Identification and characterization of Colletotrichum gloeosporioides sensu stricto, the agent of anthracnose disease of Sorbaria sorbifolia in China

Author(s):  
Qing-Hai Wang ◽  
Xiang-Ying Li ◽  
Ting-Ting Xu ◽  
Meng-Ai Zhang ◽  
Yu-Hui Yang ◽  
...  
2006 ◽  
Vol 96 (5) ◽  
pp. 542-548 ◽  
Author(s):  
Marcel Maymon ◽  
Aida Zveibil ◽  
Shimon Pivonia ◽  
Dror Minz ◽  
Stanley Freeman

Sixty-four isolates of Colletotrichum gloeosporioides were isolated from infected Limonium spp. cultivated in 12 different locations in Israel. All isolates were identified as belonging to the C. gloeosporioides complex by species-specific primers. Of these isolates, 46 were resistant to benomyl at 10 μg/ml and 18 were sensitive to this concentration of fungicide. Based on arbitrarily primed polymerase chain reaction of all isolates and internal transcribed spacer-1 sequence analyses of 12 selected isolates, the benomyl-resistant and -sensitive populations belong to two distinct genotypes. Sequence analyses of the β-tubulin genes, TUB1 and TUB2, of five sensitive and five resistant representative isolates of C. gloeosporioides from Limonium spp. revealed that the benomyl-resistant isolates had an alanine substitute instead of a glutamic acid at position 198 in TUB2. All data suggest that the resistant and sensitive genotypes are two independent and separate populations. Because all Limonium plant propagation material is imported from various geographic regions worldwide, and benomyl is not applied to this crop or for the control of Colletotrichum spp. in Israel, it is presumed that plants are bearing quiescent infections from the points of origin prior to arrival.


2018 ◽  
Vol 210 ◽  
pp. 26-32 ◽  
Author(s):  
Lizeth Guardado-Valdivia ◽  
Erik Tovar-Pérez ◽  
Alejandra Chacón-López ◽  
Ulises López-García ◽  
Porfirio Gutiérrez-Martínez ◽  
...  

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