Enhanced amperometric response of a glucose oxidase and horseradish peroxidase based bienzyme glucose biosensor modified with a film of polymerized toluidine blue containing reduced graphene oxide

2015 ◽  
Vol 182 (11-12) ◽  
pp. 1949-1956 ◽  
Author(s):  
Fang Wang ◽  
Wencheng Gong ◽  
Lili Wang ◽  
Zilin Chen
Keyword(s):  
Graphene Oxide ◽  
Horseradish Peroxidase ◽  
Glucose Oxidase ◽  
Reduced Graphene Oxide ◽  
Toluidine Blue ◽  
Glucose Biosensor ◽  
Amperometric Response ◽  
Reduced Graphene

scholarly journals Electrodeposition–Assisted Assembled Multilayer Films of Gold Nanoparticles and Glucose Oxidase onto Polypyrrole-Reduced Graphene Oxide Matrix and Their Electrocatalytic Activity toward Glucose

Nanomaterials ◽  
2018 ◽  
Vol 8 (12) ◽  
pp. 993 ◽  
Author(s):  
Baoyan Wu ◽  
Shihua Hou ◽  
Yongyong Xue ◽  
Zhan Chen
Keyword(s):  
Gold Nanoparticles ◽  
Graphene Oxide ◽  
Glucose Oxidase ◽  
Reduced Graphene Oxide ◽  
Self Assembly ◽  
Electrocatalytic Activity ◽  
Multilayer Films ◽  
Glucose Biosensor ◽  
Future Design ◽  
Reduced Graphene

The study reports a facile and eco-friendly approach for nanomaterial synthesis and enzyme immobilization. A corresponding glucose biosensor was fabricated by immobilizing the gold nanoparticles (AuNPs) and glucose oxidase (GOD) multilayer films onto the polypyrrole (PPy)/reduced graphene oxide (RGO) modified glassy carbon electrode (GCE) via the electrodeposition and self-assembly. PPy and graphene oxide were first coated on the surface of a bare GCE by the electrodeposition. Then, AuNPs and GOD were alternately immobilized onto PPy-RGO/GCE electrode using the electrodeposition of AuNPs and self-assembly of GOD to obtain AuNPs-GOD multilayer films. The resulting PPy-RGO-(AuNPs-GOD)n/GCE biosensors were used to characterize and assess their electrocatalytic activity toward glucose using cyclic voltammetry and amperometry. The response current increased with the increased number of AuNPs-GOD layers, and the biosensor based on four layers of AuNPs-GOD showed the best performance. The PPy-RGO-(AuNPs-GOD)4/GCE electrode can detect glucose in a linear range from 0.2 mM to 8 mM with a good sensitivity of 0.89 μA/mM, and a detection limit of 5.6 μM (S/N = 3). This study presents a promising eco-friendly biosensor platform with advantages of electrodeposition and self-assembly, and would be helpful for the future design of more complex electrochemical detection systems.

Fabrication of a novel disposable glucose biosensor using an electrochemically reduced graphene oxide–glucose oxidase biocomposite

2016 ◽  
Vol 8 (38) ◽  
pp. 6974-6981 ◽  
Author(s):  
Kathiresan Vijayaraj ◽  
Suck Won Hong ◽  
Sung-Ho Jin ◽  
Seung-Cheol Chang ◽  
Deog-Su Park
Keyword(s):  
Graphene Oxide ◽  
Glucose Oxidase ◽  
Reduced Graphene Oxide ◽  
Electrochemical Method ◽  
Graphite Electrode ◽  
Cost Effective ◽  
Glucose Biosensor ◽  
Pencil Graphite Electrode ◽  
Reduced Graphene ◽  
Electrochemically Reduced Graphene Oxide

A disposable glucose biosensor has been fabricated on the surface of a cost-effective pencil graphite electrode (PGE) by an electrochemical method, using glucose oxidase (GOx) and reduced graphene oxide (rGO).

Direct electrochemistry of glucose oxidase immobilized on ZrO2 nanoparticles-decorated reduced graphene oxide sheets for a glucose biosensor

RSC Advances ◽  
2014 ◽  
Vol 4 (57) ◽  
pp. 30358-30367 ◽  
Author(s):  
A. T. Ezhil Vilian ◽  
Shen-Ming Chen ◽  
M. Ajmal Ali ◽  
Fahad M. A. Al-Hemaid
Keyword(s):  
Graphene Oxide ◽  
Glucose Oxidase ◽  
Reduced Graphene Oxide ◽  
Direct Electrochemistry ◽  
Glucose Biosensor ◽  
Preparation Procedure ◽  
Zro2 Nanoparticles ◽  
Schematic Representation ◽  
Reduced Graphene ◽  
Graphene Oxide Sheets

Schematic representation of the preparation procedure of GOx–PLL/RGO–ZrO2 composite.

Amperometric Glucose Biosensor Based on AuPd Modified Reduced Graphene Oxide/Polyimide Film with Glucose Oxidase

2017 ◽  
Vol 164 (6) ◽  
pp. B285-B291 ◽  
Author(s):  
Xuan Shen ◽  
Xiaohong Xia ◽  
Yongling Du ◽  
Weichun Ye ◽  
Chunming Wang
Keyword(s):  
Graphene Oxide ◽  
Glucose Oxidase ◽  
Reduced Graphene Oxide ◽  
Polyimide Film ◽  
Glucose Biosensor ◽  
Reduced Graphene

Reduced graphene oxide-nickel nanoparticles/biopolymer composite films for the sub-millimolar detection of glucose

The Analyst ◽  
2016 ◽  
Vol 141 (13) ◽  
pp. 4151-4161 ◽  
Author(s):  
Rahul Krishna ◽  
José M. Campiña ◽  
Paula M. V. Fernandes ◽  
João Ventura ◽  
Elby Titus ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Glucose Oxidase ◽  
Reduced Graphene Oxide ◽  
Nickel Nanoparticles ◽  
Composite Films ◽  
Glucose Biosensor ◽  
Reduced Graphene ◽  
First Time

A conjugate of reduced graphene oxide and nickel nanoparticles was synthesized and deposited onto GCE as a nanocomposite of chitosan and glucose oxidase. Applications as a glucose biosensor were explored for the first time.

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