Multiple and integrated functions of floral C-class MADS-box genes in flower and fruit development of Physalis floridana

2021 ◽  
Author(s):  
Jing Zhao ◽  
Pichang Gong ◽  
Hongyan Liu ◽  
Mingshu Zhang ◽  
Chaoying He
Keyword(s):  
Fruit Development ◽  
Mads Box ◽  
Mads Box Genes ◽  
Flower And Fruit Development

The Role of MADS-Box Genes in the Control of Flower and Fruit Development in Arabidopsis

2003 ◽  
pp. 20-27
Author(s):  
Soraya Pelaz ◽  
Sarah Liljegren ◽  
Adrienne Roeder ◽  
Cristina Ferrándiz ◽  
Anusak Pinyopich ◽  
...  
Keyword(s):  
Fruit Development ◽  
Mads Box ◽  
Mads Box Genes ◽  
Flower And Fruit Development

Genome-wide identification and analysis of the MADS-box gene family and its potential role in fruit ripening in black raspberry (Rubus occidentalis L.)

2021 ◽  
pp. 1-15
Author(s):  
Yaqiong Wu ◽  
Chunhong Zhang ◽  
Wenlong Wu ◽  
Weilin Li ◽  
Lianfei Lyu
Keyword(s):  
Gene Family ◽  
Fruit Ripening ◽  
Fruit Development ◽  
Expression Patterns ◽  
Type I ◽  
Black Raspberry ◽  
Mads Box ◽  
Mads Box Genes ◽  
Genome Wide ◽  
Mads Box Gene

BACKGROUND: Black raspberry is a vital fruit crop with a high antioxidant function. MADS-box genes play an important role in the regulation of fruit development in angiosperms. OBJECTIVE: To understand the regulatory role of the MADS-box family, a total of 80 MADS-box genes were identified and analyzed. METHODS: The MADS-box genes in the black raspberry genome were analyzed using bioinformatics methods. Through an analysis of the promoter elements, the possible functions of different members of the family were predicted. The spatiotemporal expression patterns of members of the MADS-box family during black raspberry fruit development and ripening were systematically analyzed. RESULTS: The genes were classified into type I (Mα: 33; Mβ: 6; Mγ: 10) and type II (MIKC *: 2; MIKCC: 29) genes. We also obtained a complete overview of the RoMADS-box gene family through phylogenetic, gene structure, conserved motif, and cis element analyses. The relative expression analysis showed different expression patterns, and most RoMADS-box genes were more highly expressed in fruit than in other tissues of black raspberry. CONCLUSIONS: This finding indicates that the MADS-box gene family is involved in the regulation of fruit ripening processes in black raspberry.

scholarly journals Cloning and Characterization of Five MADS Box Genes in Peach (Prunus persica)

HortScience ◽  
2005 ◽  
Vol 40 (4) ◽  
pp. 1146D-1146
Author(s):  
Yong Xu ◽  
Fan Wu ◽  
Rong-Cai Ma
Keyword(s):  
Expression Pattern ◽  
Prunus Persica ◽  
Pcr Analysis ◽  
Mads Box ◽  
Mads Box Genes ◽  
Rt Pcr ◽  
Coding Regions ◽  
Development Processes ◽  
Flower And Fruit Development ◽  
Inner Parts

MADS box genes regulate most of the development processes in plants. Studying peach MADS box genes will provide insights into its flower and fruit development. Five MADS box cDNAs with complete coding regions were cloned in this study. PpMADS2 cDNA is 1116-bp long. RT-PCR analysis indicated that PpMADS2 is expressed in leaf, flower, fruit, and nutlet. PpMAD4 cDNA is 824-bp long, which is the homologue of Agamous. RT-PCR analysis indicated that PpMADS4 is expressed in the two inner parts of flower, fruit, and nutlet; and was absent in leaf and the two outer parts of the flower. This expression pattern is similar to that of Agamous gene in A. thaliana. PpMADS4 could promote the flowering process in A. thaliana tested by genetic transformation. PpMADS5 cDNA is 873-bp long, which is the homologue of SEP3. RT-PCR analysis indicated that PpMADS5 is expressed in the three inner parts of flower, fruit, and nutlet; and was absent in leaf and sepal, similar to the expression pattern of SEP3 gene in Arabidopsis. PpMAD6 cDNA is 1037-bp long, which is the homologue of FUL. RT-PCR analysis indicated that PpMADS6 is expressed in leaf, sepal, petal, carpel, and fruit; and was absent in stamen and nutlet. PpMAD7 cDNA is 1147-bp long, which is the homologue of SEP1. RT-PCR analysis indicated that PpMADS7 is expressed in the four parts of flower and fruit, and was absent in leaf, stamen, and nutlet. Furthermore, two SSRs were identified in the 5' UTR in the two MADS box genes, PpMADS2 and PpMADS7, respectively. The SSR in PpMAD2 was more polymorphic than that in PpMADS7 in the 39 Prunus accessions collected.

scholarly journals Characteristics of banana B genome MADS-box family demonstrate their roles in fruit development, ripening, and stress

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Yunke Zheng ◽  
Mengting Liu ◽  
Caihong Jia ◽  
Jingyi Wang ◽  
Biyu Xu ◽  
...  
Keyword(s):  
Fruit Development ◽  
Type I ◽  
Evolutionary Analysis ◽  
Banana Fruit ◽  
Type Ii ◽  
Mads Box ◽  
Mads Box Genes ◽  
Systematic Analysis ◽  
Musa Balbisiana ◽  
B Genome

AbstractMADS-box genes are critical regulators of growth and development in flowering plants. Sequencing of the Musa balbisiana (B) genome has provided a platform for the systematic analysis of the MADS-box gene family in the important banana ancestor Musa balbisiana. Seventy-seven MADS-box genes, including 18 type I and 59 type II, were strictly identified from the banana (Pisang Klutuk Wulung, PKW, 2n = 2x = 22) B genome. These genes have been preferentially placed on the banana B genome. Evolutionary analysis suggested that M. balbisiana MCM1-AGAMOUS-DEFICIENS-SRF (MbMADS) might be organized into the MIKCc, MIKC*, Mα, Mβ, and Mγ groups according to the phylogeny. MIKCc was then further categorized into 10 subfamilies according to conserved motif and gene structure analyses. The well-defined MADS-box genes highlight gene birth and death in banana. MbMADSes originated from the same ancestor as MaMADSes. Transcriptome analysis in cultivated banana (ABB) revealed that MbMADSes were conserved and differentially expressed in several organs, in various fruit developing and ripening stages, and in stress treatments, indicating the participation of these genes in fruit development, ripening, and stress responses. Of note, SEP/AGL2 and AG, as well as other several type II MADS-box genes, including the STMADS11 and TM3/SOC1 subfamilies, indicated elevated expression throughout banana fruit development, ripening, and stress treatments, indicating their new parts in controlling fruit development and ripening. According to the co-expression network analysis, MbMADS75 interacted with bZIP and seven other transcription factors to perform its function. This systematic analysis reveals fruit development, ripening, and stress candidate MbMADSes genes for additional functional studies in plants, improving our understanding of the transcriptional regulation of MbMADSes genes and providing a base for genetic modification of MADS-mediated fruit development, ripening, and stress.

Overexpression of SlMBP22 in Tomato Affects Flower Morphology, Fruit Set and Development

2021 ◽  
Author(s):  
Fenfen Li ◽  
Yanhua Jia ◽  
Xinyu Chen ◽  
Shengen Zhou ◽  
Qiaoli Xie ◽  
...  
Keyword(s):  
Fruit Development ◽  
Pollen Viability ◽  
Reproductive Development ◽  
Reproductive Organs ◽  
Homeotic Genes ◽  
Mads Box ◽  
Flower And Fruit Development ◽  
Cross Fertilization ◽  
Two Hybrid ◽  
Floral Homeotic

Abstract MADS-domain transcription factors have been clarified as key regulators involved in proper flower and fruit development in angiosperms. Bs genes, as members of the MADS-box subfamily, have been suggested to play an important role during the evolution of the reproductive organs in seed plants. Our knowledge about their effects on reproductive development in fruit crops like tomato (Solanum lycopersicum), however, is still unclear. Here, we found that the overexpression of SlMBP22 (SlMBP22-OE) resulted in considerable alterations regarding floral morphology, and affected the expression levels of several floral homeotic genes. Further analysis by yeast-two-hybrid assays demonstrated that SlMBP22 could form dimers with class A protein MACROCALYX (MC) and with SEPALLATA (SEP) floral homeotic proteins TM5 and TM29, respectively. In addition, pollen viability and cross-fertilization assays suggested that the defect in female reproductive development was responsible for infertility phenotype observed in the strong overexpression transgenic plants. The mild overexpression transgenic fruits were reduced in size, as a result of reduced cell expansion, rather than impaired cell division. Additionally, overexpression of SlMBP22 in tomato not only affected proanthocyanidin (PA) accumulation but also altered seed dormancy. Taken together, these findings may provide new insights into the knowledge of Bs MADS-box genes in flower and fruit development in tomato.

Characterization of 10 MADS-box genes from Pyrus pyrifolia and their differential expression during fruit development and ripening

Gene ◽  
2013 ◽  
Vol 528 (2) ◽  
pp. 183-194 ◽  
Author(s):  
Benjamin Ewa Ubi ◽  
Takanori Saito ◽  
Songling Bai ◽  
Chikako Nishitani ◽  
Yusuke Ban ◽  
...  
Keyword(s):  
Differential Expression ◽  
Fruit Development ◽  
Pyrus Pyrifolia ◽  
Mads Box ◽  
Mads Box Genes ◽  
Fruit Development And Ripening

Variations on a theme in fruit development: the PLE lineage of MADS-box genes in tomato (TAGL1) and other species

Planta ◽  
2017 ◽  
Vol 246 (2) ◽  
pp. 313-321 ◽  
Author(s):  
Danielle C. Garceau ◽  
Megan K. Batson ◽  
Irvin L. Pan
Keyword(s):  
Fruit Development ◽  
Mads Box ◽  
Mads Box Genes ◽  
Variations On A Theme

Isolation of the three grape sub-lineages of B-class MADS-box TM6, PISTILLATA and APETALA3 genes which are differentially expressed during flower and fruit development

Gene ◽  
2007 ◽  
Vol 404 (1-2) ◽  
pp. 10-24 ◽  
Author(s):  
María Josefina Poupin ◽  
Fernán Federici ◽  
Consuelo Medina ◽  
José Tomás Matus ◽  
Tania Timmermann ◽  
...  
Keyword(s):  
Fruit Development ◽  
Differentially Expressed ◽  
Mads Box ◽  
Flower And Fruit Development

scholarly journals Genome-Wide Analysis of the MADS-Box Transcription Factor Family in Solanum lycopersicum

2019 ◽  
Vol 20 (12) ◽  
pp. 2961 ◽  
Author(s):  
Yunshu Wang ◽  
Jianling Zhang ◽  
Zongli Hu ◽  
Xuhu Guo ◽  
Shibing Tian ◽  
...  
Keyword(s):  
Gene Family ◽  
Fruit Development ◽  
Expression Patterns ◽  
Functional Characterization ◽  
Constitutive Expression ◽  
Floral Organ ◽  
Mads Box ◽  
Mads Box Genes ◽  
Fruit Development And Ripening ◽  
Mads Box Gene

MADS-box family genes encode transcription factors that are involved in multiple developmental processes in plants, especially in floral organ specification, fruit development, and ripening. However, a comprehensive analysis of tomato MADS-box family genes, which is an important model plant to study flower fruit development and ripening, remains obscure. To gain insight into the MADS-box genes in tomato, 131 tomato MADS-box genes were identified. These genes could be divided into five groups (Mα, Mβ, Mγ, Mδ, and MIKC) and were found to be located on all 12 chromosomes. We further analyzed the phylogenetic relationships among Arabidopsis and tomato, as well as the protein motif structure and exon–intron organization, to better understand the tomato MADS-box gene family. Additionally, owing to the role of MADS-box genes in floral organ identification and fruit development, the constitutive expression patterns of MADS-box genes at different stages in tomato development were identified. We analyzed 15 tomato MADS-box genes involved in floral organ identification and five tomato MADS-box genes related to fruit development by qRT-PCR. Collectively, our study provides a comprehensive and systematic analysis of the tomato MADS-box genes and would be valuable for the further functional characterization of some important members of the MADS-box gene family.

scholarly journals 092 RNA Localization of a Strawberry MADS-Box Gene (SAG1) Involved in Fruit Development

HortScience ◽  
1999 ◽  
Vol 34 (3) ◽  
pp. 457C-457 ◽  
Author(s):  
Faye M. Rosin ◽  
David Hannapel
Keyword(s):  
Fruit Development ◽  
Sequence Similarity ◽  
Floral Organ ◽  
Reproductive Organ ◽  
Amino Acid Sequence Similarity ◽  
Nucleotide Sequence Analysis ◽  
Mads Box ◽  
Mads Box Genes ◽  
Mads Box Gene ◽  
And Control

MADS-box genes are an important family of highly conserved regulatory genes in plants, animals, and yeast. Genetic analyses have shown that plant MADS-box genes are homeotic and control both the spatial and temporal location of specific organs. While MADS-box genes have been extensively studied and characterized in floral organ development, their involvement in other developmental processes, such as fruit development, is not well understood. From a strawberry fruit cDNA library, we have identified a strawberry AGAMOUS-like MADS-box gene (SAG1) that is expressed in developing fruit, but not in leaves. This is the first MADS-box gene to be isolated from strawberry. The hypothesis guiding this research is that SAG1 plays an important role in the development of the fruit. Nucleotide sequence analysis showed that this cDNA had the highest sequence match to genes from the AGAMOUS family. Comparison of amino acid sequence similarity between SAG1 and members of this family ranged from 70 to 75% overall, and between 98% to100% within the MADS-box. Involvement in stamen and carpel identity is one function of this family of MADS-box genes. Northern hybridizations were performed in order to analyze the expression of this gene at the RNA level. RNA was extracted from various organs of Fragaria ×ananassa, c.v. Calypso. SAG1 RNA expression was specific to stamens, carpels and all stages of fruit and seed development. No expression was detected in roots, leaves, or sepals. Thus, we conclude that SAG1 RNA is involved in reproductive organ and fruit development.

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