, 9802892T=CIP 108025T=ACM 5210T), is proposed, with the concomitant creation of Photorhabdus asymbiotica subsp. asymbiotica subsp. nov. Analysis of gyrB sequences, coupled with previously published data on DNA–DNA hybridization and PCR-RFLP analysis of the 16S rRNA gene, indicated that there are more than the three subspecies of Photorhabdus luminescens that have been described and confirmed the validity of the previously proposed subdivision of Photorhabdus temperata. Although a non-luminescent, symbiotic isolate clustered consistently with P. asymbiotica in gyrB phylogenetic analyses, DNA–DNA hybridization indicated that this isolate does not belong to the species P. asymbiotica and that there is a clear distinction between symbiotic and clinical species of Photorhabdus.

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