Estimation of Isotonic Point of Incubation Medium For Two-Cell Mouse Embryo

2011 ◽  
Vol 152 (1) ◽  
pp. 142-145 ◽  
Author(s):  
M. A. Pogorelova ◽  
V. A. Golichenkov ◽  
V. N. Pogorelova ◽  
E. V. Kornienko ◽  
A. I. Panait ◽  
...  
Keyword(s):  
Mouse Embryo ◽  
Incubation Medium ◽  
Cell Mouse Embryo

Reporter Gene Expression in G2 of the 1-Cell Mouse Embryo

1993 ◽  
Vol 156 (2) ◽  
pp. 552-556 ◽  
Author(s):  
Prahlad T. Ram ◽  
Richard M. Schultz
Keyword(s):  
Gene Expression ◽  
Reporter Gene ◽  
Mouse Embryo ◽  
Reporter Gene Expression ◽  
Cell Mouse Embryo

Evidence for expression of the paternal genome in the two-cell mouse embryo

Nature ◽  
1981 ◽  
Vol 294 (5840) ◽  
pp. 450-451 ◽  
Author(s):  
Janet A. Sawicki ◽  
Terry Magnuson ◽  
Charles J. Epstein
Keyword(s):  
Mouse Embryo ◽  
Paternal Genome ◽  
Cell Mouse Embryo

scholarly journals Long-Lived Binding of Sox2 to DNA Predicts Cell Fate in the Four-Cell Mouse Embryo

Cell ◽  
2016 ◽  
Vol 165 (1) ◽  
pp. 75-87 ◽  
Author(s):  
Melanie D. White ◽  
Juan F. Angiolini ◽  
Yanina D. Alvarez ◽  
Gurpreet Kaur ◽  
Ziqing W. Zhao ◽  
...  
Keyword(s):  
Cell Fate ◽  
Mouse Embryo ◽  
Cell Mouse Embryo

Apparent absence of antiport activity in the two-cell mouse embryo

1990 ◽  
Vol 138 (2) ◽  
pp. 421-429 ◽  
Author(s):  
Jay M. Baltz ◽  
John D. Biggers ◽  
Claude Lechene
Keyword(s):  
Mouse Embryo ◽  
Apparent Absence ◽  
Cell Mouse Embryo

The distribution of ingested horseradish peroxidase in the 16-cell mouse embryo

Development ◽  
1981 ◽  
Vol 66 (1) ◽  
pp. 191-207
Author(s):  
W. J. D. Reeve
Keyword(s):  
Horseradish Peroxidase ◽  
Mouse Embryo ◽  
Single Cells ◽  
Fluorescent Antibody ◽  
Surface Binding ◽  
Cytoplasmic Organization ◽  
Cell Embryo ◽  
Polarized Surface ◽  
Cell Mouse Embryo

Cells of the 16-cell mouse embryo endocytose horseradish peroxidase (HRP) which becomes localized in most cases to a juxtanuclear position. Cells that have ingested HRP in intact embryos, and cells dissociated from embryos prior to culture in HRP, showed similar patterns of cytoplasmic distribution of the ingested enzyme. Cells in the embryo in situ were incubated in HRP, and then labelled with fluorescent antibody either before (to label the outside surface of the embryo) or after (to reveal populations of outer polar and inner apolar cells) their disaggregation into single cells. The population of polar outside cells from the morula includes more cells with a highly restricted localization of HRPcontaining vesicles than does the population of inside cells, and this restricted localization underlies the exposed surface or pole of the cell. A 2/16 couplet formed by division in vitro of a 1/8 cell is comparable to the pairs of cells dissociated from 16-cell embryos; most couplets from either source consisted of a larger cell that showed polarized surface binding of fluorescent ligand (fluorescent pole) and a smaller cell with a uniform distribution of bound ligand. The incidence of restricted patterns of HRP staining was highest among populations of both larger and polar cells. When 1/8 cells labelled with HRP are observed during division to 2/16, the previously clustered vesicles of ingested HRP become more dispersed throughout the cytoplasm and, although the two cells of some couplets can stain differently very soon after their formation, the patterns of distribution of HRP take about 1 h after division to stabilize. These observations are consistent with cells of the 16-cell embryo inheriting different features of cytoplasmic organization.

The temporal effects of changes in in vitro fertilization culture media on the one-cell mouse embryo system

1988 ◽  
Vol 5 (3) ◽  
pp. 149-152 ◽  
Author(s):  
Ady Davidson ◽  
Michael Vermesh ◽  
Rogerio A. Lobo ◽  
Richard J. Paulson
Keyword(s):  
In Vitro Fertilization ◽  
Mouse Embryo ◽  
Culture Media ◽  
Vitro Fertilization ◽  
Temporal Effects ◽  
The One ◽  
Cell Mouse Embryo

298. Dietary protein does not influence mitochondrial distribution in the 2-cell mouse embryo

2005 ◽  
Vol 17 (9) ◽  
pp. 126
Author(s):  
M. Mitchell ◽  
M. Lane
Keyword(s):  
Mouse Embryo ◽  
Dietary Protein ◽  
Molecular Probes ◽  
Cell Cortex ◽  
Mitochondrial Activity ◽  
Cortical Region ◽  
Embryo Viability ◽  
Mitochondrial Distribution ◽  
Cell Mouse Embryo

Excess dietary protein can negatively influence fertility. The underlying mechanisms remain to be completely elucidated; however, variations in reproductive tract pH, and ammonium and urea concentrations have been implicated. Mouse embryos cultured in the presence of ammonium showed a shift in mitochondrial distribution away from the nucleus towards the cell cortex, suggestive of reduced mitochondrial activity, ATP production and embryo viability. In this study we determined the effect of dietary protein in vivo, on mitochondrial distribution in the 2-cell mouse embryo. Five-week-old Swiss female mice (n = 10) were fed low (9%), medium (14%) or high (25%) dietary protein for 3weeks; feed intake and body weight were recorded weekly. At 8 weeks of age mice were primed with 5 IU of PMSG, then 5 IU hCG 48 h later, and mated overnight with males of proven fertility. Forty hours post-hCG females were sacrificed, their oviducts collected and flushed with media. The total number of 2-cell embryos and oocytes retrieved were recorded. Active mitochondria were stained in the 2-cell embryos using Mitotracker Green (Molecular Probes), and were visualised using confocal microscopy. Density of perinuclear and cortical staining was determined in Photoshop 7.0, using an established method. Females fed the medium diet consumed significantly less and gained less weight than those fed the low or high diet (Table 1), despite similar final body weights (data not shown). Females fed the low diet tended to have a lower ovulation rate and fewer 2-cell embryos than females consuming the other diets (Table 1, P > 0.05). There was no significant effect of dietary protein on the distribution of mitochondria between the perinuclear and cortical region of the embryo, which may be reflective of lower in vivo ammonium levels compared to those described in culture.

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