scholarly journals Stability Kinetics of Influenza Vaccine Coated onto Microneedles During Drying and Storage

2010 ◽  
Vol 28 (1) ◽  
pp. 135-144 ◽  
Author(s):  
Yeu-Chun Kim ◽  
Fu-Shi Quan ◽  
Richard W. Compans ◽  
Sang-Moo Kang ◽  
Mark R. Prausnitz
Keyword(s):  
Influenza Vaccine ◽  
Stability Kinetics ◽  
And Storage ◽  
Kinetics Of

scholarly journals Growth kinetics of Salmonella Typhimurium and Listeria monocytogenes in buffalo milk under different processing and storage conditions

2021 ◽  
Vol 51 (11) ◽  
Author(s):  
Joelson Sousa Lima ◽  
Ana Paula Presley Oliveira Sampaio ◽  
Mylla Christy da Silva Dufossé ◽  
Paula Fernanda Morais de Sousa ◽  
Josyane Brasil da Silva ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Salmonella Typhimurium ◽  
Growth Kinetics ◽  
Storage Conditions ◽  
Buffalo Milk ◽  
Production Chain ◽  
Pasteurized Milk ◽  
Processing And Storage ◽  
And Storage ◽  
Kinetics Of

ABSTRACT: Buffalo milk is rich in nutrients and can serve as a substrate for the proliferation of microorganisms. Thus, the objective of the present study was to evaluate the growth kinetics of Salmonella Typhimurium and Listeria monocytogenes in buffalo milk under different processing and storage conditions. Samples of raw and pasteurized milk were inoculated with 1 CFU of each bacterium, separately and together, per 25 mL. After contamination, samples were stored at 8 °C or 37 °C, and bacterial counts were performed at 24, 48, and 168 h. In addition, the accompanying microbiota growth, pH, and the effect of these variables on the growth kinetics of microorganisms were monitored. The pathogens tested were able to proliferate under most conditions tested, reaching high titers throughout the experimental period. At 37 °C, there was a decrease in pH and an increase in the accompanying microbiota that interfered with the microbial growth curve. It was also observed that pasteurized milk subjected to 8 °C provided better conditions for the multiplication of bacteria. Therefore, it was concluded that care throughout the production chain, storage, and commercialization of milk must be adopted to guarantee the microbiological safety of this food.

scholarly journals Gas Sensing by Bacterial H-NOX Proteins: An MD Study

Molecules ◽  
2020 ◽  
Vol 25 (12) ◽  
pp. 2882 ◽  
Author(s):  
Ahmed M. Rozza ◽  
Dóra K. Menyhárd ◽  
Julianna Oláh
Keyword(s):  
Gas Sensing ◽  
Hydrophobic Interactions ◽  
Transport Systems ◽  
Alternative Mode ◽  
Channel System ◽  
Gas Molecule ◽  
O2 Diffusion ◽  
Dynamics Simulations ◽  
And Storage ◽  
Kinetics Of

Gas sensing is crucial for both prokaryotes and eukaryotes and is primarily performed by heme-based sensors, including H-NOX domains. These systems may provide a new, alternative mode for transporting gaseous molecules in higher organisms, but for the development of such systems, a detailed understanding of the ligand-binding properties is required. Here, we focused on ligand migration within the protein matrix: we performed molecular dynamics simulations on three bacterial (Ka, Ns and Cs) H-NOX proteins and studied the kinetics of CO, NO and O2 diffusion. We compared the response of the protein structure to the presence of ligands, diffusion rate constants, tunnel systems and storage pockets. We found that the rate constant for diffusion decreases in the O2 > NO > CO order in all proteins, and in the Ns > Ks > Cs order if single-gas is considered. Competition between gases seems to seriously influence the residential time of ligands spent in the distal pocket. The channel system is profoundly determined by the overall fold, but the sidechain pattern has a significant role in blocking certain channels by hydrophobic interactions between bulky groups, cation–π interactions or hydrogen bonding triads. The majority of storage pockets are determined by local sidechain composition, although certain functional cavities, such as the distal and proximal pockets are found in all systems. A major guideline for the design of gas transport systems is the need to chemically bind the gas molecule to the protein, possibly joining several proteins with several heme groups together.

scholarly journals Combustion Characteristics of Hydrochar and Pyrochar Derived from Digested Sewage Sludge

Energies ◽  
2020 ◽  
Vol 13 (16) ◽  
pp. 4164 ◽  
Author(s):  
Pablo J. Arauzo ◽  
María Atienza-Martínez ◽  
Javier Ábrego ◽  
Maciej P. Olszewski ◽  
Zebin Cao ◽  
...  
Keyword(s):  
Sewage Sludge ◽  
Reaction Pathways ◽  
Energy Yield ◽  
Combustion Kinetics ◽  
Coal Blending ◽  
Carbon Recovery ◽  
And Storage ◽  
Kinetics Of ◽  
Digested Sewage Sludge ◽  
Lower Carbon

In this paper, hydrochars and pyrochars were produced at 260 °C under different residence times (2 and 4 h) using anaerobic digested sewage sludge (SSL) as initial feedstock. The effect of reaction time on the fuel properties of hydrochars and pyrochars was evaluated. Moreover, the combustion kinetics of raw SSL and the derived pyrochars and hydrochars without coal blending were determined at two different air flows (20 and 90 mL/min) and compared. In the same conditions, the yield of hydrochar was significantly lower than that of pyrochar, confirming the different reaction pathways followed in each process. The results showed hydrochars have lower carbon recovery and energy yield than pyrochars, making the latter more suitable for energy purposes. The thermogravimetric combustion study showed that both thermochemical treatments increased the ignition temperature but decreased the burnout temperature, which results in higher stability during handling and storage. However, raw SSL is better for combustion than hydrochar according to the combustibility index. In addition, the kinetic study showed that the activation energy of the combustion of biochars, especially pyrochar, is lower than that of raw SSL, which is advantageous for their combustion.

Kinetics of viral shedding and immune responses in adults following administration of cold-adapted influenza vaccine

Vaccine ◽  
2009 ◽  
Vol 27 (52) ◽  
pp. 7359-7366 ◽  
Author(s):  
Laura L. Hammitt ◽  
Julie Patterson Bartlett ◽  
Shaobing Li ◽  
Jeremy Rahkola ◽  
Nancy Lang ◽  
...  
Keyword(s):  
Immune Responses ◽  
Influenza Vaccine ◽  
Cold Adapted ◽  
Viral Shedding ◽  
Kinetics Of

Reverse Stability Kinetics of Meat Pigment Oxidation in Aqueous Extract from Fresh Beef

2017 ◽  
Vol 82 (12) ◽  
pp. 2910-2914 ◽  
Author(s):  
John C. Frelka ◽  
David M. Phinney ◽  
Macdonald P. Wick ◽  
Dennis R. Heldman
Keyword(s):  
Aqueous Extract ◽  
Stability Kinetics ◽  
Kinetics Of

Use of whey permeate containing in situ synthesised galacto-oligosaccharides for the growth and preservation of Lactobacillus plantarum

2013 ◽  
Vol 80 (3) ◽  
pp. 374-381 ◽  
Author(s):  
Marina Golowczyc ◽  
Carlos Vera ◽  
Mauricio Santos ◽  
Cecilia Guerrero ◽  
Paula Carasi ◽  
...  
Keyword(s):  
Lactobacillus Plantarum ◽  
Culture Media ◽  
Enzymatic Reaction ◽  
Raw Material ◽  
Whey Permeate ◽  
Cheese Industry ◽  
Potential Impact ◽  
And Storage ◽  
Kinetics Of

Galacto-oligosaccharides (GOS) are prebiotics that have a beneficial effect on human health by promoting the growth of probiotic bacteria in the gut. GOS are commonly produced from lactose in an enzymatic reaction catalysed by β-galactosidase, named transglycosylation. Lactose is the main constituent of whey permeate (WP), normally wasted output from the cheese industry. Therefore, the main goal of this work was to optimise the synthesis of GOS in WP using β-galatosidase from Aspergillus oryzaea. WP and whey permeate enzymatically treated (WP-GOS) were used as culture media of Lactobacillus plantarum 299v. Lb. plantarum 299v attained the stationary phase in approximately 16 h, reaching 3·6 and 4·1×108 CFU/ml in WP and WP-GOS, respectively. The in situ synthesised GOS were not consumed during growth. No significant differences were observed in the growth kinetics of microorganisms in both media. After fermentation, microorganisms were dehydrated by freeze-drying and spray-drying and stored. The recovery of microorganisms after fermentation, dehydration and storage at 4 °C for at least 120 d was above 108 CFU/g. These studies demonstrated that WP is an appropriate substrate for the synthesis of GOS and the obtained product is also adequate as culture medium of Lb. plantarum 299v. The coexistence of GOS and dehydrated viable probiotic microorganisms, prepared using an effluent as raw material, represents the main achievement of this work, with potential impact in the development of functional foods.

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