In situ conservation of the wild relative of the common bean (Phaseolus vulgaris var. aborigineus) at the south of its neotropical distribution: environmental characterization of a population in central Argentina

2014 ◽  
Vol 62 (1) ◽  
pp. 115-129 ◽  
Author(s):  
H. Sirolli ◽  
S. I. Drewes ◽  
P. I. Picca ◽  
F. A. Kalesnik
Keyword(s):  
Phaseolus Vulgaris ◽  
Common Bean ◽  
In Situ Conservation ◽  
Wild Relative ◽  
The South ◽  
Central Argentina ◽  
Environmental Characterization ◽  
The Common

scholarly journals Genetic characterization at the species and symbiovar level of indigenous rhizobial isolates nodulating Phaseolus vulgaris in Greece

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Evdoxia Efstathiadou ◽  
Georgia Ntatsi ◽  
Dimitrios Savvas ◽  
Anastasia P. Tampakaki
Keyword(s):  
Phaseolus Vulgaris ◽  
Common Bean ◽  
Phylogenetic Affiliation ◽  
Rhizobial Inoculation ◽  
Rhizobial Species ◽  
The Common ◽  
First Time ◽  
Of Greece ◽  
Rhizobial Isolates

AbstractPhaseolus vulgaris (L.), commonly known as bean or common bean, is considered a promiscuous legume host since it forms nodules with diverse rhizobial species and symbiovars. Most of the common bean nodulating rhizobia are mainly affiliated to the genus Rhizobium, though strains belonging to Ensifer, Pararhizobium, Mesorhizobium, Bradyrhizobium, and Burkholderia have also been reported. This is the first report on the characterization of bean-nodulating rhizobia at the species and symbiovar level in Greece. The goals of this research were to isolate and characterize rhizobia nodulating local common bean genotypes grown in five different edaphoclimatic regions of Greece with no rhizobial inoculation history. The genetic diversity of the rhizobial isolates was assessed by BOX-PCR and the phylogenetic affiliation was assessed by multilocus sequence analysis (MLSA) of housekeeping and symbiosis-related genes. A total of fifty fast-growing rhizobial strains were isolated and representative isolates with distinct BOX-PCR fingerpriniting patterns were subjected to phylogenetic analysis. The strains were closely related to R. anhuiense, R. azibense, R. hidalgonense, R. sophoriradicis, and to a putative new genospecies which is provisionally named as Rhizobium sp. I. Most strains belonged to symbiovar phaseoli carrying the α-, γ-a and γ-b alleles of nodC gene, while some of them belonged to symbiovar gallicum. To the best of our knowledge, it is the first time that strains assigned to R. sophoriradicis and harbored the γ-b allele were found in European soils. All strains were able to re-nodulate their original host, indicating that they are true microsymbionts of common bean.

scholarly journals Isolation and characterization of endophytic bacteria isolated from the leaves of the common bean (Phaseolus vulgaris)

2012 ◽  
Vol 43 (4) ◽  
pp. 1562-1575 ◽  
Author(s):  
Leonardo Emanuel de Oliveira Costa ◽  
Marisa Vieira de Queiroz ◽  
Arnaldo Chaer Borges ◽  
Celia Alencar de Moraes ◽  
Elza Fernandes de Araújo
Keyword(s):  
Phaseolus Vulgaris ◽  
Common Bean ◽  
Endophytic Bacteria ◽  
Isolation And Characterization ◽  
The Common

scholarly journals Characterization of common bean wild populations for their in situ conservation in Northwestern Argentina

Euphytica ◽  
2017 ◽  
Vol 213 (8) ◽  
Author(s):  
Mariana J. Ferreyra ◽  
M. Carmen Menéndez-Sevillano ◽  
Yanina Noe ◽  
Luis R. Ibarra ◽  
Antonio M. De Ron
Keyword(s):  
Common Bean ◽  
In Situ Conservation ◽  
Wild Populations ◽  
Northwestern Argentina

scholarly journals Biochemical and Molecular Characterization of PvNTD2, a Nucleotidase Highly Expressed in Nodules from Phaseolus vulgaris

Plants ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 171
Author(s):  
Galvez-Valdivieso ◽  
Delgado-Garcia ◽  
Diaz-Baena ◽  
Montaño ◽  
Quiles ◽  
...  
Keyword(s):  
Phaseolus Vulgaris ◽  
Common Bean ◽  
Genetic Material ◽  
Nodule Development ◽  
Leaf Ontogeny ◽  
Purine Nucleotides ◽  
Haloacid Dehalogenase ◽  
Optimum Ph ◽  
The Common

Nucleotides are molecules of great importance in plant physiology. In addition to being elementary units of the genetic material, nucleotides are involved in bio-energetic processes, play a role as cofactors, and are also components of secondary metabolites and the hormone cytokinin. The common bean (Phaseolus vulgaris) is a legume that transports the nitrogen fixed in nodules as ureides, compounds synthetized from purine nucleotides. The first step in this pathway is the removal of the 5’-phosphate group by a phosphatase. In this study, a gene that codes for a putative nucleotidase (PvNTD2) has been identified in P. vulgaris. The predicted peptide contains the conserved domains for haloacid dehalogenase-like hydrolase superfamily. The protein has been overexpressed in Escherichia coli, and the purified protein showed molybdate-resistant phosphatase activity with nucleoside monophosphates as substrates, confirming that the identified gene codes for a nucleotidase. The optimum pH for the activity was 7–7.5. The recombinant enzyme did not show special affinity for any particular nucleotide, although the behaviour with AMP was different from that with the other nucleotides. The activity was inhibited by adenosine, and a regulatory role for this nucleoside was proposed. The expression pattern of PvNTD2 shows that it is ubiquitously expressed in all the tissues analysed, with higher expression in nodules of adult plants. The expression was maintained during leaf ontogeny, and it was induced during seedling development. Unlike PvNTD1, another NTD previously described in common bean, the high expression of PvNTD2 was maintained during nodule development, and its possible role in this organ is discussed.

scholarly journals Molecular characterization of two evolutionarily distinct endornaviruses co-infecting common bean (Phaseolus vulgaris)

2013 ◽  
Vol 94 (1) ◽  
pp. 220-229 ◽  
Author(s):  
Ryo Okada ◽  
Chee Keat Yong ◽  
Rodrigo A. Valverde ◽  
Sead Sabanadzovic ◽  
Nanako Aoki ◽  
...  
Keyword(s):  
Phaseolus Vulgaris ◽  
Common Bean ◽  
Common Property ◽  
Sequence Information ◽  
Cell Fractionation ◽  
Site Specific ◽  
Disease Symptoms ◽  
The Common ◽  
A Site

Two high-molecular-mass dsRNAs of approximately 14 and 15 kbp were isolated from the common bean (Phaseolus vulgaris) cultivar Black Turtle Soup. These dsRNAs did not appear to cause obvious disease symptoms, and were transmitted through seeds at nearly 100 % efficiency. Sequence information indicates that they are the genomes of distinct endornavirus species, for which the names Phaseolus vulgaris endornavirus 1 (PvEV-1) and Phaseolus vulgaris endornavirus 2 (PvEV-2) are proposed. The PvEV-1 genome consists of 13 908 bp and potentially encodes a single polyprotein of 4496 aa, while that of PvEV-2 consists of 14 820 bp and potentially encodes a single ORF of 4851 aa. PvEV-1 is more similar to Oryza sativa endornavirus, while PvEV-2 is more similar to bell pepper endornavirus. Both viruses have a site-specific nick near the 5′ region of the coding strand, which is a common property of the endornaviruses. Their polyproteins contain domains of RNA helicase, UDP-glycosyltransferase and RNA-dependent RNA polymerase, which are conserved in other endornaviruses. However, a viral methyltransferase domain was found in the N-terminal region of PvEV-2, but was absent in PvEV-1. Results of cell-fractionation studies suggested that their subcellular localizations were different. Most endornavirus-infected bean cultivars tested were co-infected with both viruses.

Characterization of a type A response regulator in the common bean (Phaseolus vulgaris) in response to phosphate starvation

2008 ◽  
Vol 132 (3) ◽  
pp. 272-282 ◽  
Author(s):  
Yolanda Camacho ◽  
León Martínez-Castilla ◽  
Selene Fragoso ◽  
Sonia Vázquez ◽  
Eleazar Martínez-Barajas ◽  
...  
Keyword(s):  
Phaseolus Vulgaris ◽  
Common Bean ◽  
Response Regulator ◽  
Phosphate Starvation ◽  
Type A ◽  
The Common
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