Monooxygenase System and NO Metabolism in Liver Microsomes of Rats with Toxic Hepatitis and the Effect of Sesquiterpene Lactones

2021 ◽  
Vol 172 (2) ◽  
pp. 133-136
Author(s):  
N. V. Tursunova ◽  
V. N. Syrov ◽  
Z. A. Khushbaktova ◽  
Yu. V. Tornuev ◽  
M. G. Klinnikova
Keyword(s):  
Toxic Hepatitis ◽  
Sesquiterpene Lactones ◽  
Liver Microsomes ◽  
Monooxygenase System

Cytochrome P-450-linked monooxygenase system and drug-induced spectral interactions in human liver microsomes

1974 ◽  
Vol 9 (3) ◽  
pp. 205-216 ◽  
Author(s):  
Olavi Pelkonen ◽  
Eero H. Kaltiala ◽  
Teuvo K.I. Larmi ◽  
Niilo T. Kärki
Keyword(s):  
Human Liver ◽  
Liver Microsomes ◽  
Human Liver Microsomes ◽  
Monooxygenase System ◽  
Drug Induced ◽  
Cytochrome P 450

A Direct, Highly Sensitive Fluorometric Assay for a Microsomal Cytochrome P450-Mediated O-Demethylation Using a Novel Coumarin Analog as Substrate

2000 ◽  
Vol 55 (11-12) ◽  
pp. 915-922 ◽  
Author(s):  
René Roser ◽  
Helmut Thomas
Keyword(s):  
Rat Liver ◽  
Liver Microsomes ◽  
Fluorometric Assay ◽  
Excitation Wavelength ◽  
Monooxygenase System ◽  
Rat Liver Microsomes ◽  
Monooxygenase Activity ◽  
Continuous Increase ◽  
Highly Sensitive

A highly sensitive fluorometric assay for the determination of monooxygenase activity in liver microsomes is described. The assay is based on the use of 3-chloro-7-methoxy-4-methylcoumarin which is demethylated to 3-chloro-7-hydroxy-4-methylcoumarin. The rate of formation of 3-chloro-7-hydroxy-4-methylcoumarin was recorded as an increase of fluorescence (λA = 380 nm, λF = 480 nm ) with time. When 3-chloro-7-methoxy-4-methylcoumarin was incubated in the presence of MgCl2and NADPH with rat liver microsomes, a continuous increase of the fluorescence could be measured. The reaction proceeded linearly for about 10 min and at least up to a concentration of 0.1 mg/ml of microsomal protein. Besides 3-chloro-7-hydroxy-4-methylcoumarin a hydroxylated derivative of the substrate was formed as a second metabolite during the incubation. Using an excitation wavelength of 480 nm and a fluorescence/em ission w avelength of 480 nm, the fluorescence of this substance (λA = 338 nm, λF = 422 nm ) amounted only to about 1% of the fluorescence of the main product. The use of 3-chloro-7-methoxy-4-methylcoumarin as substrate enables the fluorometric determination of the O-dealkylation activity of a cytochrom e P450-dependent monooxygenase system in rat liver which is inducible by phenobarbital but not by 3-methylcholanthrene.

Effect of n-hexane inhalation on the monooxygenase system in MICE liver microsomes

1974 ◽  
Vol 8 (1) ◽  
pp. 11-18 ◽  
Author(s):  
A. Krämer ◽  
Hj. Staudinger ◽  
V. Ullrich
Keyword(s):  
Liver Microsomes ◽  
Monooxygenase System ◽  
Mice Liver

The presence of a monooxygenase system in human fetal liver microsomes

Life Sciences ◽  
1970 ◽  
Vol 9 (20) ◽  
pp. 1189-1200 ◽  
Author(s):  
S.J. Yaffe ◽  
A. Rane ◽  
F. Sjöqvist ◽  
L.-O. Boréus ◽  
S. Orrenius
Keyword(s):  
Fetal Liver ◽  
Liver Microsomes ◽  
Monooxygenase System ◽  
Human Fetal Liver

Sex difference in responsiveness to aztreonam of monooxygenase system in liver microsomes from rats

1987 ◽  
Vol 36 (7) ◽  
pp. 1053-1057 ◽  
Author(s):  
Tohru Horie ◽  
Mitsukazu Kitada ◽  
Yoshio Tanabe ◽  
Yoshio Kanakubo
Keyword(s):  
Sex Difference ◽  
Liver Microsomes ◽  
Monooxygenase System

Characterization of the cytochrome P-450 monooxygenase system of hamster liver microsomes

1987 ◽  
Vol 36 (21) ◽  
pp. 3613-3619 ◽  
Author(s):  
C.Murray Ardies ◽  
Jerome M. Lasker ◽  
Charles S. Lieber
Keyword(s):  
Liver Microsomes ◽  
Monooxygenase System ◽  
Cytochrome P 450

scholarly journals ACTIVITY OF THE MICROSOMAL OXIDATION SYSTEM IN RAT LIVER UNDER THE INFLUENCE OF SODIUM FLUORIDE

2021 ◽  
pp. 121-124
Author(s):  
I. Yu. Bagmut ◽  
I. L. Kolisnyk
Keyword(s):  
Rat Liver ◽  
Sodium Fluoride ◽  
Liver Microsomes ◽  
Rat Hepatocytes ◽  
The Body ◽  
Endogenous Respiration ◽  
Monooxygenase System ◽  
Rat Liver Microsomes ◽  
Microsomal Oxidation ◽  
Fluoride Intoxication

Summary. The pathogenesis of fluoride intoxication at the molecular, cellular and functional levels has not been sufficiently studied. There are very few modern data on these issues, so they are contradictory, since the effects of this trace element are multifaceted and cannot be characterized unambiguously. The aim of the study – to learn the state of the monooxygenase system of rat hepatocytes under conditions of the formation of fluoride intoxication. Materials and Methods. In the experiment, we used 30 sexually mature rats (N=30) of the Wistar population weighing 200–210 g for 1.5 months. Sodium fluoride solution was administered orally at doses of 1/10 DL50, which was 20 mg/kg of animal body weight. Results. The results of experiments on the study of oxygen consumption by rat liver microsomes under fluoride intoxication indicated that the rate of endogenous respiration of microsomes, the rate of NADPH oxidation, the rate of NADH oxidation in the presence of EDTA, and the rate of lipid peroxidation increase under the influence of fluorides. Sodium fluoride stimulated an increase in all parameters of microsomal oxidation, except for cytochrome b5. It should be assumed that in this case there is an increase in the generation of reactive oxygen species, free radicals, which stimulate the development of free radical processes in the body and are, most likely, the leading link in oxidative stress. Conclusions. These changes indicate a violation of the bioenergetics of hepatocytes associated with the mitochondrial apparatus and the development of hypoxic processes, which lead to a decrease in the activity of redox reactions occurring at the level of intracellular membranes and organelles.

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