Establishment of serum-free adapted Chinese hamster ovary cells with double knockout of GDP-mannose-4,6-dehydratase and GDP-fucose transporter

2022 ◽  
Author(s):  
Ryo Misaki ◽  
Masashi Iwasaki ◽  
Hiroki Takechi ◽  
Noriko Yamano-Adachi ◽  
Takao Ohashi ◽  
...  
Keyword(s):  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Double Knockout ◽  
Ovary Cells ◽  
Serum Free

scholarly journals Polyamine starvation causes accumulation of cadaverine and its derivatives in a polyamine-dependent strain of Chinese-hamster ovary cells

1983 ◽  
Vol 210 (3) ◽  
pp. 945-948 ◽  
Author(s):  
E Hölttä ◽  
P Pohjanpelto
Keyword(s):  
Cell Proliferation ◽  
Ornithine Decarboxylase ◽  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Specific Inhibitor ◽  
Ovary Cells ◽  
Serum Free ◽  
Dependent Strain

Starvation of the polyamine-dependent Chinese-hamster ovary cells for ornithine or ornithine-derived polyamines in serum-free culture resulted in the formation of cadaverine and its aminopropyl derivatives, N-(3-aminopropyl)cadaverine and NN'-bis(3-aminopropyl)cadaverine. The synthesis of these unusual amines was inhibited by treatment of the cells with DL-2-difluoromethylornithine, a specific inhibitor of ornithine decarboxylase (EC 4.1.1.17). In the absence of ornithine (the normal substrate), ornithine decarboxylase thus appeared to catalyse the decarboxylation of lysine to cadaverine. Cell proliferation was markedly inhibited by ornithine deprivation of the cells, and further depressed by exposure of the cultures to difluoromethylornithine.

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