Development, characterization, and in vitro evaluation of phosphatidylcholine–sodium cholate-based nanoparticles for siRNA delivery to MCF-7 human breast cancer cells

2015 ◽  
Vol 17 (3) ◽  
Author(s):  
Sebastián Ezequiel Pérez ◽  
Yamila Gándola ◽  
Adriana Mónica Carlucci ◽  
Lorena González
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Human Breast Cancer ◽  
Breast Cancer Cells ◽  
Sirna Delivery ◽  
Sodium Cholate ◽  
Human Breast Cancer Cells ◽  
Human Breast ◽  
Mcf 7

scholarly journals Dioscorealide B from the Traditional Thai Medicine Hua-Khao-Yen Induces Apoptosis in MCF-7 Human Breast Cancer Cells via Modulation of Bax, Bak and Bcl-2 Protein Expression

2010 ◽  
Vol 5 (12) ◽  
pp. 1934578X1000501
Author(s):  
Jiraporn Saekoo ◽  
Potchanapond Graidist ◽  
Wilairat Leeanansaksiri ◽  
Chavaboon Dechsukum ◽  
Arunporn Itharat
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Human Breast Cancer ◽  
Breast Cancer Cells ◽  
Human Breast Cancer Cells ◽  
Human Breast ◽  
Antitumor Property ◽  
Pharmacologically Active ◽  
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Dioscorealide B is a pharmacologically active compound from the rhizome of the Thai medicinal plant Dioscorea membranacea. Here, we demonstrated that in vitro treatment of dioscorealide B resulted in a cytotoxic effect on MCF-7 human breast cancer cells (IC50 = 2.82 μM). To determine whether this compound induces apoptosis in MCF-7, the Annexin V assay was performed. The data showed that the number of apoptotic cells were increased 7–12 folds over that of the control cells after treatment with various concentrations of dioscorealide B (3, 6 and 12 μM) for 24 hours. Dioscorealide B-induced apoptosis was associated with modulation of the multidomain Bcl-2 family members Bax, Bak and Bcl-2. After treatment with 3 μM dioscorealide B, acceleration of the level of proapoptotic proteins Bax and Bak were observed at 6 hours and 12 hours, respectively, while the decrease in the expression of antiapoptotic protein Bcl-2 was observed 3 hours after the treatment. These effects of dioscorealide B might result in the activation of caspase-8, -9 and -7, which lead to apoptosis in MCF-7 cells. Taken together, the results of this study provide evidence that dioscorealide B possesses an antitumor property against human breast cancer cells and thus provide the molecular basis for the further development of dioscorealide B as a novel chemotherapeutic agent for breast cancer treatment.

Combined effects of Buserelin, estradiol and Tamoxifen on the growth of MCF-7 human breast cancer cells in vitro

1986 ◽  
Vol 140 (2) ◽  
pp. 550-556 ◽  
Author(s):  
J.A. Foekens ◽  
M.S. Henkelman ◽  
J.F. Fukkink ◽  
M.A. Blankenstein ◽  
J.G.M. Klijn
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Human Breast Cancer ◽  
Breast Cancer Cells ◽  
Combined Effects ◽  
Human Breast Cancer Cells ◽  
Human Breast ◽  
Mcf 7

A new methyl-thiochroman-4-ketone derivative induces apoptosis in MCF-7 human breast cancer cells <italic>in vitro</italic>

2012 ◽  
Vol 57 (8) ◽  
pp. 626-633
Author(s):  
YuXin LIU ◽  
Xin HUANG ◽  
LiMin SU ◽  
GengLiang YANG ◽  
DeZhong HAO ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Human Breast Cancer ◽  
Breast Cancer Cells ◽  
Human Breast Cancer Cells ◽  
Human Breast ◽  
Mcf 7
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