Production and cell surface display of recombinant anthrax protective antigen on the surface layer of attenuated Bacillus anthracis

2014 ◽  
Vol 31 (2) ◽  
pp. 345-352 ◽  
Author(s):  
Yan-chun Wang ◽  
Sheng-ling Yuan ◽  
Hao-xia Tao ◽  
Ling-chun Wang ◽  
Zhao-shan Zhang ◽  
...  
Keyword(s):  
Surface Layer ◽  
Cell Surface ◽  
Bacillus Anthracis ◽  
Protective Antigen ◽  
Surface Display ◽  
Cell Surface Display ◽  
Anthrax Protective Antigen

scholarly journals Cell Surface-Exposed Tetanus Toxin Fragment C Produced by Recombinant Bacillus anthracis Protects against Tetanus Toxin

1999 ◽  
Vol 67 (9) ◽  
pp. 4847-4850 ◽  
Author(s):  
Stéphane Mesnage ◽  
Martine Weber-Levy ◽  
Michel Haustant ◽  
Michèle Mock ◽  
Agnès Fouet
Keyword(s):  
Cell Surface ◽  
Bacillus Anthracis ◽  
Tetanus Toxin ◽  
Surface Display ◽  
Humoral Response ◽  
Cell Surface Display ◽  
Hybrid Protein ◽  
A Cell ◽  
Component Gene

Bacillus anthracis, the causal agent of anthrax, synthesizes two surface layer (S-layer) proteins, EA1 and Sap, which account for 5 to 10% of total protein and are expressed in vivo. A recombinant B. anthracis strain was constructed by integrating into the chromosome a translational fusion harboring the DNA fragments encoding the cell wall-targeting domain of the S-layer protein EA1 and tetanus toxin fragment C (ToxC). This construct was expressed under the control of the promoter of the S-layer component gene. The hybrid protein was stably expressed on the cell surface of the bacterium. Mice were immunized with bacilli of the corresponding strain, and the hybrid protein elicited a humoral response to ToxC. This immune response was sufficient to protect mice against tetanus toxin challenge. Thus, the strategy developed in this study may make it possible to generate multivalent live veterinary vaccines, using the S-layer protein genes as a cell surface display system.

Ligand fishing based on cell surface display of enzymes for inhibitor screening

2021 ◽  
pp. 338359
Author(s):  
Yun-Cong Yuan ◽  
Xiao-Lin Bai ◽  
Yi-Ming Liu ◽  
Xiao-Yue Tang ◽  
Hao Yuan ◽  
...  
Keyword(s):  
Cell Surface ◽  
Surface Display ◽  
Cell Surface Display ◽  
Inhibitor Screening ◽  
Ligand Fishing

scholarly journals Cover Feature: Bacterial Cell‐Surface Display of Semisynthetic Cyclic Peptides (ChemBioChem 1/2019)

ChemBioChem ◽  
2018 ◽  
Vol 20 (1) ◽  
pp. 4-4
Author(s):  
Shubhendu Palei ◽  
Kira S. Becher ◽  
Christian Nienberg ◽  
Joachim Jose ◽  
Henning D. Mootz
Keyword(s):  
Cell Surface ◽  
Bacterial Cell ◽  
Cyclic Peptides ◽  
Surface Display ◽  
Cell Surface Display ◽  
Bacterial Cell Surface

scholarly journals Mammalian cell surface display for monoclonal antibody-based FACS selection of viral envelope proteins

mAbs ◽  
2017 ◽  
Vol 9 (7) ◽  
pp. 1052-1064 ◽  
Author(s):  
Tim-Henrik Bruun ◽  
Veronika Grassmann ◽  
Benjamin Zimmer ◽  
Benedikt Asbach ◽  
David Peterhoff ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Cell Surface ◽  
Mammalian Cell ◽  
Surface Display ◽  
Cell Surface Display ◽  
Envelope Proteins ◽  
Viral Envelope ◽  
Selection Of ◽  
Viral Envelope Proteins

Cell-surface display technology and metabolic engineering of Saccharomyces cerevisiae for enhancing xylitol production from woody biomass

2019 ◽  
Vol 21 (7) ◽  
pp. 1795-1808 ◽  
Author(s):  
Gregory Guirimand ◽  
Kentaro Inokuma ◽  
Takahiro Bamba ◽  
Mami Matsuda ◽  
Kenta Morita ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Metabolic Engineering ◽  
Cell Surface ◽  
Surface Display ◽  
Woody Biomass ◽  
Cell Surface Display ◽  
Xylitol Production ◽  
Display Technology ◽  
Pharmaceutical Industries

Xylitol is a major commodity chemical widely used in both the food and pharmaceutical industries.

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