Chlamydia pneumoniae infected macrophages exhibit enhanced plasma membrane fluidity and show increased adherence to endothelial cells

2005 ◽  
Vol 269 (1) ◽  
pp. 69-84 ◽  
Author(s):  
Anthony A. Azenabor ◽  
Godwin Job ◽  
Olanrewaju O. Adedokun
Keyword(s):  
Endothelial Cells ◽  
Plasma Membrane ◽  
Membrane Fluidity ◽  
Chlamydia Pneumoniae ◽  
Plasma Membrane Fluidity

Serotonin transport and fluidity in plasma membrane vesicles: effect of hyperoxia

1988 ◽  
Vol 254 (6) ◽  
pp. C781-C787 ◽  
Author(s):  
N. P. Sheridan ◽  
E. R. Block
Keyword(s):  
Endothelial Cells ◽  
Plasma Membrane ◽  
Pulmonary Artery ◽  
Membrane Fluidity ◽  
Fluorescence Probe ◽  
Membrane Vesicles ◽  
Phase System ◽  
Maximal Velocity ◽  
Plasma Membrane Vesicles ◽  
Plasma Membrane Fluidity

Plasma membrane vesicles were prepared from porcine pulmonary artery endothelial cells by a dextran-polyethylene glycol two-phase system. Specific carrier-mediated transport of 5-hydroxytryptamine (5-HT) into the vesicles was examined. Transport required a Na+ gradient (out greater than in) across the membrane, and accumulated 5-HT rapidly effluxed out of the vesicles when the ionophore gramicidin was added. Transport was inhibited by the antidepressant imipramine. 5-HT transport into plasma membrane vesicles appeared saturable and exhibited Michaelis-Menten kinetics (Km 7.4 microM, maximal velocity 217 pmol.min-1.mg membrane protein-1). A 24-h exposure to 95% O2 at 1 atmosphere absolute resulted in a 21% decrease (P less than 0.05) in specific 5-HT transport by plasma membrane vesicles. Hyperoxia also caused a significant (P less than 0.01) decrease in plasma membrane fluidity, as measured with the fluorescence probe 1,6-diphenyl-1,3,5-hexatriene. These results indicate that pulmonary artery endothelial cell plasma membrane vesicles provide a good model for studying 5-HT transport activity in vitro. Hyperoxia affects plasma membrane fluidity and 5-HT transport in pulmonary artery endothelial cells, suggesting a possible cause-and-effect relationship between the two.

Effects of eicosapentaenoic acid and docosahexaenoic acid on plasma membrane fluidity of aortic endothelial cells

Lipids ◽  
1999 ◽  
Vol 34 (12) ◽  
pp. 1297-1304 ◽  
Author(s):  
Michio Hashimoto ◽  
Md. Shahdat Hossain ◽  
Hiroshi Yamasaki ◽  
Kazunaga Yazawa ◽  
Sumio Masumura
Keyword(s):  
Endothelial Cells ◽  
Plasma Membrane ◽  
Docosahexaenoic Acid ◽  
Eicosapentaenoic Acid ◽  
Membrane Fluidity ◽  
Aortic Endothelial Cells ◽  
Plasma Membrane Fluidity ◽  
Aortic Endothelial

Effect of plasma membrane fluidity on serotonin transport by endothelial cells

1987 ◽  
Vol 253 (5) ◽  
pp. C672-C678 ◽  
Author(s):  
E. R. Block ◽  
D. Edwards
Keyword(s):  
Endothelial Cells ◽  
Plasma Membrane ◽  
Pulmonary Artery ◽  
Membrane Fluidity ◽  
Vaccenic Acid ◽  
Membrane Lipid ◽  
Side Chain ◽  
Head Group ◽  
Plasma Membrane Fluidity ◽  
Serotonin Transport

To evaluate the effect of plasma membrane fluidity of lung endothelial cells on serotonin transport, porcine pulmonary artery endothelial cells were incubated for 3 h with either 0.1 mM cholesterol hemisuccinate, 0.1 mM cis-vaccenic acid, or vehicle (control), after which plasma membrane fluidity and serotonin transport were measured. Fluorescence spectroscopy was used to measure fluidity in the plasma membrane. Serotonin uptake was calculated from the disappearance of [14C]-serotonin from the culture medium. Cholesterol decreased fluidity in the subpolar head group and central and midacyl side-chain regions of the plasma membrane and decreased serotonin transport, whereas cis-vaccenic acid increased fluidity in the central and midacyl side-chain regions of the plasma membrane and also increased serotonin transport. Cis-vaccenic acid had no effect on fluidity in the subpolar head group region of the plasma membrane. These results provide evidence that the physical state of the central and midacyl chains within the pulmonary artery endothelial cell plasma membrane lipid bilayer modulates transmembrane transport of serotonin by these cells.

Hyperoxia reduces plasma membrane fluidity: a mechanism for endothelial cell dysfunction

1986 ◽  
Vol 60 (3) ◽  
pp. 826-835 ◽  
Author(s):  
E. R. Block ◽  
J. M. Patel ◽  
K. J. Angelides ◽  
N. P. Sheridan ◽  
L. C. Garg
Keyword(s):  
Endothelial Cells ◽  
Plasma Membrane ◽  
Pulmonary Artery ◽  
Atpase Activity ◽  
Membrane Fluidity ◽  
Atp Content ◽  
Aortic Endothelial Cells ◽  
Plasma Membrane Fluidity ◽  
High O2 ◽  
Aortic Endothelial

To evaluate the relative contributions of three possible mechanisms that can be advanced to explain the observation that hyperoxia decreases serotonin uptake by endothelial cells, we examined the effect of high O2 tensions on Na+-K+-ATPase activity, ATP content, and plasma membrane fluidity in cultured endothelial cells. Confluent monolayers of pulmonary artery and aortic endothelial cells were exposed to 95% O2 (hyperoxia) or 20% O2 (controls) in 5% CO2 at 1 ATA for 4–42 h. Exposure to high O2 tensions had no effect on Na+-K+-ATPase activity or ATP content in pulmonary artery or aortic endothelial cells in culture. However, hyperoxia decreased the fluidity of the plasma membrane of pulmonary artery and aortic endothelial cells in culture, and the time course for the decrease in fluidity parallels that of the hyperoxic inhibition of serotonin transport. These results indicate that hyperoxia decreases fluidity in the hydrophobic core of the plasma membranes of cultured endothelial cells. Such decreases in plasma membrane fluidity may be responsible for hyperoxia-induced alterations in membrane function including decreases in transmembrane transport of amines.

Plasma membrane fluidity in isolated rat hepatocytes: A comparative study using DPH and TMA-DPH as fluorescent probes

1989 ◽  
Vol 4 (3) ◽  
pp. 221-227 ◽  
Author(s):  
ANTONIO BENEDETTI ◽  
GIANNA FERRETTI ◽  
GIOVANNA CURATOLA ◽  
EUGENIO BRUNELLI ◽  
ANNE MARIE JÉZÉQUEL ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Comparative Study ◽  
Membrane Fluidity ◽  
Fluorescent Probes ◽  
Rat Hepatocytes ◽  
Isolated Rat Hepatocytes ◽  
Plasma Membrane Fluidity ◽  
Isolated Rat
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