Compound-Specific Isotope Analysis of Amino Acid Labeling with Stable Isotope Nitrogen (15N) in Higher Plants

2016 ◽  
Vol 79 (17-18) ◽  
pp. 1197-1205 ◽  
Author(s):  
Zhongyi Zhang ◽  
Huayun Xiao ◽  
Nengjian Zheng ◽  
Xiaofei Gao ◽  
RenGuo Zhu
Keyword(s):  
Amino Acid ◽  
Stable Isotope ◽  
Higher Plants ◽  
Isotope Analysis ◽  
Compound Specific Isotope Analysis ◽  
Amino Acid Labeling

Land‐use change erodes trophic redundancy in tropical forest streams: evidence from amino acid stable isotope analysis

2021 ◽  
Author(s):  
Kenny W.J. Chua ◽  
Jia Huan Liew ◽  
Clare L. Wilkinson ◽  
Amirrudin B. Ahmad ◽  
Heok Hui Tan ◽  
...  
Keyword(s):  
Land Use ◽  
Amino Acid ◽  
Stable Isotope ◽  
Land Use Change ◽  
Tropical Forest ◽  
Stable Isotope Analysis ◽  
Isotope Analysis ◽  
Forest Streams ◽  
Acid Stable

Multi-element (C, H, Cl, Br) stable isotope fractionation as a tool to investigate transformation processes for halogenated hydrocarbons

2020 ◽  
Vol 22 (3) ◽  
pp. 567-582
Author(s):  
Ann Sullivan Ojeda ◽  
Elizabeth Phillips ◽  
Barbara Sherwood Lollar
Keyword(s):  
Stable Isotope ◽  
Isotope Fractionation ◽  
Isotope Analysis ◽  
Halogenated Hydrocarbons ◽  
Halogenated Hydrocarbon ◽  
Stable Isotope Fractionation ◽  
Compound Specific Isotope Analysis ◽  
Transformation Processes

A review that highlights the utility of multi-element compound-specific isotope analysis (CSIA) in halogenated hydrocarbon remediation.

scholarly journals Stable nitrogen isotope analysis of amino acids as a new tool to clarify complex parasite-host interactions within marine food webs

2021 ◽  
Author(s):  
Philip Riekenberg ◽  
Tijs Joling ◽  
Lonneke L. IJsseldijk ◽  
Andreas M. Waser ◽  
Marcel van der Meer ◽  
...  
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Stable Isotope ◽  
Food Webs ◽  
Stable Isotope Analysis ◽  
Trophic Interactions ◽  
Trophic Position ◽  
Isotope Analysis ◽  
Isotopic Analysis ◽  
Parasitic Interactions

AbstractTraditional bulk isotopic analysis is a pivotal tool for mapping consumer-resource interactions in food webs but has largely failed to adequately describe parasite-host relationships. Thus, parasite-host interactions remain largely understudied in food web frameworks despite these relationships increasing linkage density, connectance, and ecosystem biomass. Compound-specific stable isotopes from amino acids provides a promising novel approach that may aid in mapping parasitic interactions in food webs. However, to date it has not been applied to parasitic trophic interactions.Here we use a combination of traditional bulk stable isotope analyses and compound-specific isotopic analysis of the nitrogen in amino acids to examine resource use and trophic interactions of five parasites from three hosts from a marine coastal food web (Wadden Sea, European Atlantic). By comparing isotopic compositions of bulk and amino acid nitrogen, we aimed to characterize isotopic fractionation occurring between parasites and their hosts and to clarify the trophic position of the parasites.Our results showed that parasitic trophic interactions were more accurately identified when using compound-specific stable isotope analysis due to removal of underlying source isotopic variation for both parasites and hosts, and avoidance of the averaging of amino acid variability in bulk analyses through use of multiple trophic amino acids. The compound-specific method provided clear trophic discrimination factors in comparison to bulk isotope methods, however, those differences varied significantly among parasite species.Amino acid compound specific isotope analysis has widely been applied to examine trophic position within food webs, but our analyses suggest that the method is particularly useful for clarifying the feeding strategies for parasitic species. Baseline isotopic information provided by source amino acids allows clear identification of the fractionation occurring due to parasite metabolism by integrating underlying isotopic variations from the host tissues. However, like for bulk isotope analysis, the application of a universal trophic discrimination factor to parasite-host relationships remains inappropriate for compound-specific stable isotope analysis. Despite this limitation, compound-specific stable isotope analysis is and will continue to be a valuable tool to increase our understanding of parasitic interactions in marine food webs.

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