Molecular analysis and virus transmission tests place Olpidium virulentus, a vector of Mirafiori lettuce big-vein virus and tobacco stunt virus, as a distinct species rather than a strain of Olpidium brassicae

2006 ◽  
Vol 72 (1) ◽  
pp. 20-25 ◽  
Author(s):  
Takahide Sasaya ◽  
Hiroki Koganezawa
Keyword(s):  
Molecular Analysis ◽  
Virus Transmission ◽  
Distinct Species ◽  
Olpidium Virulentus ◽  
Olpidium Brassicae

scholarly journals Selecting a Genetic Region for Molecular Analysis of Hepatitis B Virus Transmission

2007 ◽  
Vol 45 (2) ◽  
pp. 687-689 ◽  
Author(s):  
S. Datta ◽  
A. Banerjee ◽  
P. K. Chandra ◽  
R. Chakravarty ◽  
B. C. Cowie
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Molecular Analysis ◽  
Virus Transmission ◽  
Genetic Region ◽  
B Virus

scholarly journals Detection of Badnavirus in pineapple in northeastern Brazil

2020 ◽  
Vol 5 (4) ◽  
pp. 2450-2463
Author(s):  
Edlene Maria da Silva Moraes Santos ◽  
Jaqueline Figueredo de Oliveira Costa ◽  
Mayra Machado de Medeiros Ferro ◽  
Sarah Jacqueline Cavalcanti da Silva ◽  
Iraildes Pereira Assunção ◽  
...  
Keyword(s):  
Dna Extraction ◽  
Molecular Analysis ◽  
Distinct Species ◽  
Northeastern Brazil ◽  
Ananas Comosus ◽  
Pairwise Comparisons ◽  
Sequencing Analysis ◽  
First Report ◽  
Northeastern Region ◽  
Total Dna

ABSTRACT: Brazil is one of the main global producers of pineapple (Ananas comosus L. Merril), with emphasis in the Northeastern region of the country. Pineapple is exposed to several phytosanitary problems, including viruses. Pineapple badnaviruses are caused by two distinct species: Pineapple bacilliform CO virus (PBCoV) and Pineapple bacilliform ER virus (PBErV). The present study aimed to detect possible species of the genus Badnavirus in pineapple samples in Northeastern Brazil, via PCR and sequencing of the RT/RNaseH region. Leaf samples of pineapples were collected in the states of Alagoas, Maranhão, Paraiba and Pernambuco, and subsequently subjected to total DNA extraction and amplification via PCR. Badnavirus positive samples were selected for sequencing. Analysis of pairwise comparisons revealed that all sequences obtained in this work showed an identity greater than 80% with the sequence of the species PBCoV (EU377664), from Australia, fully corroborating with phylogenetic analyzes. These results suggest the widespread of PBCoV in Northeastern Brazil and record the first report of Badnavirus in pineapple culture in Brazil. KEYWORDS: Caulimoviridae, Ananas comosus, molecular analysis.

Pristipomoides amoenus (Snyder 1911), a valid species of jobfish (Pisces, Lutjanidae), with comparisons to P. argyrogrammicus (Valenciennes 1832)

Zootaxa ◽  
2020 ◽  
Vol 4728 (4) ◽  
pp. 469-476
Author(s):  
TAMAKI SHIMOSE ◽  
NOBUAKI SUZUKI ◽  
YUKIO IWATSUKI
Keyword(s):  
Molecular Analysis ◽  
Distinct Species ◽  
Genetic Differences ◽  
Coi Gene ◽  
Valid Species ◽  
Color Patterns ◽  
Type Specimens ◽  
Gill Rakers ◽  
Ishigaki Island ◽  
Mtdna Coi

Platyinius amoenus Snyder 1911 has long been considered a junior synonym of Pristipomoides argyrogrammicus (Valenciennes 1832), however, the two are discernible as distinct species from color patterns and counts of gill rakers based on examination of their type materials and non-type specimens. Furthermore, genetic differences based on molecular analysis among the two species (mtDNA, COI gene) strongly support the validity of both species. Pristipomoides amoenus can be readily distinguished from P. argyrogrammicus in having no white margin to the dorsal, anal, and caudal fins, silvery blue spots continuing inside dorso-lateral yellow blotches, and presence of modally10 (vs. modally 12) lower gill rakers. Both species were collected sympatrically around Ishigaki Island, Okinawa, Japan. 

scholarly journals Nesticus kosodensis Yaginuma, 1972 bona species. Molecular and morphological separation from N. latiscapus Yaginuma, 1972 with notes on cave scaffold-web spiders subspecies in Japan (Araneae, Nesticidae)

2020 ◽  
Vol 35 ◽  
pp. 79-96
Author(s):  
Yuya Suzuki ◽  
Francesco Ballarin
Keyword(s):  
Molecular Analysis ◽  
Taxonomic Revision ◽  
Distinct Species ◽  
Species Level ◽  
Nominal Species ◽  
Cave Fauna ◽  
The Status ◽  
Taxonomic Approach

The troglophilic spider subspecies Nesticus latiscapus kosodensis Yaginuma, 1972 is compared with its nominal species N. latiscapus Yaginuma, 1972 using an integrated taxonomic approach. Both morphological and molecular analysis suggest the two taxa are distinct, rejecting the status of subspecies. In the light of these results, N. kosodensisstat. nov. is elevated to species level and both species are redescribed. We expect that future revisions of Japanese Nesticidae will find that many currently designated subspecies are in fact distinct species. Taxonomic revision of long-established and unrevised subspecies may help to improve the conservation efforts aiming to protect the Japanese endemic cave fauna.

scholarly journals Evidence of olive mild mosaic virus transmission by Olpidium brassicae

2011 ◽  
Vol 130 (2) ◽  
pp. 165-172 ◽  
Author(s):  
Carla M. R. Varanda ◽  
Marta S. M. R. Silva ◽  
Maria do Rosário F. Félix ◽  
Maria Ivone E. Clara
Keyword(s):  
Mosaic Virus ◽  
Virus Transmission ◽  
Mild Mosaic ◽  
Mild Mosaic Virus ◽  
Olpidium Brassicae

scholarly journals Evaluation of Procedures for the Collection, Processing, and Analysis of Biomolecules from Low-Biomass Surfaces

2011 ◽  
Vol 77 (9) ◽  
pp. 2943-2953 ◽  
Author(s):  
K. Kwan ◽  
M. Cooper ◽  
M. T. La Duc ◽  
P. Vaishampayan ◽  
C. Stam ◽  
...  
Keyword(s):  
Molecular Analysis ◽  
Distinct Species ◽  
Pcr Analysis ◽  
Rrna Gene ◽  
Sample Collection ◽  
Sampling Device ◽  
Small Surface ◽  
Cellular Density ◽  
Specific Pcr ◽  
Species Specific

ABSTRACTTo comprehensively assess microbial diversity and abundance via molecular-analysis-based methods, procedures for sample collection, processing, and analysis were evaluated in depth. A model microbial community (MMC) of known composition, representative of a typical low-biomass surface sample, was used to examine the effects of variables in sampling matrices, target cell density/molecule concentration, and cryogenic storage on the overall efficacy of the sampling regimen. The MMC used in this study comprised 11 distinct species of bacterial, archaeal, and fungal lineages associated with either spacecraft or clean-room surfaces. A known cellular density of MMC was deposited onto stainless steel coupons, and after drying, a variety of sampling devices were used to recover cells and biomolecules. The biomolecules and cells/spores recovered from each collection device were assessed by cultivable and microscopic enumeration, and quantitative and species-specific PCR assays. rRNA gene-based quantitative PCR analysis showed that cotton swabs were superior to nylon-flocked swabs for sampling of small surface areas, and for larger surfaces, biological sampling kits significantly outperformed polyester wipes. Species-specific PCR revealed differential recovery of certain species dependent upon the sampling device employed. The results of this study empower current and future molecular-analysis-based microbial sampling and processing methodologies.

Caloplaca fuscorufa—a misunderstood species in northern Europe

2007 ◽  
Vol 39 (5) ◽  
pp. 409-414 ◽  
Author(s):  
Ulf ARUP ◽  
Emma ARNENG ◽  
Ulrik SØCHTING
Keyword(s):  
Molecular Analysis ◽  
Related Species ◽  
Distinct Species ◽  
Molecular Data ◽  
Chemical Data ◽  
Northern Europe ◽  
Northern Sweden ◽  
Northern Distribution ◽  
Long Time ◽  
Single Locality

Abstract:Caloplaca fuscorufa H. Magn. is a poorly known species that for a long time was known only from a single locality in Jämtland, northern Sweden, where it was collected in 1910. It has been suggested to be synonymous with C. crenularia (With.) J. R. Laundon, but has also been confused with C. exsecuta (Nyl.) Dalla Torre & Sarnth. We have compared the isotype of C. fuscorufa and material collected in Sweden, Norway and Svalbard, with presumably related species, using morphology, anatomy and chemistry as well as molecular data. The results show that C. fuscorufa is a distinct species, differing from C. crenularia mainly in its somewhat larger spores with wider septa, different colour of the apothecia and a more northern distribution. It differs from C. exsecuta mainly in its chemistry and smaller spores with smaller septa. Caloplaca crenularia is shown to have a more southern distribution in Sweden than previously understood. Chemical data and a molecular analysis based on ITS data show that C. fuscorufa is related to C. crenularia and C. ammiospila, but clearly separated from these species.

scholarly journals Olive mild mosaic virus transmission by Olpidium virulentus

2015 ◽  
Vol 142 (1) ◽  
pp. 197-201 ◽  
Author(s):  
Carla M. R. Varanda ◽  
Susana Santos ◽  
Maria Ivone E. Clara ◽  
Maria do Rosário Félix
Keyword(s):  
Mosaic Virus ◽  
Virus Transmission ◽  
Mild Mosaic ◽  
Olpidium Virulentus ◽  
Mild Mosaic Virus
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