siRNAs target sites selection of ezrin and the influence of RNA interference on ezrin expression and biological characters of osteosarcoma cells

2012 ◽  
Vol 364 (1-2) ◽  
pp. 363-371 ◽  
Author(s):  
XiFu Shang ◽  
YaoFei Wang ◽  
QiChun Zhao ◽  
KeRong Wu ◽  
Xu Li ◽  
...  
Keyword(s):  
Rna Interference ◽  
Osteosarcoma Cells ◽  
Ezrin Expression ◽  
Target Sites ◽  
Biological Characters ◽  
Selection Of

scholarly journals The integration preference of Sleeping Beauty at non-TA site is related to the transposon end sequences

2019 ◽  
Author(s):  
Yiting Zhou ◽  
Guangwei Ma ◽  
Jiawen Yang ◽  
Yabin Guo
Keyword(s):  
Site Selection ◽  
Genomic Dna ◽  
Consensus Sequence ◽  
Mouse Cell ◽  
Sleeping Beauty ◽  
Consensus Sequences ◽  
Target Site Selection ◽  
Target Sites ◽  
End Sequences ◽  
Selection Of

Abstract Background: Sleeping Beauty (SB) transposon had been thought to strictly integrate into TA dinucleotides. Recently, we found that SB also integrates into non-TA sites at a lower frequency. Here we performed further study on the non-TA integration of SB. Results: 1) SB can integrate into non-TA sites in HEK293T cells as well as in mouse cell lines. 2) Both the hyperactive transposase SB100X and the traditional SB11 catalyze integrations at non-TA sites. 3) The consensus sequence of the non-TA target sites only occur at the opposite side of the sequenced junction between transposon end and the genomic sequences, indicating that the integrations at non-TA sites are mainly aberrant integrations. 4) The consensus sequence of the non-TA target sites is corresponding to the transposon end sequence. When the transposon end sequence is mutated, the consensus sequences changed too. Conclusion: The interaction between the SB transposon end and genomic DNA may be involved in the target site selection of the SB integrations at non-TA sites.

scholarly journals Combinatorial RNA Interference Therapy Prevents Selection of Pre-existing HBV Variants in Human Liver Chimeric Mice

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Yao-Ming Shih ◽  
Cheng-Pu Sun ◽  
Hui-Hsien Chou ◽  
Tzu-Hui Wu ◽  
Chun-Chi Chen ◽  
...  
Keyword(s):  
Rna Interference ◽  
Human Liver ◽  
Chimeric Mice ◽  
Hbv Variants ◽  
Selection Of
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