Characterization of the optimized C2 domain of protein G: finding its additional chicken IgY-binding ability

2013 ◽  
Vol 35 (9) ◽  
pp. 1441-1447 ◽  
Author(s):  
Yongsheng Cao ◽  
Di Li ◽  
Chong Cao ◽  
Mingchun Gao ◽  
Runxiang Zhang ◽  
...  
Keyword(s):  
Protein G ◽  
C2 Domain ◽  
Binding Ability

scholarly journals Characterization of Multiple C2 Domain and Transmembrane Region Proteins in Arabidopsis

2017 ◽  
Vol 176 (3) ◽  
pp. 2119-2132 ◽  
Author(s):  
Lu Liu ◽  
Chunying Li ◽  
Zhe Liang ◽  
Hao Yu
Keyword(s):  
C2 Domain ◽  
Transmembrane Region

Characterization of the Membrane Binding Mode of the C2 Domain of PKCε†

Biochemistry ◽  
2003 ◽  
Vol 42 (40) ◽  
pp. 11661-11668 ◽  
Author(s):  
Senena Corbalán-Garcia ◽  
Susana Sánchez-Carrillo ◽  
Josefa García-García ◽  
Juan C. Gómez-Fernández
Keyword(s):  
Membrane Binding ◽  
Binding Mode ◽  
C2 Domain

scholarly journals Cloning and characterization of two mouse heat shock factors with distinct inducible and constitutive DNA-binding ability.

1991 ◽  
Vol 5 (10) ◽  
pp. 1902-1911 ◽  
Author(s):  
K D Sarge ◽  
V Zimarino ◽  
K Holm ◽  
C Wu ◽  
R I Morimoto
Keyword(s):  
Heat Shock ◽  
Dna Binding ◽  
Heat Shock Factors ◽  
Binding Ability

scholarly journals Correlation between composition and activity of gold nanoparticle conjugates with streptococcal protein G

2020 ◽  
Vol 10 (2) ◽  
pp. 4988-4992
Keyword(s):  
Dissociation Constant ◽  
Equilibrium Constants ◽  
Gold Surface ◽  
Binding Activity ◽  
Protein G ◽  
Binding Ability ◽  
Adsorbed Protein ◽  
Streptococcal Protein G ◽  
The Stability ◽  
Nanoparticle Complex

The composition of the conjugates of gold nanoparticles with streptococcal protein G was studied using fluorescence spectroscopy. The method for determining the composition is based on measuring the intrinsic fluorescence of tryptophan as part of the protein. The equilibrium constants of protein binding by the gold surface were determined using the Sketchard method. An increase in the dissociation constant of the protein–nanoparticle complex for increasing the amount of bound protein was demonstrated, and a relationship was established between the stability of the conjugates, their antigen-binding activity, and the dissociation constant. The effectiveness of the conjugates of different compositions in immunochromatographic assay of specific antibodies against the lipopolysaccharide antigen of Brucella abortus was compared. The binding ability of the conjugates increased along with the amount of protein G to ~200 molecules per nanoparticle. A further increase in the amount of adsorbed protein led to a deterioration in the functional activity of the conjugates.

Molecular characterization of a pepper C2 domain-containing SRC2 protein implicated in resistance against host and non-host pathogens and abiotic stresses

Planta ◽  
2008 ◽  
Vol 227 (5) ◽  
pp. 1169-1179 ◽  
Author(s):  
Young-Cheol Kim ◽  
Soo-Yong Kim ◽  
Doil Choi ◽  
Choong-Min Ryu ◽  
Jeong Mee Park
Keyword(s):  
Molecular Characterization ◽  
Abiotic Stresses ◽  
C2 Domain

Cloning and Characterization of BAP3 (BAI-Associated Protein 3), a C2 Domain-Containing Protein That Interacts with BAI1

1998 ◽  
Vol 251 (1) ◽  
pp. 158-165 ◽  
Author(s):  
Takayuki Shiratsuchi ◽  
Katsutoshi Oda ◽  
Hiroyuki Nishimori ◽  
Mikio Suzuki ◽  
Eiichi Takahashi ◽  
...  
Keyword(s):  
C2 Domain
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