Genetic analysis of Phn7.1, a major QTL conferring partial resistance to Phytophthora nicotianae in Nicotiana tabacum

2019 ◽  
Vol 39 (1) ◽  
Author(s):  
Justin M. Ma ◽  
Crystal Heim ◽  
Matt Humphry ◽  
J. M. Nifong ◽  
Ramsey S. Lewis
Keyword(s):  
Nicotiana Tabacum ◽  
Genetic Analysis ◽  
Partial Resistance ◽  
Phytophthora Nicotianae ◽  
Major Qtl

scholarly journals RNAseq Reveals Differential Gene Expression Contributing to Phytophthora nicotianae Adaptation to Partial Resistance in Tobacco

Agronomy ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 656
Author(s):  
Jing Jin ◽  
Rui Shi ◽  
Ramsey Steven Lewis ◽  
Howard David Shew
Keyword(s):  
Partial Resistance ◽  
Molecular Mechanisms ◽  
Biological Activities ◽  
Effective Means ◽  
Phytophthora Nicotianae ◽  
Economic Losses ◽  
Black Shank ◽  
Differential Gene ◽  
Go Terms

Phytophthora nicotianae is a devastating oomycete plant pathogen with a wide host range. On tobacco, it causes black shank, a disease that can result in severe economic losses. Deployment of host resistance is one of the most effective means of controlling tobacco black shank, but adaptation to complete and partial resistance by P. nicotianae can limit the long-term effectiveness of the resistance. The molecular basis of adaptation to partial resistance is largely unknown. RNAseq was performed on two isolates of P. nicotianae (adapted to either the susceptible tobacco genotype Hicks or the partially resistant genotype K 326 Wz/Wz) to identify differentially expressed genes (DEGs) during their pathogenic interactions with K 326 Wz/Wz and Hicks. Approximately 69% of the up-regulated DEGs were associated with pathogenicity in the K 326 Wz/Wz-adapted isolate when sampled following infection of its adapted host K 326 Wz/Wz. Thirty-one percent of the up-regulated DEGs were associated with pathogenicity in the Hicks-adapted isolate on K 326 Wz/Wz. A broad spectrum of over-represented gene ontology (GO) terms were assigned to down-regulated genes in the Hicks-adapted isolate. In the host, a series of GO terms involved in nuclear biosynthesis processes were assigned to the down-regulated genes in K 326 Wz/Wz inoculated with K 326 Wz/Wz-adapted isolate. This study enhances our understanding of the molecular mechanisms of P. nicotianae adaptation to partial resistance in tobacco by elucidating how the pathogen recruits pathogenicity-associated genes that impact host biological activities.

Phytophthora nicotianae. [Descriptions of Fungi and Bacteria].

1994 ◽  
Author(s):  
G. Hall
Keyword(s):  
Surface Water ◽  
Nicotiana Tabacum ◽  
Geographical Distribution ◽  
Root Rot ◽  
World Wide ◽  
Phytophthora Nicotianae ◽  
Fruit Rot ◽  
Tomato Root ◽  
Ornamental Crops ◽  
The Tropics

Abstract A description is provided for Phytophthora nicotianae. Information is included on the disease caused by the organism, its transmission, geographical distribution, and hosts. HOSTS: Principally Lycopersicon esculentum, Nicotiana tabacum, Capsicum annuum and Citrus sp. A very large number of other agricultural and ornamental crops, both temperate and tropical, are also affected, including avocado, strawberry, pineapple, papaya, guava, eggplant and durian. DISEASE: Blackshank of tobacco, buckeye of tomato, root and fruit rot of capsicum, root rot of citrus. GEOGRAPHICAL DISTRIBUTION: World-wide, but particularly common in the tropics and sub-tropics. TRANSMISSION: By zoospores in surface water and rainsplash. Chlamydospores (and oospores, when formed) act as perennating structures.

scholarly journals Resistance to Phytophthora nicotianae in Tobacco Breeding Lines Derived from Variety Beinhart 1000

Plant Disease ◽  
2013 ◽  
Vol 97 (2) ◽  
pp. 252-258 ◽  
Author(s):  
Kestrel McCorkle ◽  
Ramsey Lewis ◽  
David Shew
Keyword(s):  
Partial Resistance ◽  
Phytophthora Nicotianae ◽  
Sources Of Resistance ◽  
Black Shank ◽  
Breeding Lines ◽  
Stem Resistance ◽  
Race 1 ◽  
Low Levels ◽  
Dh Lines ◽  
Rapid Emergence

Black shank, caused by Phytophthora nicotianae, is managed primarily by host resistance. The rapid emergence of race 1 eliminated the usefulness of available complete resistance, leading breeders to search for new sources of resistance. Cigar tobacco ‘Beinhart 1000’ (BH) is highly resistant to all races of P. nicotianae. Doubled-haploid (DH) lines from a cross of BH and the susceptible ‘Hicks’ were evaluated for black shank resistance, and quantitative trait loci (QTL) on linkage groups (LGs) 4 and 8 accounted for >43% of the phenotypic variation in resistance. Forty-three DH lines and parents were evaluated, and genotypes with one or both QTL from BH on LGs 4 and 8 had increased incubation periods and decreased root rot but higher final inoculum levels than genotypes with neither QTL. A low level of stem resistance was observed in BH and DH lines with the QTL from BH on LG 4 but not LG 8. Low levels of leaf resistance were seen for Hicks, BH, and DH lines with both QTL from BH on LG 4 and 8. The partial resistance from BH has not been used commercially and may provide an increase in level of partial resistance in future tobacco varieties.

scholarly journals Rootstock-Scion Interaction Affects the Composition and Pathogen Inhibitory Activity of Tobacco (Nicotiana tabacum L.) Root Exudates

Plants ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 1652
Author(s):  
Cheng-Sheng Zhang ◽  
Yanfen Zheng ◽  
Lijuan Peng ◽  
Jianmin Cao
Keyword(s):  
Nicotiana Tabacum ◽  
Root Exudates ◽  
Root Exudate ◽  
Phytophthora Nicotianae ◽  
Susceptible Cultivar ◽  
Black Shank ◽  
Nicotiana Tabacum L ◽  
Disease Induction ◽  
Resistant Rootstock ◽  
Ethylhexyl Phthalate

The composition and allelopathy to Phytophthora nicotianae (the causal agent of tobacco black shank disease) of root exudates from a resistant tobacco (Nicotiana tabacum L.) cultivar Gexin 3, a susceptible cultivar Xiaohuangjin 1025 and their reciprocal grafts were investigated. Grafting with disease-resistant rootstock could improve resistance to black shank; this is closely related to the allelopathy of root exudates. The root exudates from the resistant cultivar inhibited the growth of P. nicotianae, while those from the susceptible cultivar promoted the growth; the grafting varieties had intermediate properties. The root exudate composition differed among cultivars. Gexin 3 was rich in esters and fatty acids, while Xiaohuangjin 1025 contained more hydrocarbons and phenolic acids. The composition of root exudates of grafted cultivars as well as their allelopathy to P. nicotianae were altered, and tended to be close to the composition of cultivar used as rootstock. Eugenol, 4-tert-butylphenol, mono (2-ethylhexyl) phthalate, 4-hydroxybenzoic acid, 2,6-di-tert-butylphenol, dipropyl phthalate, and methyl myristate were identified as the main compounds contributing to inhibitory properties of root exudates. Sorbitol was suggested to play a role in disease induction. Overall, rootstock–scion interaction affected the composition of tobacco root exudates, which may be attributed to the different disease resistance among grafted plants, rootstock and scion.

Genetic analysis of partial resistance to vine decline in watermelon [Citrullus lanatus (Thunb.) Matsum&Nakai]

2020 ◽  
Vol 73 (2) ◽  
pp. 361-365
Author(s):  
Siddharood Maragal ◽  
K. Arpitha ◽  
Muttanna Revadi ◽  
Sourav Mahapatra ◽  
K. N. Avinash ◽  
...  
Keyword(s):  
Genetic Analysis ◽  
Partial Resistance ◽  
Citrullus Lanatus ◽  
Vine Decline

Genetic Analysis for Partial Resistance to an Iranian Strain of Bacterial Leaf Streak (X. campestris pv. hordei) in Barley

1994 ◽  
Vol 113 (4) ◽  
pp. 323-326 ◽  
Author(s):  
A. Alizadeh ◽  
V. Benetti ◽  
A. Sarrafi ◽  
G. Barrault ◽  
L. Albertini
Keyword(s):  
Genetic Analysis ◽  
Partial Resistance ◽  
Bacterial Leaf Streak

Genetic analysis of partial resistance to coffee leaf rust (Hemileia vastatrix Berk & Br.) introgressed into the cultivated Coffea arabica L. from the diploid C. canephora species

Euphytica ◽  
2008 ◽  
Vol 167 (1) ◽  
pp. 57-67 ◽  
Author(s):  
Juan Carlos Herrera P. ◽  
Gabriel Alvarado A. ◽  
Hernando A. Cortina G. ◽  
Marie-Christine Combes ◽  
Gladys Romero G. ◽  
...  
Keyword(s):  
Genetic Analysis ◽  
Leaf Rust ◽  
Partial Resistance ◽  
Coffea Arabica ◽  
Hemileia Vastatrix ◽  
Coffee Leaf Rust ◽  
Coffea Arabica L

scholarly journals A Rapid Technique for Determination of Races of Phytophthora nicotianae on Tobacco

Plant Disease ◽  
2007 ◽  
Vol 91 (8) ◽  
pp. 985-989 ◽  
Author(s):  
Walter A. Gutiérrez ◽  
Asimina L. Mila
Keyword(s):  
North Carolina ◽  
Tissue Culture ◽  
Nicotiana Tabacum ◽  
Phytophthora Nicotianae ◽  
Breeding Lines ◽  
Laboratory Technique ◽  
Different Types ◽  
Differential Cultivars ◽  
Tobacco Seedlings

A laboratory technique for determining races of Phytophthora nicotianae on tobacco (Nicotiana tabacum) was developed and compared with a commonly used greenhouse method. The laboratory technique was based on production and inoculation of tobacco seedlings in tissue culture plates. Three P. nicotianae isolates from North Carolina previously determined as race 0 and 1 were used. Four tobacco cultivars and two breeding lines with different types of resistance were used as differential cultivars: K-326, K-346, NC-71, NC-1071, L8, and Ky14xL8. Plants were evaluated 7 and 14 days after inoculation. Five differential cultivars (K-326, K-346, NC-1071, NC-71, and L8) were determined to be sufficient to differentiate races 0 and 1. Cv. Ky14xL8 was ineffective for differentiation of races and produced inconsistent results. The laboratory technique was as effective as the greenhouse technique for distinguishing different races of P. nicotianae for every isolate in all experiments. Additionally, the most reliable results for both methods were obtained when evaluations were made 14 days after inoculation. The laboratory technique was validated with 21 isolates collected from four counties in North Carolina. The laboratory technique produced results 2 weeks faster than the greenhouse technique and required significantly less space and labor than the greenhouse technique for the same number of isolates. Additionally, the larger number of seedlings used in the laboratory technique increased the robustness of the results, especially for isolates for which race identification was unclear with the greenhouse technique. We propose that the laboratory technique has the potential for evaluation of tobacco resistance in other pathosystems as well.

GENETIC ANALYSIS OF INHERITANCE OF PARTIAL RESISTANCE TO FUSARIUM OXYSPORUM IN ASIATIC HYBRIDS OF LILY USING RAPD MARKERS

1996 ◽  
pp. 209-218 ◽  
Author(s):  
Th.P. Straathof ◽  
J.M. Van Tuyl ◽  
B. Dekker ◽  
M.J.M. Van Winden ◽  
J.M. Sandbrink
Keyword(s):  
Genetic Analysis ◽  
Fusarium Oxysporum ◽  
Partial Resistance ◽  
Rapd Markers ◽  
Asiatic Hybrids
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