Establishment of serum-free adapted Chinese hamster ovary cells with double knockout of GDP-mannose-4,6-dehydratase and GDP-fucose transporter

2022 ◽  
Author(s):  
Ryo Misaki ◽  
Masashi Iwasaki ◽  
Hiroki Takechi ◽  
Noriko Yamano-Adachi ◽  
Takao Ohashi ◽  
...  
Keyword(s):  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Double Knockout ◽  
Ovary Cells ◽  
Serum Free

scholarly journals Polyamine starvation causes accumulation of cadaverine and its derivatives in a polyamine-dependent strain of Chinese-hamster ovary cells

1983 ◽  
Vol 210 (3) ◽  
pp. 945-948 ◽  
Author(s):  
E Hölttä ◽  
P Pohjanpelto
Keyword(s):  
Cell Proliferation ◽  
Ornithine Decarboxylase ◽  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Specific Inhibitor ◽  
Ovary Cells ◽  
Serum Free ◽  
Dependent Strain

Starvation of the polyamine-dependent Chinese-hamster ovary cells for ornithine or ornithine-derived polyamines in serum-free culture resulted in the formation of cadaverine and its aminopropyl derivatives, N-(3-aminopropyl)cadaverine and NN'-bis(3-aminopropyl)cadaverine. The synthesis of these unusual amines was inhibited by treatment of the cells with DL-2-difluoromethylornithine, a specific inhibitor of ornithine decarboxylase (EC 4.1.1.17). In the absence of ornithine (the normal substrate), ornithine decarboxylase thus appeared to catalyse the decarboxylation of lysine to cadaverine. Cell proliferation was markedly inhibited by ornithine deprivation of the cells, and further depressed by exposure of the cultures to difluoromethylornithine.

The impact of cell adaptation to serum-free conditions on the glycosylation profile of a monoclonal antibody produced by Chinese hamster ovary cells

2013 ◽  
Vol 30 (5) ◽  
pp. 563-572 ◽  
Author(s):  
Ana Rita Costa ◽  
Joanne Withers ◽  
Maria Elisa Rodrigues ◽  
Niaobh McLoughlin ◽  
Mariana Henriques ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Ovary Cells ◽  
Serum Free ◽  
Glycosylation Profile ◽  
Cell Adaptation ◽  
The Impact ◽  
Serum Free Conditions

Cell cycle analysis of Chinese hamster ovary cells stimulated by phosphatidic acid in serum-free culture

2004 ◽  
Vol 98 (6) ◽  
pp. 487-489 ◽  
Author(s):  
Hideki Yamaji ◽  
Kentaro Sakai ◽  
Tsukasa Joho ◽  
Eiji Izumoto ◽  
Hideki Fukuda
Keyword(s):  
Cell Cycle ◽  
Phosphatidic Acid ◽  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Cell Cycle Analysis ◽  
Ovary Cells ◽  
Serum Free

scholarly journals Fetal calf serum-free culture of Chinese hamster ovary cells employing fish serum

2007 ◽  
Vol 75 (5) ◽  
pp. 983-987 ◽  
Author(s):  
M. Fujiwara ◽  
R. Tsukada ◽  
Y. Tsujinaga ◽  
M. Takagi
Keyword(s):  
Chinese Hamster Ovary ◽  
Fetal Calf Serum ◽  
Chinese Hamster Ovary Cells ◽  
Calf Serum ◽  
Chinese Hamster ◽  
Ovary Cells ◽  
Serum Free ◽  
Fish Serum
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