The effect of auxin (indole-3-acetic acid) on the growth rate and tropism of the sporangiophore of Phycomyces blakesleeanus and identification of auxin-related genes

Slow growth rate of plantlets, few micro-shoots per explant, and slow root growth rate are restrictions of in vitro propagation of poinsettia (Euphorbia pulcherrima Willd. ex Koltz). The purpose of this research was to develop an efficient in vitro proliferation technique for poinsettia ‘Prestige™ Red’. Explants (apical buds and axillary buds) placed on Murashige and Skoog (MS) basal medium containing only 6-benzylaminopurine (BA) and combinations of BA and indole-3-acetic acid (IAA) mostly produced red callus, which is productive and some white and gray–green calluses at the base of plantlets after 1 month, whereas explants in a medium without plant growth regulators (PGRs) produced no callus. Addition of IAA into the rooting medium increased rooting efficiency; plantlets grown in half-strength MS salts and vitamins with 28.5 μM IAA initiated rooting 11 days earlier than the plantlets grown with no PGRs. Optimization of PGR concentrations during poinsettia micropropagation helped resolve previous restrictions of in vitro poinsettia proliferation. Chemical names used: 6-benzylaminopurine (BA); indole-3-acetic acid (IAA)

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Promotion of in Vitro Leaf Growth of Inner Scales Excised from Dormant Onion Bulbs

HortScience ◽

10.21273/hortsci.25.2.228 ◽

1990 ◽

Vol 25 (2) ◽

pp. 228-229

Author(s):

Kil Sun Yoo ◽

Leonard M. Pike ◽

B. Greg Cobb

Keyword(s):

Growth Rate ◽

Acetic Acid ◽

Abscisic Acid ◽

Gibberellic Acid ◽

Allium Cepa ◽

Leaf Growth ◽

Inhibitory Effects ◽

Short Day ◽

Indole 3 Acetic Acid

Inner scales excised from dormant bulbs of the short-day `Texas Grano 1015Y' onion (Allium cepa L.) were cultured in vitro and leaf growth was examined. Light promoted leaf growth, but no differences in leaf growth were observed for media pH between 4 and 7. Leaf growth rate in darkness was highest at 24C, reduced at 15C, and greatly reduced at SC. Kinetin promoted leaf growth at 1, 10, and 100 μm. IAA was effective at 1 and 10 μM, but not at 0.1 and 100 μm. GA3 promoted growth at 0.1 μM. No inhibitory effects of ABA on leaf growth could be detected. Chemical names used: 1-H-indole-3-acetic acid (IAA), abscisic acid (ABA), gibberellic acid (GA3), 6-furfurylaminopurine (Kinetin).

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The Effects of Indole-3-Acetic Acid and 2:4-Dichlorophenoxyacetic Acid on the Growth Rate and Endogenous Rhythm of IntactAvenaColeoptiles

Journal of Experimental Botany ◽

10.1093/jxb/7.1.25 ◽

1956 ◽

Vol 7 (1) ◽

pp. 25-41 ◽

Cited By ~ 20

Author(s):

N. G. BALL ◽

INGA J. DYKE

Keyword(s):

Growth Rate ◽

Acetic Acid ◽

Endogenous Rhythm ◽

Dichlorophenoxyacetic Acid ◽

Indole 3 Acetic Acid

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Die Anfangsphasen des durch Steringlucoside und Indol-3-essigsäure stimulierten Wachstums bei Avenakoleoptilen / Initial Phases of Growth Induced by Sterylglucosides and Indole-3-acetic Acid in Avena Coleoptiles

Zeitschrift für Naturforschung C ◽

10.1515/znc-1981-9-1036 ◽

1981 ◽

Vol 36 (9-10) ◽

pp. 900-901 ◽

Cited By ~ 2

Author(s):

Amo Tietz

Keyword(s):

Growth Rate ◽

Acetic Acid ◽

Sharp Increase ◽

Growth Response ◽

Avena Coleoptile ◽

Recording Technique ◽

First Response ◽

Indole 3 Acetic Acid ◽

Primary Action ◽

Membrane Level

AbstractThe initial phases of growth induced by sterylglucosides were investigated by means of a high resolution recording technique. Avena coleoptile segments were preincubated with 10-6 M IAA in buffered solution, then the β-vglucosides of sitosterol, stigmasterol, campesterol, and cholesterol respectively were added in the concentration range from 10-7 –10-6 m . It could be demonstrated that the first response to the applied sterylglucosides is a reduction of the growth rate lasting about 15 min, followed by a sharp increase of the growth rate. As the growth response of the coleoptiles after treatment with sterylglucosides is very similar to that observed after application o f IAA alone, it is concluded from the results that sterylglucosides or sterols may also exhibit their primary action at the membrane level, perhaps by influencing microviscosity of the membrane and/or H +-secretion.

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