Molecular characterization of two potato virus S isolates from late-blight-resistant genotypes of potato (Solanum tuberosum)

2009 ◽  
Vol 154 (11) ◽  
pp. 1861-1863 ◽  
Author(s):  
Y. H. Lin ◽  
K. L. Druffel ◽  
J. Whitworth ◽  
M. J. Pavek ◽  
Hanu R. Pappu
Keyword(s):  
Solanum Tuberosum ◽  
Late Blight ◽  
Molecular Characterization ◽  
Potato Virus ◽  
Potato Virus S ◽  
Resistant Genotypes

Biological and Molecular Characterization of Potato virus Y Infecting Potato (Solanum tuberosum) in India

2013 ◽  
Vol 6 (5) ◽  
pp. 257-264 ◽  
Author(s):  
Pooja Sharma ◽  
Anurag Kumar Sahu ◽  
Rakesh Kumar Verma ◽  
Ritesh Mishra ◽  
R.K. Gaur
Keyword(s):  
Solanum Tuberosum ◽  
Molecular Characterization ◽  
Potato Virus ◽  
Potato Virus Y

Molecular characterization of domestic and exotic potato virus S isolates and a global analysis of genomic sequences

2014 ◽  
Vol 159 (8) ◽  
pp. 2115-2122 ◽  
Author(s):  
Y.-H. Lin ◽  
J. A. Abad ◽  
C. J. Maroon-Lango ◽  
K. L. Perry ◽  
H. R. Pappu
Keyword(s):  
Molecular Characterization ◽  
Potato Virus ◽  
Global Analysis ◽  
Genomic Sequences ◽  
Potato Virus S

Isolation and molecular characterization of a new vegetative MADS-box gene from Solanum tuberosum L.

Planta ◽  
1998 ◽  
Vol 207 (2) ◽  
pp. 181-188 ◽  
Author(s):  
María José Carmona ◽  
Nely Ortega ◽  
Federico Garcia-Maroto
Keyword(s):  
Solanum Tuberosum ◽  
Molecular Characterization ◽  
Solanum Tuberosum L ◽  
Mads Box ◽  
Mads Box Gene

scholarly journals Detection and molecular characterization of Slovak tomato isolates belonging to two recombinant strains of potato virus Y

2016 ◽  
Vol 60 (04) ◽  
pp. 347-353 ◽  
Author(s):  
N. SIHELSKÁ ◽  
L. PREDAJŇA ◽  
A. NAGYOVÁ ◽  
K. ŠOLTYS ◽  
J. BUDIŠ ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Potato Virus ◽  
Potato Virus Y ◽  
Recombinant Strains

scholarly journals Detection and genome characterization of Potato virus Y isolates infecting potato (Solanum tuberosum L.) in La Union (Antioquia, Colombia)

2016 ◽  
Vol 34 (3) ◽  
pp. 317-328 ◽  
Author(s):  
Pablo Gutiérrez S. ◽  
Mauricio Marín M. ◽  
Daniel Muñoz E.
Keyword(s):  
Solanum Tuberosum ◽  
Potato Virus ◽  
Potato Virus Y ◽  
Seed Tuber ◽  
Rt Pcr ◽  
Potato Plants ◽  
Nextgeneration Sequencing ◽  
Prevalent Strain ◽  
Detailed Molecular Analysis

Potato virus Y (PVY) is one of the most severe viruses affecting the production of potato (Solanum tuberosum) in the world. This study presents a detailed molecular analysis using nextgeneration sequencing (NGS), IC-RT-qPCR and RT-PCR on the PVY isolates infecting seed-tubers and foliage of potato plants cv. Diacol-Capiro in La Union (Antioquia, Colombia). Analysis of incidence by IC-RT-qPCR in 15 random leaf samples of three cultivation plots and fifteen sprouting tuber eye-buds reveal infection levels between 13.4 and 80%; a higher incidence of 86.7% was observed in seed-tuber samples with threshold cycle (Ct) values as low as 24.3. Genome assembly from a bulk of foliage samples resulted in a consensus PVY genome (PVY_LaUnionF) of 9,702 nt and 399 polymorphic sites within the polyprotein ORF; while the assembled genome from sprouts of tubers has 9,704 nt (PVY_LaUnionT) and contained only six polymorphic nucleotide sites. Phylogenetic analysis demonstrates that the PVY isolates from leaf samples are in the recombinant PVYNTN group (sequence identity >99%); while those from tuber sprouts are in the PVYN/NTN group with identities above 95%. Sanger sequencing of viral capsid suggests the presence of a third variant related to PVYO, a prevalent strain reported in potato fields worldwide.

scholarly journals Characterization of Phytophthora infestans in Wisconsin from 1993 to 1995

Plant Disease ◽  
1998 ◽  
Vol 82 (4) ◽  
pp. 434-436 ◽  
Author(s):  
K. D. Marshall-Farrar ◽  
M. McGrath ◽  
R. V. James ◽  
W. R. Stevenson
Keyword(s):  
Solanum Tuberosum ◽  
Late Blight ◽  
Phytophthora Infestans ◽  
Mating Type ◽  
Production Problem ◽  
Significant Production ◽  
Allozyme Genotype ◽  
Late Blight Of Potato

Late blight of potato (Solanum tuberosum), caused by Phytophthora infestans, recently reappeared in Wisconsin and was a significant production problem in 1994. P. infestans isolates collected in Wisconsin from 1993 to 1995 were characterized for the following traits: mating type, sensitivity to metalaxyl, and allozyme genotype for Glucose-6-phosphate isomerase (Gpi). Characterization of these isolates revealed that a new, more aggressive population (A2 mating type, metalaxyl resistant, and Gpi genotype 100/111/122) is displacing the old population (A1 mating type, metalaxyl sensitive, and Gpi genotype 86/100) in Wisconsin.

Effect of Potato Virus S Infection on Late Blight Resistance in Potato

2014 ◽  
Vol 91 (6) ◽  
pp. 642-648 ◽  
Author(s):  
Yu-Hsuan Lin ◽  
Dennis A. Johnson ◽  
Hanu R. Pappu
Keyword(s):  
Late Blight ◽  
Potato Virus ◽  
Late Blight Resistance ◽  
Blight Resistance ◽  
Potato Virus S

Molecular characterization of potato virus Y resistance in potato (Solanum tuberosumL.)

2015 ◽  
Vol 75 (3) ◽  
pp. 389 ◽  
Author(s):  
Vinay Bhardwaj ◽  
Reena Sharma ◽  
Dalamu ◽  
A. K. Srivastava ◽  
R. Baswaraj ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Potato Virus ◽  
Potato Virus Y

Molecular Characterization of Potato leafroll virus, Potato virus A, and Potato virus X Isolates from Potatoes in Alaskan Cities and Villages

2011 ◽  
Vol 12 (1) ◽  
pp. 39 ◽  
Author(s):  
Nancy L. Robertson ◽  
Jeffrey Smeenk ◽  
Jodie M. Anderson
Keyword(s):  
Molecular Characterization ◽  
Potato Virus ◽  
Potato Virus X ◽  
Potato Leafroll Virus ◽  
First Report ◽  
Potato Virus A ◽  
Potato Viruses ◽  
The World ◽  
Leafroll Virus

Although all three viruses are commonly found in potatoes throughout the world, this is the first report of potato viruses from Alaska to be sequenced and molecularly analyzed for comparisons with known viruses. Accepted for publication 17 January 2011. Published 9 February 2011.

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