Isolation and characterization of the gene encoding a chitin synthase with a myosin motor-like domain from the edible basidiomycetous mushroom, Lentinula edodes, and its expression in the course of fruit-body formation

Mycoscience ◽  
2007 ◽  
Vol 48 (2) ◽  
pp. 109-116 ◽  
Author(s):  
Mikihiro Nishihara ◽  
Akira Watanabe ◽  
Yasuhiko Asada
1997 ◽  
Vol 43 (12) ◽  
pp. 1202-1204 ◽  
Author(s):  
Gab-Gyun Shin ◽  
Sadatoshi Meguro ◽  
Shinsaku Kawachi

This investigation attempted to identify the active constituent in yeast extract for fruit body formation of Lentinula edodes. Yeast extract was applied to a Sephadex G-25 column and was divided into five fractions, I to V, on the basis of the peaks of UV absorption. Of the five fractions, fraction II was the most effective for fruit body formation. Fraction II was still effective for fruit body formation at a dilution of 10−2, but its activity was drastically lowered by autoclaving under alkaline conditions. Thiamin, which is well known to exist in yeast extract and to be sensitive to alkaline hydrolysis, was contained in fraction II in a free form at about 85% of the total content. When most of the thiamin was eliminated by passing it through a permtid column or heating it under alkaline conditions, the fruiting activity of fraction II disappeared. The addition of thiamin, instead of fraction II, also caused fruit body formation in the basal peptone–glucose liquid media. Thus, it was concluded that thiamin must be the active substance in fraction II of the yeast extract for fruit body formation of L. edodes.Key words: Lentinula edodes, fruit body formation, yeast extract, thiamin.


Author(s):  
K. Takaki ◽  
R. Yamaguchi ◽  
N. Yamazaki ◽  
S. Mukaigawa ◽  
T. Fujiwara ◽  
...  

Mycoscience ◽  
2007 ◽  
Vol 48 (2) ◽  
pp. 125-130 ◽  
Author(s):  
Masaru Nagai ◽  
Yuichi Sakamoto ◽  
Keiko Nakade ◽  
Toshitsugu Sato

Mycoscience ◽  
1999 ◽  
Vol 40 (4) ◽  
pp. 349-351 ◽  
Author(s):  
Akihito Kana-uchi ◽  
Takema Fukatsu

Gene ◽  
1996 ◽  
Vol 174 (2) ◽  
pp. 221-224 ◽  
Author(s):  
Travis Henry ◽  
Brian Kliewer ◽  
Robert Palmatier ◽  
Joseph S. Ulphani ◽  
Joe D. Beckmann

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