Frequent detection and characterization of hepatitis E virus variants in wild rats (Rattus rattus) in Indonesia

2012 ◽  
Vol 158 (1) ◽  
pp. 87-96 ◽  
Author(s):  
Mulyanto ◽  
Sulaiman Ngongu Depamede ◽  
Made Sriasih ◽  
Masaharu Takahashi ◽  
Shigeo Nagashima ◽  
...  
2014 ◽  
Vol 185 ◽  
pp. 92-102 ◽  
Author(s):  
Suljid Jirintai ◽  
Tanggis ◽  
Mulyanto ◽  
Joseph Benedictus Suparyatmo ◽  
Masaharu Takahashi ◽  
...  

Viruses ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 1385
Author(s):  
Giulia Pezzoni ◽  
Lidia Stercoli ◽  
Eleonora Pegoiani ◽  
Emiliana Brocchi

To evaluate the antigenic properties of Hepatitis E Virus (HEV) Open Reading Frame 2 and 3 (ORF2 and ORF3) codified proteins, we expressed different portions of ORF2 and the entire ORF3 in E. coli, a truncated ORF2, was also expressed in baculovirus. A panel of 37 monoclonal antibodies (MAbs) was raised against ORF2 (1–660 amino acids) and MAbs were mapped and characterized using the ORF2 expressed portions. Selected HEV positive and negative swine sera were used to evaluate ORF2 and ORF3 antigens’ immunogenicity. The MAbs were clustered in six groups identifying six antigenic regions along the ORF2. Only MAbs binding to the sixth ORF2 antigenic region (394–608 aa) were found to compete with HEV positive sera and efficiently catch the recombinant antigen expressed in baculovirus. The ORF2 portion from 394–608 aa demonstrated to include most immunogenic epitopes with 85% of HEV positive swine sera reacting against the region from 461–544 aa. Only 5% of the selected HEV sera reacted against the ORF3 antigen.


2012 ◽  
Vol 43 (3) ◽  
pp. 640-644 ◽  
Author(s):  
Anuwat Wiratsudakul ◽  
Ladawan Sariya ◽  
Phirom Prompiram ◽  
Siriporn Tantawet ◽  
Duangkhamol Suraruangchai ◽  
...  

2017 ◽  
Vol 9 (2) ◽  
pp. 208-218 ◽  
Author(s):  
Oksana Mykytczuk ◽  
Jennifer Harlow ◽  
Sabah Bidawid ◽  
Nathalie Corneau ◽  
Neda Nasheri

Virus Genes ◽  
2016 ◽  
Vol 52 (5) ◽  
pp. 738-742 ◽  
Author(s):  
Hyun-Woo Moon ◽  
Byung-Woo Lee ◽  
Haan Woo Sung ◽  
Byung-Il Yoon ◽  
Hyuk Moo Kwon

2021 ◽  
pp. 109275
Author(s):  
Milagros Virhuez Mendoza ◽  
Kenzo Yonemitsu ◽  
Keita Ishijima ◽  
Shohei Minami ◽  
Supriyono ◽  
...  

Author(s):  
Mohamed Boumaiza ◽  
Khaled Trabelsi ◽  
Zeineb Choucha ◽  
Ines Akrouti ◽  
Serena Leone ◽  
...  

2007 ◽  
Vol 38 (4) ◽  
pp. 693-698 ◽  
Author(s):  
Helder Henrique Paiva ◽  
Valentina Tzaneva ◽  
Rodrigo Haddad ◽  
Jonny Yokosawa

1996 ◽  
Vol 70 (1) ◽  
pp. 207-216 ◽  
Author(s):  
S Jameel ◽  
M Zafrullah ◽  
M H Ozdener ◽  
S K Panda

2020 ◽  
Vol 12 (2) ◽  
pp. 137-147 ◽  
Author(s):  
Sophia Beyer ◽  
Regine Szewzyk ◽  
Regina Gnirss ◽  
Reimar Johne ◽  
Hans-Christoph Selinka

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