Molecular cloning and sequence analysis of the sisomicin biosynthetic gene cluster from Micromonospora inyoensis

2008 ◽  
Vol 31 (3) ◽  
pp. 449-455 ◽  
Author(s):  
Wun-Rong Hong ◽  
Mei Ge ◽  
Zhi-Hong Zeng ◽  
Li Zhu ◽  
Min-Yu Luo ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Molecular Cloning ◽  
Gene Cluster ◽  
Biosynthetic Gene Cluster ◽  
Biosynthetic Gene

scholarly journals Molecular cloning and sequence analysis of the complestatin biosynthetic gene cluster

2001 ◽  
Vol 98 (15) ◽  
pp. 8548-8553 ◽  
Author(s):  
H.-T. Chiu ◽  
B. K. Hubbard ◽  
A. N. Shah ◽  
J. Eide ◽  
R. A. Fredenburg ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Molecular Cloning ◽  
Gene Cluster ◽  
Biosynthetic Gene Cluster ◽  
Biosynthetic Gene

scholarly journals Molecular cloning and sequence analysis of the sisomicin biosynthetic gene cluster from Micromonospora inyoensis

2009 ◽  
Vol 31 (7) ◽  
pp. 1115-1115
Author(s):  
Wen-Rong Hong ◽  
Mei Ge ◽  
Zhi-Hong Zeng ◽  
Li Zhu ◽  
Min-Yu Luo ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Molecular Cloning ◽  
Gene Cluster ◽  
Biosynthetic Gene Cluster ◽  
Biosynthetic Gene

Molecular cloning and characterization of an ML-236B (compactin) biosynthetic gene cluster in Penicillium citrinum

2002 ◽  
Vol 267 (5) ◽  
pp. 636-646 ◽  
Author(s):  
Y. Abe ◽  
T. Suzuki ◽  
C. Ono ◽  
K. Iwamoto ◽  
M. Hosobuchi ◽  
...  
Keyword(s):  
Molecular Cloning ◽  
Gene Cluster ◽  
Biosynthetic Gene Cluster ◽  
Penicillium Citrinum ◽  
Biosynthetic Gene

scholarly journals The granaticin biosynthetic gene cluster of Streptomyces violaceoruber Tü22: sequence analysis and expression in a heterologous host

1998 ◽  
Vol 5 (11) ◽  
pp. 647-659 ◽  
Author(s):  
Koji Ichinose ◽  
David J. Bedford ◽  
Diethild Tornus ◽  
Andreas Bechthold ◽  
Maureen J. Bibb ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Gene Cluster ◽  
Biosynthetic Gene Cluster ◽  
Biosynthetic Gene ◽  
Heterologous Host ◽  
Streptomyces Violaceoruber

scholarly journals Molecular Cloning, Sequence Analysis, and Heterologous Expression of the Phosphinothricin Tripeptide Biosynthetic Gene Cluster from Streptomyces viridochromogenes DSM 40736

2005 ◽  
Vol 49 (1) ◽  
pp. 230-240 ◽  
Author(s):  
Joshua A. V. Blodgett ◽  
Jun Kai Zhang ◽  
William W. Metcalf
Keyword(s):  
Sequence Analysis ◽  
Heterologous Expression ◽  
Gene Cluster ◽  
Genomic Dna ◽  
Biosynthetic Gene Cluster ◽  
Fosmid Library ◽  
Open Reading Frames ◽  
Biosynthetic Gene ◽  
Heterologous Host ◽  
Reading Frames

ABSTRACT A fosmid library from genomic DNA of Streptomyces viridochromogenes DSM 40736 was constructed and screened for the presence of genes known to be involved in the biosynthesis of phosphinothricin tripeptide (PTT). Eight positives were identified, one of which was able to confer PTT biosynthetic capability upon Streptomyces lividans after integration of the fosmid into the chromosome of this heterologous host. Sequence analysis of the 40,241-bp fosmid insert revealed 29 complete open reading frames (ORFs). Deletion analysis demonstrated that a minimum set of 24 ORFs were required for PTT production in the heterologous host. Sequence analysis revealed that most of these PTT genes have been previously identified in either S. viridochromogenes or S. hygroscopicus (or both), although only 11 out of 24 of these ORFs have experimentally defined functions. Three previously unknown genes within the cluster were identified and are likely to have roles in the stepwise production of phosphonoformate from phosphonoacetaldehyde. This is the first report detailing the entire PTT gene cluster from any producing streptomycete.

scholarly journals Cloning and Characterization of the Pyrrolomycin Biosynthetic Gene Clusters from Actinosporangium vitaminophilum ATCC 31673 and Streptomyces sp. Strain UC 11065

2006 ◽  
Vol 51 (3) ◽  
pp. 946-957 ◽  
Author(s):  
Xiujun Zhang ◽  
Ronald J. Parry
Keyword(s):  
Sequence Analysis ◽  
Gene Cluster ◽  
Genetic Locus ◽  
Gene Clusters ◽  
Biosynthetic Gene Cluster ◽  
Open Reading Frames ◽  
Biosynthetic Gene ◽  
Genes Encoding ◽  
Cloned Gene

ABSTRACT The pyrrolomycins are a family of polyketide antibiotics, some of which contain a nitro group. To gain insight into the nitration mechanism associated with the formation of these antibiotics, the pyrrolomycin biosynthetic gene cluster from Actinosporangium vitaminophilum was cloned. Sequencing of ca. 56 kb of A. vitaminophilum DNA revealed 35 open reading frames (ORFs). Sequence analysis revealed a clear relationship between some of these ORFs and the biosynthetic gene cluster for pyoluteorin, a structurally related antibiotic. Since a gene transfer system could not be devised for A. vitaminophilum, additional proof for the identity of the cloned gene cluster was sought by cloning the pyrrolomycin gene cluster from Streptomyces sp. strain UC 11065, a transformable pyrrolomycin producer. Sequencing of ca. 26 kb of UC 11065 DNA revealed the presence of 17 ORFs, 15 of which exhibit strong similarity to ORFs in the A. vitaminophilum cluster as well as a nearly identical organization. Single-crossover disruption of two genes in the UC 11065 cluster abolished pyrrolomycin production in both cases. These results confirm that the genetic locus cloned from UC 11065 is essential for pyrrolomycin production, and they also confirm that the highly similar locus in A. vitaminophilum encodes pyrrolomycin biosynthetic genes. Sequence analysis revealed that both clusters contain genes encoding the two components of an assimilatory nitrate reductase. This finding suggests that nitrite is required for the formation of the nitrated pyrrolomycins. However, sequence analysis did not provide additional insights into the nitration process, suggesting the operation of a novel nitration mechanism.

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