scholarly journals Sequence and phylogenetic analysis of nucleocapsid genes of porcine epidemic diarrhea virus (PEDV) strains in China

2013 ◽  
Vol 158 (6) ◽  
pp. 1267-1273 ◽  
Author(s):  
Zhili Li ◽  
Feng Chen ◽  
Yao Yuan ◽  
Xiduo Zeng ◽  
Zhongyan Wei ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Porcine Epidemic Diarrhea Virus ◽  
Diarrhea Virus ◽  
Porcine Epidemic Diarrhea

scholarly journals Molecular Characterization and Phylogenetic Analysis of New Variants of the Porcine Epidemic Diarrhea Virus in Gansu, China in 2012

Viruses ◽  
2013 ◽  
Vol 5 (8) ◽  
pp. 1991-2004 ◽  
Author(s):  
Yufei Tian ◽  
Zhijun Yu ◽  
Kaihui Cheng ◽  
Yuxiu Liu ◽  
Jing Huang ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Molecular Characterization ◽  
Porcine Epidemic Diarrhea Virus ◽  
Diarrhea Virus ◽  
Porcine Epidemic Diarrhea

scholarly journals Molecular Characterization of a Variant Subgroup GII-a Porcine Epidemic Diarrhea Virus Strain in China

2020 ◽  
Author(s):  
Zhige Tian ◽  
Yuexiao Lian ◽  
Xiaoliang Hu ◽  
Yue Shi ◽  
Feng Cong
Keyword(s):  
Phylogenetic Analysis ◽  
Porcine Epidemic Diarrhea Virus ◽  
Genetic Characterization ◽  
Economic Losses ◽  
Recombination Analysis ◽  
Essential Information ◽  
Diarrhea Virus ◽  
Newborn Piglets ◽  
Porcine Epidemic Diarrhea

Abstract Background: Since 2010, variant porcine epidemic diarrhea virus (PEDV) has emerged in several provinces of China. The variant strain causes high mortality of newborn piglets, which has resulted in severe economic losses to the pork breeding industry.Results: A variant PEDV strain, SC-YB73, was isolated and identified in China. Pathological observation showed atrophy of villi and edema in the lamina propria. Sequence analysis indicated that six nucleotides were inserted in the E gene, which was first detected in PEDV strains. Furthermore, fifty nucleotide sites were unique in SC-YB73, when compared with another 28 PEDV strains. Phylogenetic analysis based on the complete genome showed that SC-YB73 was clustered in the variant subgroup GII-a, which is widely prevalent in the pig population in China. Recombination analysis suggested that SC-YB73 originated from the recombination of GDS47, TW/Yunlin550/2018, and COL/Cundinamarca/2014.Conclusion: The present study involved the isolation and genetic characterization of a variant PEDV strain. It will provide essential information for the control of PED outbreaks in China.

scholarly journals Isolation and evolutionary analyses of porcine epidemic diarrhea virus in Asia

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e10114
Author(s):  
Wan Liang ◽  
Danna Zhou ◽  
Chao Geng ◽  
Keli Yang ◽  
Zhengying Duan ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Porcine Epidemic Diarrhea Virus ◽  
Virus Isolation ◽  
Vaccine Development ◽  
Evolutionary Analysis ◽  
Genetic Characteristics ◽  
S Protein ◽  
Diarrhea Virus ◽  
Comparative Genomics Analysis ◽  
Porcine Epidemic Diarrhea

Porcine epidemic diarrhea virus (PEDV) is a leading cause of diarrhea in pigs worldwide. Virus isolation and genetic evolutionary analysis allow investigations into the prevalence of epidemic strains and provide data for the clinical diagnosis and vaccine development. In this study, we investigated the genetic characteristics of PEDV circulation in Asia through virus isolation and comparative genomics analysis. APEDV strain designated HB2018 was isolated from a pig in a farm experiencing a diarrhea outbreak. The complete genome sequence of HB2018 was 28,138 bp in length. Phylogenetic analysis of HB2018 and 207 PEDVs in Asia showed that most PEDV strains circulating in Asia after 2010 belong to genotype GII, particularly GII-a. The PEDV vaccine strain CV777 belonged to GI, and thus, unmatched genotypes between CV777 and GII-a variants might partially explain incomplete protection by the CV777-derived vaccine against PEDV variants in China. In addition, we found the S protein of variant strains contained numerous mutations compared to the S protein of CV777, and these mutations occurred in the N-terminal domain of the S protein. These mutations may influence the antigenicity, pathogenicity, and neutralization properties of the variant strains.

scholarly journals Genetic characterization and phylogenetic analysis of porcine epidemic diarrhea virus in Guangdong, China, between 2018 and 2019

PLoS ONE ◽  
2021 ◽  
Vol 16 (6) ◽  
pp. e0253622
Author(s):  
Feng Wen ◽  
Jing Yang ◽  
Anqi Li ◽  
Zhonggui Gong ◽  
Lulu Yang ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Porcine Epidemic Diarrhea Virus ◽  
Southern China ◽  
Guangdong Province ◽  
Clinical Samples ◽  
Transmissible Gastroenteritis Virus ◽  
Genetic Characteristics ◽  
Common Amino Acid ◽  
Diarrhea Virus ◽  
Porcine Epidemic Diarrhea

Porcine epidemic diarrhea virus (PEDV), a leading cause of piglet diarrhea outbreaks, poses a significant danger to the swine industry. The aim of this study was to investigate the epidemic characteristics of PEDV that was circulating in Guangdong province, one of China’s major pig producing provinces. Clinical samples were collected from eight pig farms in Guangdong province between 2018 and 2019 and tested for the major porcine enteric pathogens, including PEDV, transmissible gastroenteritis virus (TGEV), Swine enteric coronavirus (SeCoV), Swine acute diarrhea syndrome coronavirus (SADS-CoV), porcine deltacoronavirus (PDCoV), and porcine rotavirus (RV). As a result, only PEDV and RV were detected at a rate of 47.0% (16/34) and 18.6% (8/34), respectively. Coinfectoin with PEDV and RV occurred at a rate of PEDV 12.5% (2/16). Subsequently, the full-length S gene sequences of 13 PEDV strains were obtained, and phylogenetic analysis suggested the presence of GII-c group PEDV strains in this region (non-S-INDEL). Two novel common amino acid insertions (55T/IG56 and 551L) and one novel glycosylation site (1199G+) were detected when the CV777 and ZJ08 vaccine strains were compared. Furthermore, intragroup recombination events in the S gene regions 51–548 and 2478–4208 were observed in the PEDV strains studied. In summary, the observations provide current information on the incidence of viral agents causing swine diarrhea in southern China and detailed the genetic characteristics and evolutionary history of the dominant PEDV field strains. Our findings will aid in the development of an updated vaccine for the prevention and control of PEDV variant strains.

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