Biochemical characterization of xylanase GH11 isolated from Aspergillus niger BCC14405 (XylB) and its application in xylooligosaccharide production

2021 ◽  
Author(s):  
Katesuda Aiewviriyasakul ◽  
Benjarat Bunterngsook ◽  
Hataikarn Lekakarn ◽  
Wipawee Sritusnee ◽  
Pattanop Kanokratana ◽  
...  
Keyword(s):  
Aspergillus Niger ◽  
Biochemical Characterization

scholarly journals Biochemical characterization of a glycoside hydrolase family 43 β-D-galactofuranosidase from the fungus Aspergillus niger

2021 ◽  
Author(s):  
Gregory S Bulmer ◽  
Fang Wei Yuen ◽  
Naimah Begum ◽  
Bethan S Jones ◽  
Sabine S Flitsch ◽  
...  
Keyword(s):  
Aspergillus Niger ◽  
Glycoside Hydrolase ◽  
Biochemical Characterization ◽  
Maximum Activity ◽  
Glycoside Hydrolase Family ◽  
Enzyme Specificity ◽  
Fungal Enzymes ◽  
Glycoside Hydrolase Family 43 ◽  
Hydrolase Family

β-D-Galactofuranose (Galf) and its polysaccharides are found in bacteria, fungi and protozoa but do not occur in mammalian tissues, and thus represent a specific target for anti-pathogenic drugs. Understanding the enzymatic degradation of these polysaccharides is therefore of great interest, but the identity of fungal enzymes with exclusively galactofuranosidase activity has so far remained elusive. Here we describe the identification and characterization of a galactofuranosidase from the industrially important fungus Aspergillus niger. Phylogenetic analysis of glycoside hydrolase family 43 subfamily 34 (GH43_34) members revealed the occurrence of three distinct clusters and, by comparison with specificities of characterized bacterial members, suggested a basis for prediction of enzyme specificity. Using this rationale, in tandem with molecular docking, we identified a putative β-D-galactofuranosidase from A. niger which was recombinantly expressed in Escherichia coli. The Galf-specific hydrolase, encoded by xynD demonstrates maximum activity at pH 5, 25 °C towards 4-Nitrophenyl-β-galactofuranoside (pNP-βGalf), with a Km of 17.9 ± 1.9 mM and Vmax of 70.6 ± 5.3 μmol min-1. The characterization of this first fungal GH43 galactofuranosidase offers further molecular insight into the degradation of Galf-containing structures and may inform clinical treatments against fungal pathogens.

Purification and biochemical characterization of a novel thermostable lichenase from Aspergillus niger US368

2013 ◽  
Vol 98 (1) ◽  
pp. 967-975 ◽  
Author(s):  
Fatma Elgharbi ◽  
Aïda Hmida-Sayari ◽  
Mouna Sahnoun ◽  
Radhouane Kammoun ◽  
Lobna Jlaeil ◽  
...  
Keyword(s):  
Aspergillus Niger ◽  
Biochemical Characterization

scholarly journals Biochemical characterization of two new Aspergillus niger aspartic proteases

3 Biotech ◽  
2020 ◽  
Vol 10 (7) ◽  
Author(s):  
Peng Song ◽  
Lei Cheng ◽  
Kangming Tian ◽  
Meng Zhang ◽  
Nokuthula Peace Mchunu ◽  
...  
Keyword(s):  
Aspergillus Niger ◽  
Biochemical Characterization ◽  
Aspartic Proteases

scholarly journals Biochemical characterization of three Aspergillus niger β-galactosidases

2017 ◽  
Vol 27 ◽  
pp. 37-43 ◽  
Author(s):  
Dandan Niu ◽  
Xiaojing Tian ◽  
Nokuthula Peace Mchunu ◽  
Chao Jia ◽  
Suren Singh ◽  
...  
Keyword(s):  
Aspergillus Niger ◽  
Biochemical Characterization
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