Effect of different oxygen availability on the nitrate reductase activity in Cucumis sativus roots

2008 ◽  
Vol 52 (4) ◽  
pp. 674-680 ◽  
Author(s):  
M. Reda ◽  
G. Klobus
2014 ◽  
Vol 49 (3) ◽  
pp. 259-267 ◽  
Author(s):  
Józef Buczek ◽  
Ewa Kowalińska ◽  
Krystyna Kuczera

Investigations were conducted on the influence of tungsten (Na<sub>2</sub>WO<sub>4</sub>) and meta-vanadate (NaVO<sub>3</sub>) on uptake and reduction of nitrates by cucumber seedlings. Tungsten and vanadium almost completely inhibited nitrate reductase activity (NR) after treating the plants for 24 hours with nitrates in the presence of 10<sup>-4</sup> M WO<sub>4</sub><sup>-2</sup> and 10<sup>-3</sup> M VO<sub>3</sub><sup>-</sup>. Uptake of NO<sub>3</sub><sup>-</sup> in the presence of WO<sub>4</sub><sup>-2</sup> in this same period of time was lowered by about 50 %, while in the presence of VO<sub>3</sub><sup>-</sup> by seventy percent. The ratio of NO<sub>3</sub><sup>-</sup> absorbed to that reducted in control plants was around 3, whereas in the presence of WO<sub>4</sub><sup>-2</sup> and VO<sub>3</sub><sup>-</sup> 9 and 8 respectively. The effect of NR inhibiting activity by WO<sub>4</sub><sup>-2</sup> and VO<sub>3</sub><sup>-</sup> was significant but somewhat weaker if both inhibitors were applied to the plants 24 hours after formation of the NR system, whereas NO<sub>3</sub><sup>-</sup> uptake was subject to a slight lowering. The above data suggest that WO<sub>4</sub><sup>-2</sup> and VO<sub>3</sub><sup>-</sup> after a 24 hour application of these inhibitors to plants, primary inhibit biosynthesis and activity of NR and to a lesser extent effect NO<sub>3</sub><sup>-</sup> absorption. On the basis of results reached the Butz and Jackson (1977) hypothesis concerning the model of NO<sub>3</sub><sup>-</sup> uptake and reduction by plant tissues is discussed.


2014 ◽  
Vol 57 (3) ◽  
pp. 349-359 ◽  
Author(s):  
Marek Burzyński

Treatment of 4-day-old cucumber (<em>Cucumis sativus</em> L.) seedlings with PbCl<sub>2</sub> or CdCl<sub>2</sub> caused a significant increase in the accumulation of heavy metals by the plants, especially in the roots. The accumulated Pb initially enhanced the uptake of phosphorous after the plants had been transferred to a nutrient medium (6, 24 hrs), but after only 48 Ins the uptake had dropped to below control level. The plants treated with Cd exhibited a constant decreased phosphorous uptake level. The accumulated lead and cadmium also inhibited nitrate uptake and the activity of nitrate reductase. It is suggested that the reason for the decreased nitrate reductase activity lay rather in the lower nitrate uptake than in a direct effect of the heavy metals on the enzyme.


Plant Science ◽  
2001 ◽  
Vol 161 (2) ◽  
pp. 231-237 ◽  
Author(s):  
Purificación de la Haba ◽  
Eloisa Agüera ◽  
Lourdes Benı́tez ◽  
José M. Maldonado

Crop Science ◽  
1966 ◽  
Vol 6 (2) ◽  
pp. 169-173 ◽  
Author(s):  
L. E. Schrader ◽  
D. M. Peterson ◽  
E. R. Leng ◽  
R. H. Hageman

Crop Science ◽  
1982 ◽  
Vol 22 (1) ◽  
pp. 85-88 ◽  
Author(s):  
E. L. Deckard ◽  
N. D. Williams ◽  
J. J. Hammond ◽  
L. R. Joppa

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