Dietary exposure to methyl mercury chloride induces alterations in hematology, biochemical parameters, and mRNA expression of antioxidant enzymes and metallothionein in Nile tilapia

2021 ◽  
Author(s):  
Rasha T. M. Alam ◽  
Ehsan. H. Abu Zeid ◽  
Bouthaina A. Khalifa ◽  
Ahmed Hamed Arisha ◽  
Rasha M. Reda
Keyword(s):  
Antioxidant Enzymes ◽  
Mrna Expression ◽  
Methyl Mercury ◽  
Biochemical Parameters ◽  
Nile Tilapia ◽  
Dietary Exposure ◽  
Mercury Chloride

Neurobehavioral and immune-toxic impairments induced by organic methyl mercury dietary exposure in Nile tilapia Oreochromis niloticus

2021 ◽  
Vol 230 ◽  
pp. 105702
Author(s):  
Ehsan H. Abu Zeid ◽  
Bouthaina A. Khalifa ◽  
Enas N. Said ◽  
Ahmed H. Arisha ◽  
Rasha M. Reda
Keyword(s):  
Oreochromis Niloticus ◽  
Methyl Mercury ◽  
Nile Tilapia ◽  
Dietary Exposure ◽  
Nile Tilapia Oreochromis Niloticus

scholarly journals Aflatoxicosis Dysregulates the Physiological Responses to Crowding Densities in the Marine Teleost Gilthead Seabream (Sparus aurata)

Animals ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 753
Author(s):  
Andre Barany ◽  
Juan Fuentes ◽  
Gonzalo Martínez-Rodríguez ◽  
Juan Miguel Mancera
Keyword(s):  
Mrna Expression ◽  
Sparus Aurata ◽  
Stocking Density ◽  
Head Kidney ◽  
Dietary Exposure ◽  
Experimental Period ◽  
Gilthead Seabream ◽  
Marine Teleost ◽  
Lipid Metabolites ◽  
Period Duration

Several studies in fish have shown that aflatoxin B1 (AFB1) causes a disparity of species-dependent physiological disorders without compromising survival. We studied the effect of dietary administration of AFB1 (2 mg AFB1 kg−1 diet) in gilthead seabream (Sparus aurata) juveniles in combination with a challenge by stocking density (4 vs. 40 g L−1). The experimental period duration was ten days, and the diet with AFB1 was administered to the fish for 85 days prior to the stocking density challenge. Our results indicated an alteration in the carbohydrate and lipid metabolites mobilization in the AFB1 fed group, which was intensified at high stocking density (HSD). The CT group at HSD increased plasma cortisol levels, as expected, whereas the AFB1-HSD group did not. The star mRNA expression, an enzyme involved in cortisol synthesis in the head kidney, presented a ninefold increase in the AFB1 group at low stocking density (LSD) compared to the CT-LSD group. Adenohypophyseal gh mRNA expression increased in the AFB1-HSD but not in the CT-HSD group. Overall, these results confirmed that chronic AFB1 dietary exposure alters the adequate endocrinological physiological cascade response in S. aurata, compromising the expected stress response to an additional stressor, such as overcrowding.

Two cinnamate derivatives produce similar alteration in mRNA expression and activity of antioxidant enzymes in rats

2003 ◽  
Vol 17 (5) ◽  
pp. 255-262 ◽  
Author(s):  
Mi-Kyung Lee ◽  
Eun-Mi Park ◽  
Song-Hae Bok ◽  
Un Ju Jung ◽  
Ji-Young Kim ◽  
...  
Keyword(s):  
Antioxidant Enzymes ◽  
Mrna Expression ◽  
Expression And Activity

The effect of quercetin on the mRNA expression of different antioxidant enzymes in hepatoma cells

2003 ◽  
Vol 77 (9) ◽  
pp. 506-510 ◽  
Author(s):  
Elke R�hrdanz ◽  
Achim Bittner ◽  
Quynh-Hoa Tran-Thi ◽  
Regine Kahl
Keyword(s):  
Antioxidant Enzymes ◽  
Mrna Expression ◽  
Hepatoma Cells

Challenge with fumonisins B1 and B2 changes IGF-1 and GHR mRNA expression in liver of Nile tilapia fingerlings

2018 ◽  
Vol 11 (2) ◽  
pp. 237-245 ◽  
Author(s):  
S.C. Claudino-Silva ◽  
B. Lala ◽  
N.H.A.P. Mora ◽  
C.R. Schamber ◽  
C.S. Nascimento ◽  
...  
Keyword(s):  
Weight Gain ◽  
Mrna Expression ◽  
Feed Intake ◽  
Oreochromis Niloticus ◽  
Feed Efficiency ◽  
Nile Tilapia ◽  
Growth Hormone Receptor ◽  
Control Treatment ◽  
Insulin Growth Factor ◽  
Nile Tilapia Oreochromis Niloticus

Although fumonisins are identified as responsible for alterations in weight gain, little information is available on their effects on expression of growth-related genes, especially for Nile tilapia (Oreochromis niloticus) fingerlings. In this study, Nile tilapia fingerlings were treated with increasing levels of fumonisin B1 (FB1) and fumonisin B2 (FB2) (diets of 0, 20, 40, and 60 mg/kg) to evaluate their effects on weight gain (WG), feed intake (FI), feed efficiency (FE), growth hormone receptor (GHR) and insulin growth factor 1 (IGF-1) mRNA expression in liver of this fish. All variables were evaluated at 15 and 30 days of treatment. Diet containing 0 mg fumonisin/kg was used as control treatment. Treatment with 20, 40, and 60 mg fumonisin/kg of diet significantly reduced WG (P<0.0001) and FE (P<0.0001), while GHR and IGF-1 mRNA expression was reduced both at 15 and 30 days of treatment. Feed intake was not affected by diets in any of the evaluated periods. These results indicate that fumonisins (FB1 + FB2) affect the growth of Nile tilapia fingerlings through mechanisms that involve reduction of GHR and IGF-1 expression.

scholarly journals Muscle Antioxidant Enzymes Activity and Gene Expression Are Altered by Diet-Induced Increase in Muscle Essential Fatty Acid (α-linolenic acid) Concentration in Sheep Used as a Model

Nutrients ◽  
2019 ◽  
Vol 11 (4) ◽  
pp. 723 ◽  
Author(s):  
Eric Ponnampalam ◽  
Vahid Vahedi ◽  
Khageswor Giri ◽  
Paul Lewandowski ◽  
Joe Jacobs ◽  
...  
Keyword(s):  
Gene Expression ◽  
Antioxidant Enzymes ◽  
Fatty Acid ◽  
Mrna Expression ◽  
Linolenic Acid ◽  
Enzyme Activities ◽  
Antioxidant Enzyme Activities ◽  
Post Mortem ◽  
Annual Ryegrass ◽  
Alpha Linolenic Acid

This study investigated the effect of dietary manipulations on muscle fatty acid composition, the activities and relative mRNA expressions of antioxidant enzymes and the relationship between muscle enzyme activity or mRNA expression and alpha linolenic acid (ALA) concentration in sheep. Eighty-four lambs blocked on liveweight were randomly allocated to four dietary treatments, lucerne pasture (Lucerne), annual ryegrass pasture (Ryegrass), feedlot pellets (Feedlot) or annual ryegrass plus feedlot pellets (RyeFeedlot). After six weeks of feeding, lambs were slaughtered and within 30 min post-mortem, samples collected from the longissimus lumborum (LL) muscle for RNA isolation and measurement of antioxidant enzyme activities. At 24 h post-mortem, LL samples were collected for determination of fatty acid concentrations. Feedlot treatment decreased ALA, eicosapentaenoic (EPA), docosapentaenoic (DPA) and docosahexaenoic (DHA) concentrations compared with other treatments and increased linoleic acid (LA) and arachidonic acid (AA) compared with Lucerne and Ryegrass (p < 0.001). The activity of Glutathione peroxidase (GPX1, p < 0.001) and Superoxide dismutase (SOD2, p < 0.001) enzymes in the muscle increased with Lucerne compared to other treatments. Lucerne increased muscle gpx1 mRNA expression by 1.74-fold (p = 0.01) and 1.68-fold (p = 0.05) compared with Feedlot and other diets, respectively. The GPX1 (r2 = 0.319, p = 0.002) and SOD2 (r2 = 0.244, p = 0.009) enzyme activities were positively related to ALA. There was a positive linear relationship between muscle gpx1 (r2 = 0.102, p = 0.017) or sod2 (r2 = 0.049, p = 0.09) mRNA expressions and ALA concentration. This study demonstrates that diet can affect concentrations of ALA and other fatty acids as well as change activities and gene expression of antioxidant enzymes in muscle. Increased antioxidant activity may, in turn, have beneficial effects on the performance, health and wellbeing of animals and humans.

Sign in / Sign up

Export Citation Format

Share Document