Wenzhouxiangella marina gen. nov, sp. nov, a marine bacterium from the culture broth of Picochlorum sp. 122, and proposal of Wenzhouxiangellaceae fam. nov. in the order Chromatiales

2015 ◽  
Vol 107 (6) ◽  
pp. 1625-1632 ◽  
Author(s):  
Guanghua Wang ◽  
Mingxing Tang ◽  
Tao Li ◽  
Shikun Dai ◽  
Huanlian Wu ◽  
...  
Keyword(s):  
Marine Bacterium ◽  
Culture Broth

scholarly journals ISOLATION OF ACTINOMYCIN D FROM MARINE DERIVED Streptomyces

2010 ◽  
Vol 7 (3) ◽  
pp. 350-353
Author(s):  
Abdullah Rasyid ◽  
Kyoko Adachi
Keyword(s):  
Antibacterial Activity ◽  
Crude Extract ◽  
Marine Bacterium ◽  
Actinomycin D ◽  
Culture Supernatant ◽  
Culture Broth ◽  
Bacterial Cells ◽  
Antibacterial Screening ◽  
Activity Test ◽  
Bioactive Substance

The bioactive substance producing strain, a marine bacterium A5S-46 was found in the antibacterial screening. This strain was isolated from the artificial sponge set in the coastal seawater at Iriomote islands (Japan). The crude extract from culture broth of the strain A5S-46 was assayed to the antibacterial activity test against the seven kinds of bacterial test strains. The active substance accumulated in the both bacterial cells and culture supernatant. Based on the 1H NMR and the LC/PDA/MS data, the bioactive substance was identified to be actinomycin D.   Keywords: Marine bacteria, Actinomycin D, Antibacterial, Anticancer

scholarly journals Saccharobisindole, Neoasterric Methyl Ester, and 7-Chloro-4(1H)-quinolone: Three New Compounds Isolated from the Marine Bacterium Saccharomonospora sp.

Marine Drugs ◽  
2021 ◽  
Vol 20 (1) ◽  
pp. 35
Author(s):  
Sohee Kim ◽  
Tu Cam Le ◽  
Sang-Ah Han ◽  
Prima F. Hillman ◽  
Ahreum Hong ◽  
...  
Keyword(s):  
Methyl Ester ◽  
Marine Bacterium ◽  
High Resolution Mass Spectrometry ◽  
Micrococcus Luteus ◽  
Culture Broth ◽  
Chemical Components ◽  
Inhibition Activity ◽  
Chemical Structures ◽  
New Compounds ◽  
And Staphylococcus Aureus

Analysis of the chemical components from the culture broth of the marine bacterium Saccharomonospora sp. CNQ-490 has yielded three novel compounds: saccharobisindole (1), neoasterric methyl ester (2), and 7-chloro-4(1H)-quinolone (3), in addition to acremonidine E (4), pinselin (5), penicitrinon A (6), and penicitrinon E (7). The chemical structures of the three novel compounds were elucidated by the interpretation of 1D, 2D nuclear magnetic resonance (NMR), and high-resolution mass spectrometry (HRMS) data. Compound 2 generated weak inhibition activity against Bacillus subtilis KCTC2441 and Staphylococcus aureus KCTC1927 at concentrations of 32 μg/mL and 64 μg/mL, respectively, whereas compounds 1 and 3 did not have any observable effects. In addition, compound 2 displayed weak anti-quorum sensing (QS) effects against S. aureus KCTC1927 and Micrococcus luteus SCO560.

scholarly journals Two new 2-alkylquinolones, inhibitory to the fish skin ulcer pathogen Tenacibaculum maritimum, produced by a rhizobacterium of the genus Burkholderia sp.

2018 ◽  
Vol 14 ◽  
pp. 1446-1451 ◽  
Author(s):  
Dandan Li ◽  
Naoya Oku ◽  
Atsumi Hasada ◽  
Masafumi Shimizu ◽  
Yasuhiro Igarashi
Keyword(s):  
Mass Spectrometry ◽  
Nmr Spectroscopy ◽  
Marine Fish ◽  
Marine Bacterium ◽  
Fish Farming ◽  
Culture Broth ◽  
Skin Ulcer ◽  
Bioactive Molecules ◽  
Fish Skin ◽  
Skin Ulcers

Exploration of rhizobacteria of the genus Burkholderia as an under-tapped resource of bioactive molecules resulted in the isolation of two new antimicrobial 2-alkyl-4-quinolones. (E)-2-(Hept-2-en-1-yl)quinolin-4(1H)-one (1) and (E)-2-(non-2-en-1-yl)quinolin-4(1H)-one (3) were isolated from the culture broth of strain MBAF1239 together with four known alkylquinolones (2 and 4–6), pyrrolnitrin (7), and BN-227 (8). The structures of 1 and 3 were unambiguously characterized using NMR spectroscopy and mass spectrometry. Compounds 1–8 inhibited the growth of the marine bacterium Tenacibaculum maritimum, an etiological agent of skin ulcers in marine fish, offering new opportunities to develop antibacterial drugs for fish farming.

scholarly journals Pelagibacterium lentulum sp. nov., a marine bacterium from the culture broth of Picochlorum sp. 122

2017 ◽  
Vol 67 (9) ◽  
pp. 3182-3185 ◽  
Author(s):  
Guanghua Wang ◽  
Kefu Yu ◽  
Yinghui Wang ◽  
Hongfei Su ◽  
Hualian Wu ◽  
...  
Keyword(s):  
Marine Bacterium ◽  
Culture Broth

scholarly journals Histidinibacterium lentulum gen. nov., sp. nov., a marine bacterium from the culture broth of marine microalga Picochlorum sp. 122

2019 ◽  
Vol 69 (2) ◽  
pp. 578-583 ◽  
Author(s):  
Guanghua Wang ◽  
Shuailiang Xu ◽  
Hongfei Su ◽  
Biao Chen ◽  
Jiayuan Liang ◽  
...  
Keyword(s):  
Marine Bacterium ◽  
Culture Broth ◽  
Marine Microalga

scholarly journals Pyruvatibacter mobilis gen. nov., sp. nov., a marine bacterium from the culture broth of Picochlorum sp. 122

2016 ◽  
Vol 66 (1) ◽  
pp. 184-188 ◽  
Author(s):  
Guanghua Wang ◽  
Mingxing Tang ◽  
Hualian Wu ◽  
Shikun Dai ◽  
Tao Li ◽  
...  
Keyword(s):  
Marine Bacterium ◽  
Culture Broth

scholarly journals Butyratibacter algicola gen. nov., sp. nov., a marine bacterium from the culture broth of Picochlorum sp. 122

2017 ◽  
Vol 67 (9) ◽  
pp. 3209-3213 ◽  
Author(s):  
Guanghua Wang ◽  
Yinghui Wang ◽  
Hongfei Su ◽  
Hualian Wu ◽  
Tao Li ◽  
...  
Keyword(s):  
Marine Bacterium ◽  
Culture Broth

scholarly journals Marine Bacterium Vibrio sp. CB1-14 Produces Guanidine Alkaloid 6-epi-Monanchorin, Previously Isolated from Marine Polychaete and Sponges

Marine Drugs ◽  
2019 ◽  
Vol 17 (4) ◽  
pp. 213 ◽  
Author(s):  
Tatyana Makarieva ◽  
Larisa Shubina ◽  
Valeria Kurilenko ◽  
Marina Isaeva ◽  
Nadezhda Chernysheva ◽  
...  
Keyword(s):  
New Species ◽  
Marine Bacterium ◽  
Culture Broth ◽  
Rrna Gene ◽  
Bacterial Strains ◽  
Genome Sequences ◽  
16S Rrna Gene Analysis ◽  
Chaetopterus Variopedatus ◽  
Marine Polychaete ◽  
A New Species

Twenty-three bacterial strains were isolated from the secreted mucus trapping net of themarine polychaete Chaetopterus variopedatus (phylum Annelida) and twenty strains were identifiedusing 16S rRNA gene analysis. Strain CB1-14 was recognized as a new species of the genus Vibriousing the eight-gene multilocus sequence analysis (MLSA) and genome sequences of nineteen typeVibrio strains. This Vibrio sp. was cultured, and 6-epi-monanchorin (2), previously isolated from thepolychaete and two sponge species, was found in the cells and culture broth. The presence of the 6-epi-monanchorin was confirmed by its isolation followed by 1H NMR and HRESIMS analysis. Theseresults showed the microbial origin of the bicyclic guanidine alkaloid 2 in C. variopedatus.

scholarly journals Molecular Characterization of an Endo-β-1,4-Glucanase, CelAJ93, from the Recently Isolated Marine Bacterium, Cellulophaga sp. J9-3

2019 ◽  
Vol 9 (19) ◽  
pp. 4061
Author(s):  
Da Som Kim ◽  
Won-Jae Chi ◽  
Soon-Kwang Hong
Keyword(s):  
Amino Acids ◽  
Marine Bacterium ◽  
Ethylenediaminetetraacetic Acid ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Apparent Molecular Weight ◽  
Culture Broth ◽  
Rrna Gene ◽  
Glycosyl Hydrolases ◽  
Sequencing Data

A novel cellulase was characterized from a newly isolated marine bacterium, strain J9-3. Phylogenetic analysis based on the 16S rRNA gene revealed that strain J9-3 belonged to the genus Cellulophaga, and thus, it was named Cellulophaga sp. J9-3. An extracellular cellulase was purified from cell-free culture broth of J9-3 cultured in Marine Broth containing 0.2% carboxymethylcellulose. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the protein revealed a single band with an apparent molecular weight (Mw) of 35 kDa. Based on the NH2-terminal amino acid sequence (N-N-T-E-Q-T-V-V-D-A-Y-G), the gene (named celAJ93) encoding the protein was identified from J9-3 genomic sequencing data. CelAJ93 was expected to be translated into a premature protein (359 amino acids) and then processed to a mature protein (307 amino acids, Mw = 34,951 Da), which is consistent with our results. CelAJ93 had high homology with many uncharacterized putative glycosyl hydrolases of the genus Cellulophaga and it was highly specific for carboxymethylcellulose and cellooligosaccharides under optimum conditions (pH 7.5, 60 °C). Co2+ completely recovered CelAJ93 activity that was severely inhibited by ethylenediaminetetraacetic acid (EDTA), indicating that CelAJ93 required Co2+ as a cofactor. Thus, CelAJ93 is a Co2+-dependent endo-β-1,4-glucanase that can hydrolyze carboxymethylcellulose and cellooligosaccharides into cellobiose at a relatively high temperature.

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