Molecular Structure and Crystallization Dynamics of Poly(3-hydroxyl alkanoate) with Medium-Chain Length Biosynthesized from Pseudomonas putida

2018 ◽  
Vol 26 (10) ◽  
pp. 4124-4130
Author(s):  
Takahiko Nakaoki ◽  
Takahiro Miura
Keyword(s):  
Molecular Structure ◽  
Pseudomonas Putida ◽  
Chain Length ◽  
Medium Chain ◽  
Medium Chain Length ◽  
Crystallization Dynamics

Kinetics of medium-chain-length polyhydroxyalkanoate production by a novel isolate of Pseudomonas putida LS46

2012 ◽  
Vol 58 (8) ◽  
pp. 982-989 ◽  
Author(s):  
Parveen K. Sharma ◽  
Jilagamazhi Fu ◽  
Nazim Cicek ◽  
Richard Sparling ◽  
David B. Levin
Keyword(s):  
Pseudomonas Putida ◽  
Chain Length ◽  
Saturated Fatty Acids ◽  
Dry Mass ◽  
Nucleotide Sequence Analysis ◽  
Glucose Medium ◽  
Medium Chain ◽  
Medium Chain Length ◽  
Pha Production ◽  
Nitrogen Excess

Six bacteria that synthesize medium-chain-length polyhydroxyalkanoates (mcl-PHAs) were isolated from sewage sludge and hog barn wash and identified as strains of Pseudomonas and Comamonas by 16S rDNA gene sequencing. One isolate, Pseudomonas putida LS46, showed good PHA production (22% of cell dry mass) in glucose medium, and it was selected for further studies. While it is closely related to other P. putida strains (F1, KT2440, BIRD-1, GB-1, S16, and W619), P. putida LS46 was genetically distinct from these other strains on the basis of nucleotide sequence analysis of the cpn60 gene hypervariable region. PHA production was detected as early as 12 h in both nitrogen-limited and nitrogen-excess conditions. The increase in PHA production after 48 h was higher in nitrogen-limited cultures than in nitrogen-excess cultures. Pseudomonas putida LS46 produced mcl-PHAs when cultured with glucose, glycerol, or C6–C14 saturated fatty acids as carbon sources, and mcl-PHAs accounted for 56% of the cell dry mass when cells were batch cultured in medium containing 20 mmol/L octanoate. Although 3-hydroxydecanoate was the major mcl-PHA monomer (58.1–68.8 mol%) in P. putida LS46 cultured in glucose medium, 3-hydroxyoctanoate was the major monomer produced in octanoate medium (88 mol%).

scholarly journals Draft Genome Sequence of Medium-Chain-Length Polyhydroxyalkanoate-Producing Pseudomonas putida Strain LS46

2013 ◽  
Vol 1 (2) ◽  
Author(s):  
P. K. Sharma ◽  
J. Fu ◽  
X. Zhang ◽  
B. W. Fristensky ◽  
K. Davenport ◽  
...  
Keyword(s):  
Pseudomonas Putida ◽  
Chain Length ◽  
Genome Sequence ◽  
Draft Genome ◽  
Draft Genome Sequence ◽  
Medium Chain ◽  
Medium Chain Length

scholarly journals Quantitative ‘Omics Analyses of Medium Chain Length Polyhydroxyalkanaote Metabolism in Pseudomonas putida LS46 Cultured with Waste Glycerol and Waste Fatty Acids

PLoS ONE ◽  
2015 ◽  
Vol 10 (11) ◽  
pp. e0142322 ◽  
Author(s):  
Jilagamazhi Fu ◽  
Parveen Sharma ◽  
Vic Spicer ◽  
Oleg V. Krokhin ◽  
Xiangli Zhang ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Pseudomonas Putida ◽  
Chain Length ◽  
Medium Chain ◽  
Waste Glycerol ◽  
Medium Chain Length

scholarly journals Molecular Basis of Medium-Chain Length-PHA Metabolism of Pseudomonas putida

2020 ◽  
pp. 89-114
Author(s):  
Maria Tsampika Manoli ◽  
Natalia Tarazona ◽  
Aranzazu Mato ◽  
Beatriz Maestro ◽  
Jesús M. Sanz ◽  
...  
Keyword(s):  
Pseudomonas Putida ◽  
Chain Length ◽  
Molecular Basis ◽  
Medium Chain ◽  
Medium Chain Length

Production of two monomer structures containing medium-chain-length polyhydroxyalkanoates by β-oxidation-impaired mutant of Pseudomonas putida KT2442

2009 ◽  
Vol 100 (20) ◽  
pp. 4891-4894 ◽  
Author(s):  
Long Ma ◽  
Haifeng Zhang ◽  
Qian Liu ◽  
Jiong Chen ◽  
Jing Zhang ◽  
...  
Keyword(s):  
Pseudomonas Putida ◽  
Chain Length ◽  
Medium Chain ◽  
Medium Chain Length

scholarly journals Cytochrome P450 Alkane Hydroxylases of the CYP153 Family Are Common in Alkane-Degrading Eubacteria Lacking Integral Membrane Alkane Hydroxylases

2006 ◽  
Vol 72 (1) ◽  
pp. 59-65 ◽  
Author(s):  
Jan B. van Beilen ◽  
Enrico G. Funhoff ◽  
Alexander van Loon ◽  
Andrea Just ◽  
Leo Kaysser ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Pseudomonas Putida ◽  
Chain Length ◽  
Nonheme Iron ◽  
Cytochrome P450 Enzymes ◽  
Medium Chain ◽  
Ferredoxin Reductase ◽  
Medium Chain Length ◽  
Reductase Gene ◽  
Alkane Hydroxylases

ABSTRACT Several strains that grow on medium-chain-length alkanes and catalyze interesting hydroxylation and epoxidation reactions do not possess integral membrane nonheme iron alkane hydroxylases. Using PCR, we show that most of these strains possess enzymes related to CYP153A1 and CYP153A6, cytochrome P450 enzymes that were characterized as alkane hydroxylases. A vector for the polycistronic coexpression of individual CYP153 genes with a ferredoxin gene and a ferredoxin reductase gene was constructed. Seven of the 11 CYP153 genes tested allowed Pseudomonas putida GPo12 recombinants to grow well on alkanes, providing evidence that the newly cloned P450s are indeed alkane hydroxylases.

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