Stem cell marker olfactomedin 4: critical appraisal of its characteristics and role in tumorigenesis

2010 ◽  
Vol 29 (4) ◽  
pp. 761-775 ◽  
Author(s):  
Phulwinder K. Grover ◽  
Jennifer E. Hardingham ◽  
Adrian G. Cummins
Keyword(s):  
Stem Cell ◽  
Critical Appraisal ◽  
Stem Cell Marker ◽  
Cell Marker

The Corneal Epithelial Stem Cell Marker - Novel Combination of High Expression of p63 and a Large N/C Ratio

2008 ◽  
Vol 1 (3) ◽  
pp. 95-105
Author(s):  
Arpitha P. ◽  
Prajna N. V. ◽  
Srinivasan M. ◽  
Muthukkaruppan V. R.
Keyword(s):  
Stem Cell ◽  
High Expression ◽  
Stem Cell Marker ◽  
Cell Marker ◽  
Corneal Epithelial ◽  
Epithelial Stem Cell ◽  
Large N

Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells

PROTEOMICS ◽  
2021 ◽  
pp. 2000098
Author(s):  
Annalisa L.E. Carli ◽  
Shoukat Afshar‐Sterle ◽  
Alin Rai ◽  
Haoyun Fang ◽  
Ryan O'Keefe ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Stem Cell ◽  
Cancer Stem Cell ◽  
Cancer Cells ◽  
Extracellular Vesicles ◽  
Stem Cell Marker ◽  
Cancer Stem Cell Marker ◽  
Cell Marker ◽  
Gastric Cancer Cells ◽  
Migratory Phenotype

scholarly journals Potential roles of stem cell marker genes in axon regeneration

2021 ◽  
Vol 53 (1) ◽  
pp. 1-7
Author(s):  
Jinyoung Lee ◽  
Yongcheol Cho
Keyword(s):  
Stem Cell ◽  
Expression Profiling ◽  
Axon Regeneration ◽  
Stem Cell Marker ◽  
Marker Genes ◽  
Cell Marker ◽  
Regenerative Capacity ◽  
Regeneration Mechanism ◽  
Central Nervous Systems

AbstractAxon regeneration is orchestrated by many genes that are differentially expressed in response to injury. Through a comparative analysis of gene expression profiling, injury-responsive genes that are potential targets for understanding the mechanisms underlying regeneration have been revealed. As the efficiency of axon regeneration in both the peripheral and central nervous systems can be manipulated, we suggest that identifying regeneration-associated genes is a promising approach for developing therapeutic applications in vivo. Here, we review the possible roles of stem cell marker- or stemness-related genes in axon regeneration to gain a better understanding of the regeneration mechanism and to identify targets that can enhance regenerative capacity.

scholarly journals Differential SOD2 and GSTZ1 profiles contribute to contrasting dental pulp stem cell susceptibilities to oxidative damage and premature senescence

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Nadia Y. A. Alaidaroos ◽  
Amr Alraies ◽  
Rachel J. Waddington ◽  
Alastair J. Sloan ◽  
Ryan Moseley
Keyword(s):  
Stem Cell ◽  
Regenerative Medicine ◽  
Oxidative Damage ◽  
Dental Pulp ◽  
Stem Cell Marker ◽  
Significant Heterogeneity ◽  
Premature Senescence ◽  
Cell Marker ◽  
Marker Expression ◽  
The Impact

Abstract Background Dental pulp stem cells (DPSCs) are increasingly being advocated as viable cell sources for regenerative medicine-based therapies. However, significant heterogeneity in DPSC expansion and multi-potency capabilities are well-established, attributed to contrasting telomere profiles and susceptibilities to replicative senescence. As DPSCs possess negligible human telomerase (hTERT) expression, we examined whether intrinsic differences in the susceptibilities of DPSC sub-populations to oxidative stress-induced biomolecular damage and premature senescence further contributed to this heterogeneity, via differential enzymic antioxidant capabilities between DPSCs. Methods DPSCs were isolated from human third molars by differential fibronectin adhesion, and positive mesenchymal (CD73/CD90/CD105) and negative hematopoietic (CD45) stem cell marker expression confirmed. Isolated sub-populations were expanded in H2O2 (0–200 μM) and established as high or low proliferative DPSCs, based on population doublings (PDs) and senescence (telomere lengths, SA-β-galactosidase, p53/p16INK4a/p21waf1/hTERT) marker detection. The impact of DPSC expansion on mesenchymal, embryonic, and neural crest marker expression was assessed, as were the susceptibilities of high and low proliferative DPSCs to oxidative DNA and protein damage by immunocytochemistry. Expression profiles for superoxide dismutases (SODs), catalase, and glutathione-related antioxidants were further compared between DPSC sub-populations by qRT-PCR, Western blotting and activity assays. Results High proliferative DPSCs underwent > 80PDs in culture and resisted H2O2−induced senescence (50–76PDs). In contrast, low proliferative sub-populations exhibited accelerated senescence (4–32PDs), even in untreated controls (11-34PDs). While telomere lengths were largely unaffected, certain stem cell marker expression declined with H2O2 treatment and expansion. Elevated senescence susceptibilities in low proliferative DPSC (2–10PDs) were accompanied by increased oxidative damage, absent in high proliferative DPSCs until 45–60PDs. Increased SOD2/glutathione S-transferase ζ1 (GSTZ1) expression and SOD activities were identified in high proliferative DPSCs (10–25PDs), which declined during expansion. Low proliferative DPSCs (2–10PDs) exhibited inferior SOD, catalase and glutathione-related antioxidant expression/activities. Conclusions Significant variations exist in the susceptibilities of DPSC sub-populations to oxidative damage and premature senescence, contributed to by differential SOD2 and GSTZ1 profiles which maintain senescence-resistance/stemness properties in high proliferative DPSCs. Identification of superior antioxidant properties in high proliferative DPSCs enhances our understanding of DPSC biology and senescence, which may be exploited for selective sub-population screening/isolation from dental pulp tissues for regenerative medicine-based applications.

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