scholarly journals Genetic diversity of the movement and coat protein genes of South American isolates of Prunus necrotic ringspot virus

2008 ◽  
Vol 153 (5) ◽  
pp. 909-919 ◽  
Author(s):  
Nicola Fiore ◽  
Thor V. M. Fajardo ◽  
Simona Prodan ◽  
María Carmen Herranz ◽  
Frederic Aparicio ◽  
...  
2012 ◽  
Vol 160 (10) ◽  
pp. 603-605 ◽  
Author(s):  
Elżbieta Kalinowska ◽  
Elżbieta Paduch-Cichal ◽  
Maria Chodorska

2013 ◽  
Vol 24 (2) ◽  
pp. 235-241 ◽  
Author(s):  
Saurabh Kulshrestha ◽  
Vipin Hallan ◽  
Anshul Sharma ◽  
Chandrika Attri Seth ◽  
Anjali Chauhan ◽  
...  

PLoS ONE ◽  
2017 ◽  
Vol 12 (6) ◽  
pp. e0179284 ◽  
Author(s):  
Wycliff M. Kinoti ◽  
Fiona E. Constable ◽  
Narelle Nancarrow ◽  
Kim M. Plummer ◽  
Brendan Rodoni

2006 ◽  
Vol 87 (6) ◽  
pp. 1745-1750 ◽  
Author(s):  
Frederic Aparicio ◽  
Jesús A. Sánchez-Navarro ◽  
Vicente Pallás

Interactions between viral proteins are critical for virus viability. Bimolecular fluorescent complementation (BiFC) technique determines protein interactions in real-time under almost normal physiological conditions. The coat protein (CP) of Prunus necrotic ringspot virus is required for multiple functions in its replication cycle. In this study, the region involved in CP dimerization has been mapped by BiFC in both bacteria and plant tissue. Full-length and C-terminal deleted forms of the CP gene were fused in-frame to the N- and C-terminal fragments of the yellow fluorescent protein. The BiFC analysis showed that a domain located between residues 9 and 27 from the C-end plays a critical role in dimerization. The importance of this C-terminal region in dimer formation and the applicability of the BiFC technique to analyse viral protein interactions are discussed.


Virology ◽  
2003 ◽  
Vol 313 (1) ◽  
pp. 213-223 ◽  
Author(s):  
Frederic Aparicio ◽  
Marçal Vilar ◽  
Enrique Perez-Payá ◽  
Vicente Pallás

2013 ◽  
Vol 24 (1) ◽  
pp. 70-73 ◽  
Author(s):  
M. S. Akhter ◽  
Y. B. Basavaraj ◽  
A. M. Akanda ◽  
B. Mandal ◽  
R. K. Jain

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