Efficient virus-induced gene silencing in Brassica rapa using a turnip yellow mosaic virus vector

2018 ◽  
Vol 62 (4) ◽  
pp. 826-834 ◽  
Author(s):  
J. Yu ◽  
X.-D. Yang ◽  
Q. Wang ◽  
L.-W. Gao ◽  
Y. Yang ◽  
...  
Keyword(s):  
Gene Silencing ◽  
Mosaic Virus ◽  
Brassica Rapa ◽  
Virus Vector ◽  
Yellow Mosaic Virus ◽  
Turnip Yellow Mosaic Virus ◽  
Virus Induced Gene Silencing

scholarly journals A Foxtail mosaic virus Vector for Virus-Induced Gene Silencing in Maize

2016 ◽  
pp. pp.00172.2016 ◽  
Author(s):  
Yu Mei ◽  
Chunquan Zhang ◽  
Bliss M. Kernodle ◽  
John H. Hill ◽  
Steven A. Whitham
Keyword(s):  
Gene Silencing ◽  
Mosaic Virus ◽  
Virus Vector ◽  
Virus Induced Gene Silencing

scholarly journals A High Throughput Barley Stripe Mosaic Virus Vector for Virus Induced Gene Silencing in Monocots and Dicots

PLoS ONE ◽  
2011 ◽  
Vol 6 (10) ◽  
pp. e26468 ◽  
Author(s):  
Cheng Yuan ◽  
Cui Li ◽  
Lijie Yan ◽  
Andrew O. Jackson ◽  
Zhiyong Liu ◽  
...  
Keyword(s):  
Gene Silencing ◽  
Mosaic Virus ◽  
High Throughput ◽  
Virus Vector ◽  
Barley Stripe Mosaic Virus ◽  
Virus Induced Gene Silencing

scholarly journals Discriminating Mutations of HC-Pro of Zucchini yellow mosaic virus with Differential Effects on Small RNA Pathways Involved in Viral Pathogenicity and Symptom Development

2010 ◽  
Vol 23 (1) ◽  
pp. 17-28 ◽  
Author(s):  
Hui-Wen Wu ◽  
Shih-Shun Lin ◽  
Kuan-Chun Chen ◽  
Shyi-Dong Yeh ◽  
Nam-Hai Chua
Keyword(s):  
Amino Acids ◽  
Gene Silencing ◽  
Mosaic Virus ◽  
Symptom Severity ◽  
Zucchini Yellow Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
Transcriptional Gene Silencing ◽  
Molecular Evidence ◽  
Virus Induced Gene Silencing ◽  
Conserved Amino Acids

Helper component-proteinase (HC-Pro), the gene-silencing suppressor of Potyvirus spp., interferes with microRNA (miRNA) and short-interfering RNA (siRNA) pathways. Our previous studies showed that three mutations of highly conserved amino acids of HC-Pro, R180I (mutation A), F205L (B), and E396N (C), of Zucchini yellow mosaic virus (ZYMV) affect symptom severity and viral pathogenicity. The mutant ZYMV GAC (ZGAC) with double mutations, R180I/E396N, induces transient leaf mottling in host plants followed by recovery. This mutant confers complete cross protection against subsequent infection by the parental ZYMV (ZG) strain. Here, we sought to obtain molecular evidence on the roles of the three highly conserved amino acids of HC-Pro in miRNA and siRNA pathways using transgenic Arabidopsis plants expressing comparable levels of wild-type and mutant HC-Pro proteins. We demonstrated that amino acid residues 180, 205, and 396 of HC-Pro are critical for suppression of miRNA, trans-acting siRNA (ta-siRNA), and virus-induced gene silencing (VIGS) pathways but not for sense-post transcriptional gene silencing (s-PTGS). Because the HC-Pro double mutant (R180I/E396N) does not interfere with miRNA and ta-siRNA pathways, the ZGAC mutant virus elicits only attenuated symptoms. Furthermore, the recovery seen on ZGAC-infected plants likely results from the weak VIGS suppression by the HC-Pro double AC mutant. Thus, through manipulating these three conserved amino acids on HC-Pro, symptom severity of diseases caused by Potyvirus spp. can be modulated to generate useful cross protectants for field application. Although some of our mutated HC-Pro proteins do not interfere with miRNA and ta-siRNA pathways, they still retain the ability to suppress s-PTGS.

scholarly journals A cucumber green mottle mosaic virus vector for virus-induced gene silencing in cucurbit plants

Plant Methods ◽  
2020 ◽  
Vol 16 (1) ◽  
Author(s):  
Mei Liu ◽  
Zhiling Liang ◽  
Miguel A. Aranda ◽  
Ni Hong ◽  
Liming Liu ◽  
...  
Keyword(s):  
Gene Silencing ◽  
Mosaic Virus ◽  
Virus Vector ◽  
Virus Induced Gene Silencing

scholarly journals Mutational bias of Turnip Yellow Mosaic Virus in the context of host anti-viral gene silencing

Virology ◽  
2015 ◽  
Vol 486 ◽  
pp. 2-6 ◽  
Author(s):  
Jinmin Ma ◽  
Denise Pallett ◽  
Hui Jiang ◽  
Yong Hou ◽  
Hui Wang
Keyword(s):  
Gene Silencing ◽  
Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
Mutational Bias ◽  
Viral Gene ◽  
Turnip Yellow Mosaic Virus
Sign in / Sign up

Export Citation Format

Share Document