Enhancement of diepoxin ζ production with in situ resin adsorption in mycelial liquid culture of the endophytic fungus Berkleasmium sp. Dzf12 from Dioscorea zingiberensis

2011 ◽  
Vol 27 (12) ◽  
pp. 2753-2758 ◽  
Author(s):  
Jianglin Zhao ◽  
Yan Li ◽  
Tijiang Shan ◽  
Yan Mou ◽  
Ligang Zhou
Keyword(s):  
Endophytic Fungus ◽  
Liquid Culture ◽  
Dioscorea Zingiberensis ◽  
Resin Adsorption ◽  
Diepoxin Ζ

scholarly journals Spirobisnaphthalenes from the Endophytic Fungus Dzf12 of Dioscorea zingiberensis and Their Antimicrobial Activities

2009 ◽  
Vol 4 (11) ◽  
pp. 1934578X0900401 ◽  
Author(s):  
Xiaoyue Cai ◽  
Tijiang Shan ◽  
Peiqin Li ◽  
Yongfu Huang ◽  
Lijian Xu ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Medicinal Plant ◽  
Endophytic Fungus ◽  
Antimicrobial Activities ◽  
Spectrometric Analysis ◽  
Antifungal Activities ◽  
Dioscorea Zingiberensis ◽  
Antibacterial And Antifungal Activities ◽  
Antibacterial And Antifungal ◽  
Diepoxin Ζ

Five spirobisnaphthalenes, namely palmarumycin CP17 (1), diepoxin κ (2), diepoxin η (3), diepoxin ζ (4), and diepoxin γ (5), were isolated from the endophytic fungus Dzf12 associated with the medicinal plant Dioscorea zingiberensis C. H. Wright. Their structures were identified by physicochemical and spectrometric analysis. Among these spirobisnaphthalenes, 2 was found to have antibacterial activity, and the mixture of 3 and 4 was detected to have both antibacterial and antifungal activities.

scholarly journals Enhancement of Diosgenin Production in Dioscorea zingiberensis Cell Culture by Oligosaccharide Elicitor from its Endophytic Fungus Fusarium oxysporum Dzf17

2009 ◽  
Vol 4 (11) ◽  
pp. 1934578X0900401
Author(s):  
Ruifen Zhang ◽  
Peiqin Li ◽  
Lijian Xu ◽  
Yuanquan Chen ◽  
Peng Sui ◽  
...  
Keyword(s):  
Suspension Culture ◽  
Cell Suspension ◽  
Fusarium Oxysporum ◽  
Cell Suspension Culture ◽  
Endophytic Fungus ◽  
Cultured Cells ◽  
Cell Suspension Cultures ◽  
Fungal Cell ◽  
Partial Acid Hydrolysis ◽  
Dioscorea Zingiberensis

Diosgenin accumulation in cell suspension cultures of Dioscorea zingiberensis C. H. Wright was enhanced by treatment with saccharide elicitors from its endophytic fungus Fusarium oxysporum isolate Dzf17. The crude oligosaccharide was prepared by partial acid hydrolysis of the isolated Dzf17 fungal cell wall fragments. Optimal elicitation of diosgenin production by the isolated Dzf17 oligosaccharide in cell suspension culture was achieved when the oligosaccharide was added to the medium at a concentration of 30 mg/L after 16-day's continuous cell suspension culture, and the cells were cultured for another 8 days before harvesting. By using these optimal conditions, the diosgenin yield of the cultured cells reached its maximum of 5.25 mg/L, which was over a three-fold increase.

scholarly journals Identification and in situ removal of an inhibitory intermediate to develop an efficient phytosterol bioconversion process using a cyclodextrin-resting cell system

RSC Advances ◽  
2021 ◽  
Vol 11 (40) ◽  
pp. 24787-24793
Author(s):  
Da Wang ◽  
Jian Zhang ◽  
Dan-Dan Cao ◽  
Xuedong Wang ◽  
Dongzhi Wei
Keyword(s):  
Production Efficiency ◽  
Cell System ◽  
Resting Cell ◽  
Commercial Scale ◽  
System P ◽  
Resin Adsorption

We identified an inhibitory intermediate, 4-ene-3-keto steroids, that limits the bioconversion rate and provided a solution based on resin adsorption for improving 9α-OH-AD production efficiency in a commercial-scale process.

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