New method to detect oxolinic acid-resistant Burkholderia glumae infesting rice seeds using a mismatch amplification mutation assay polymerase chain reaction

2004 ◽  
Vol 70 (4) ◽  
Author(s):  
Yukiko Maeda ◽  
Akinori Kiba ◽  
Kouhei Ohnishi ◽  
Yasufumi Hikichi
Keyword(s):  
Polymerase Chain Reaction ◽  
Oxolinic Acid ◽  
New Method ◽  
Chain Reaction ◽  
Burkholderia Glumae ◽  
Polymerase Chain ◽  
Rice Seeds ◽  
Mutation Assay ◽  
Mismatch Amplification Mutation Assay

A New Method for FMR1 Gene Methylation Screening by Multiplex Methylation-Specific Real-Time Polymerase Chain Reaction

2011 ◽  
Vol 15 (6) ◽  
pp. 387-393 ◽  
Author(s):  
Marjanu Hikmah Elias ◽  
Ravindran Ankathil ◽  
Abdul Razak Salmi ◽  
Wanna Sudhikaran ◽  
Pornprot Limprasert ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
New Method ◽  
Fmr1 Gene ◽  
Gene Methylation ◽  
Chain Reaction ◽  
Polymerase Chain

New Method of DNA Isolation from Two Food Additives Suitable for Authentication in Polymerase Chain Reaction Assays

2005 ◽  
Vol 53 (9) ◽  
pp. 3345-3347 ◽  
Author(s):  
Mercedes Urdiaín ◽  
Antonio Doménech-Sánchez ◽  
Sebastián Albertí ◽  
V. Javier Benedí ◽  
Josep A. Rosselló
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Isolation ◽  
Food Additives ◽  
New Method ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals The G209A mutation in the alpha-synuclein gene in Brazilian families with Parkinson's disease

2001 ◽  
Vol 59 (3B) ◽  
pp. 722-724 ◽  
Author(s):  
Hélio A.G. Teive ◽  
Salmo Raskin ◽  
Fábio M. Iwamoto ◽  
Francisco M.B. Germiniani ◽  
Maria H.H. Baran ◽  
...  
Keyword(s):  
Parkinson’S Disease ◽  
Polymerase Chain Reaction ◽  
Parkinson's Disease ◽  
Family History ◽  
Restriction Enzyme ◽  
Alpha Synuclein ◽  
Chain Reaction ◽  
The Mean ◽  
Polymerase Chain ◽  
Mutation Assay

A missense G209A mutation of the alpha-synuclein gene was recently described in a large Contursi kindred with Parkinson's disease (PD). The objective of this study is to determine if the mutation G209A of the alpha-synuclein gene was present in 10 Brazilian families with PD. PD patients were recruited from movement disorders clinics of Brazil. A family history with two or more affected in relatives was the inclusion criterion for this study. The alpha-synuclein G209A mutation assay was made using polymerase chain reaction and the restriction enzyme Tsp45I. Ten patients from 10 unrelated families were studied. The mean age of PD onset was 42.7 years old. We did not find the G209A mutation in our 10 families with PD. Our results suggest that alpha-synuclein mutation G209A is uncommon in Brazilian PD families.

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