scholarly journals Development and evaluation of a one-step real-time RT-PCR assay for universal detection of influenza A viruses from avian and mammal species

2010 ◽  
Vol 155 (5) ◽  
pp. 665-673 ◽  
Author(s):  
Alexander Nagy ◽  
Veronika Vostinakova ◽  
Zuzana Pirchanova ◽  
Lenka Cernikova ◽  
Zuzana Dirbakova ◽  
...  
Keyword(s):  
Real Time ◽  
Influenza A ◽  
Rt Pcr ◽  
Influenza A Viruses ◽  
Pcr Assay ◽  
Mammal Species ◽  
One Step ◽  
Universal Detection

scholarly journals Development and application of a real-time RT-PCR assay to rapidly detect H2 subtype avian influenza A viruses

2021 ◽  
pp. 104063872199481
Author(s):  
Yixin Xiao ◽  
Fan Yang ◽  
Fumin Liu ◽  
Hangping Yao ◽  
Nanping Wu ◽  
...  
Keyword(s):  
Avian Influenza ◽  
Real Time ◽  
Influenza A ◽  
Minor Groove Binder ◽  
Rt Pcr ◽  
Influenza A Viruses ◽  
Pcr Assay ◽  
Infectious Dose ◽  
The Matrix ◽  
Taqman Minor Groove Binder

The H2 subtypes of avian influenza A viruses (avian IAVs) have been circulating in poultry, and they have the potential to infect humans. Therefore, establishing a method to quickly detect this subtype is pivotal. We developed a TaqMan minor groove binder real-time RT-PCR assay that involved probes and primers based on conserved sequences of the matrix and hemagglutinin genes. The detection limit of this assay was as low as one 50% egg infectious dose (EID50)/mL per reaction. This assay is specific, sensitive, and rapid for detecting avian IAV H2 subtypes.

scholarly journals Development of a multiplex real-time RT-PCR that allows universal detection of influenza A viruses and simultaneous typing of influenza A/H1N1/2009 virus

2010 ◽  
Vol 163 (2) ◽  
pp. 258-261 ◽  
Author(s):  
Rory Gunson ◽  
Alasdair Maclean ◽  
Eleri Davies ◽  
Susan Bennett ◽  
Rhona Miller ◽  
...  
Keyword(s):  
Real Time ◽  
Influenza A ◽  
Rt Pcr ◽  
Influenza A Viruses ◽  
Influenza A H1n1 ◽  
Universal Detection

scholarly journals Detection of a novel avian influenza A (H7N9) virus in humans by multiplex one-step real-time RT-PCR assay

2014 ◽  
Vol 14 (1) ◽  
Author(s):  
Jian Fan ◽  
David Cui ◽  
Siuying Lau ◽  
Guoliang Xie ◽  
Xichao Guo ◽  
...  
Keyword(s):  
Avian Influenza ◽  
Real Time ◽  
Influenza A ◽  
H7n9 Virus ◽  
Rt Pcr ◽  
Pcr Assay ◽  
One Step

A one-step multiplex real-time RT-PCR assay for rapid and simultaneous detection of human norovirus genogroup I, II and IV

2013 ◽  
Vol 189 (2) ◽  
pp. 277-282 ◽  
Author(s):  
Yong Yan ◽  
Heng-hui Wang ◽  
Lei Gao ◽  
Ji-mei Ji ◽  
Zhi-jie Ge ◽  
...  
Keyword(s):  
Real Time ◽  
Simultaneous Detection ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Human Norovirus ◽  
One Step

scholarly journals Development of a Diagnostic System for Novel Influenza A(H7N9) Virus Using a Real-Time RT-PCR Assay in Japan

2015 ◽  
Vol 68 (2) ◽  
pp. 113-118 ◽  
Author(s):  
Ikuyo Takayama ◽  
Hitoshi Takahashi ◽  
Mina Nakauchi ◽  
Shiho Nagata ◽  
Masato Tashiro ◽  
...  
Keyword(s):  
Real Time ◽  
Influenza A ◽  
Diagnostic System ◽  
H7n9 Virus ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Novel Influenza A

Rapid detection of Enterovirus and Coxsackievirus A10 by a TaqMan based duplex one-step real time RT-PCR assay

2017 ◽  
Vol 33 ◽  
pp. 8-10 ◽  
Author(s):  
Jingfang Chen ◽  
Rusheng Zhang ◽  
Xinhua Ou ◽  
Dong Yao ◽  
Zheng Huang ◽  
...  
Keyword(s):  
Real Time ◽  
Rapid Detection ◽  
Rt Pcr ◽  
Pcr Assay ◽  
One Step
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