Mucus composition abnormalities in sinonasal mucosa of chronic rhinosinusitis with and without nasal polyps

Inflammation ◽  
2021 ◽  
Author(s):  
Yanyi Tu ◽  
Jing Liu ◽  
Tao Li ◽  
Xiangmin Zhou ◽  
Kai Sen Tan ◽  
...  
Keyword(s):  
Chronic Rhinosinusitis ◽  
Nasal Polyps ◽  
Sinonasal Mucosa

Chronic Rhinosinusitis with Nasal Polyps: A Proteomic Analysis

2011 ◽  
Vol 120 (12) ◽  
pp. 780-786 ◽  
Author(s):  
David C. Upton ◽  
Nathan V. Welham ◽  
John S. Kuo ◽  
Jeffery W. Walker ◽  
Thomas R. Pasic
Keyword(s):  
Chronic Rhinosinusitis ◽  
Nasal Polyps ◽  
Medical Center ◽  
Academic Medical Center ◽  
Tertiary Care ◽  
Common Disease ◽  
Protein Abundance ◽  
Protein Species ◽  
Proteomic Approach ◽  
Sinonasal Mucosa

Objectives: Chronic rhinosinusitis with nasal polyps (CRSwNP) is a severe subtype of chronic rhinosinusitis that can affect patients despite medical and surgical interventions. The purpose of this study was to utilize the techniques of proteomics to investigate differences in protein abundance within the sinonasal mucosa of patients with CRSwNP compared to healthy controls. Methods: In a case-control study at a tertiary-care academic medical center, sinonasal mucosa was harvested from 3 patients with CRSwNP and 3 control patients undergoing transsphenoidal excision of pituitary tumors. Two-dimensional gel electrophoresis was used to identify proteins with elevated or reduced abundance in CRSwNP patients compared to controls. The proteins showing the greatest abundance differences were characterized by mass spectrometry. Results: More than 300 differentially abundant proteins (p ≤ 0.05) were identified. Many of these protein species were involved in the host inflammatory response. Proteins up-regulated in CRSwNP patients included eosinophil lysophospholipase by a ratio (R) of 18.13, RHO-GDP dissociation inhibitor 2 (R = 2.80), and apolipoprotein A-1 (R = 1.73). Down-regulated proteins in CRSwNP patients included catalase (R = −5.87), annexin A1 (R = −6.27), and keratin II-8 (R = −6.73). A detailed analysis of additional protein species is outlined. Conclusions: The proteomic approach allows detection of significant differences in protein abundance in CRSwNP and provides unique insight into the pathophysiology of this common disease.

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