The relationship of plasma ADMA levels with cardiac functions and metabolic parameters in peritoneal dialysis patients

To examine the relationship of the formation of biofilm (adherent bacterial microcolonies) to recurrent peritonitis in patients on continuous ambulatory peritoneal dialysis (CAPO) we examined the surfaces of 25 recovered Tenckhoff (T) catheters by scanning electron microscopy (SEM) and microbiological examination of scrapings. Twelve catheters had been removed after successful transplantation; the patients had not been dialyzed for three months and had no evidence of peritonitis during that period (control group), and only seven had experienced peritonitis (I or 2 episodes) before transplantation. All 13 patients in the study group had experienced repeated episodes of peritonitis (range 3–13, average, 5.6). We detected no significant differences between the control and study groups with respect to the percentage of catheter-surface covered by biofilm, or the proportion of catheter segments whose scrapings yielded positive bacterial cultures. It is concluded that large areas of the T catheters of CAPO patients are colonized by bacterial biofilms but only active dialysis predisposes to recurrent peritonitis.

Download Full-text

The relationship between neutrophil-to-lymphocyte ratio and the first occurrence of pneumonia in peritoneal dialysis patients

Clinical and Experimental Nephrology ◽

10.1007/s10157-020-01894-9 ◽

2020 ◽

Vol 24 (9) ◽

pp. 770-778

Author(s):

Yujing Zhang ◽

◽

Xiaochun Lai ◽

Qinkai Chen ◽

Xiaojiang Zhan ◽

...

Keyword(s):

Peritoneal Dialysis ◽

Neutrophil To Lymphocyte Ratio ◽

Dialysis Patients ◽

Lymphocyte Ratio ◽

First Occurrence ◽

The Relationship

Download Full-text

Rate of Synthetic Oligosaccharide Degradation as a Novel Measure of Amylase Activity in Peritoneal Dialysis Patients

Peritoneal Dialysis International ◽

10.1177/089686080802800317 ◽

2008 ◽

Vol 28 (3) ◽

pp. 296-304 ◽

Cited By ~ 2

Author(s):

Elvia García–López ◽

Andrzej Werynski ◽

Olof Heimbürger ◽

José C. Divino Filho ◽

Bengt Lindholm ◽

...

Keyword(s):

Peritoneal Dialysis ◽

Amylase Activity ◽

Ex Vivo ◽

Total Activity ◽

Activity Level ◽

Dialysis Patients ◽

Ex Vivo Model ◽

Patient Groups ◽

Uremic Patients ◽

The Relationship

Background Plasma α–amylase activity is elevated in uremic patients but lower in peritoneal dialysis (PD) patients using icodextrin in comparison to healthy controls. We studied the rate by which an exogenous oligosaccharide (maltoheptaose; G7) is degraded ex vivo by amylase in plasma from PD patients treated with glucose or icodextrin PD solutions. Methods Plasma amylase (pancreatic and total) activity and concentration were measured in 11 controls and in PD patients treated with glucose ( n = 11) and icodextrin ( n = 19). The plasma was spiked with G7 and/or synthetic amylase and the metabolites formed were measured by HPLC following incubation at 37°C for 4 hours. Results The relationship between amylase activity and amylase concentration was similar in all patients and controls. The G7 degradation rate was slower in plasma from icodextrin patients but it was also reduced in patients using glucose compared with the controls, in spite of the higher amylase activity in the glucose group. Normalization (by spiking) of patient plasma with porcine amylase increased but did not normalize the speed of G7 degradation. At a given endogenous amylase activity level, the efficiency of G7 degradation was similar for both patient groups. Conclusions An ex vivo model to study the relationship between amylase activity and the actual rate of carbohydrate (represented by G7) breakdown was developed and showed that PD patients using glucose and icodextrin degrade G7 at a slower speed than controls. This suggests that amylase-mediated carbohydrate metabolism is reduced in PD patients. Further clinical studies are needed to confirm if these findings hold true also in other groups of uremic patients with varying degrees of kidney failure, as well as in patients undergoing hemodialysis.

Download Full-text