Screening for Streptococcus pyogenes antibacterial and Candida albicans antifungal bioactivities of organic compounds in natural essential oils of Piper betle L., Cleistocalyx operculatus L. and Ageratum conyzoides L.

2020 ◽  
Author(s):  
Bui Thi Phuong Thuy ◽  
Le Trung Hieu ◽  
Tran Thi Ai My ◽  
Nguyen Thi Thanh Hai ◽  
Huynh Thi Phuong Loan ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Essential Oils ◽  
Organic Compounds ◽  
Streptococcus Pyogenes ◽  
Piper Betle ◽  
Ageratum Conyzoides ◽  
Cleistocalyx Operculatus

scholarly journals Antioxidant activity of some natural essential oils in Vietnam: Comparison between QSAR simulation and experimental study

2020 ◽  
Vol 129 (1D) ◽  
Author(s):  
Tran Thi Ai My ◽  
Le Trung Hieu ◽  
Nguyen Thi Thanh Hai ◽  
Ton Nu My Phuong ◽  
Huynh Thi Phuong Loan ◽  
...  
Keyword(s):  
Essential Oils ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Qsar Model ◽  
Piper Betle ◽  
Scavenging Activity ◽  
Dpph Radical ◽  
Ageratum Conyzoides ◽  
Dpph Radical Scavenging

Antioxidant activities of essential oils from leaves of Piper betle L. (T) and Cleistocalyx operculatus L. (V), and aerial parts of Ageratum conyzoides L. (H) natively grown in Thua Thien Hue province of Vietnam were investigated.Quantitative structure–activity relationship (QSAR) model was constructed from the 27 compounds including 4-hydroxy-chromene-2H-one and its derivatives. DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging activity was applied to estimate the antioxidant property of these essential oils through IC50 (mg/mL) values. QSAR model is used to predict the radical scavenging activity IC50 mg/mL of T, V, and H. The results indicate that there is a good agreement between the experimental data and the predicted values using the QSAR model. The three essential oils display the DPPH radical scavenging activities with the IC50 values being in the order of T > H > V of 3.71 mg/mL,596.44 mg/mL and 637.03 mg/mL, respectively. The essential oil of T exhibits the strong DPPH radical scavenging activity that is close to the reference compound ascorbic acid (IC50 value of 3.03 mg /mL).

Antifungal activity analysis of 3 essential oils (thyme, ajwainand orange) on candida albicans

2017 ◽  
Vol 6 (3) ◽  
pp. 2948-2950
Author(s):  
Vaishnavi Sivakali Subramanian ◽  
◽  
R.V Geetha ◽  
Anitha Roy ◽  
◽  
...  
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Essential Oils ◽  
Activity Analysis

scholarly journals Back to Nature: Combating Candida albicans Biofilm, Phospholipase and Hemolysin Using Plant Essential Oils

Antibiotics ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 81
Author(s):  
Ahmed M. El-Baz ◽  
Rasha A. Mosbah ◽  
Reham M. Goda ◽  
Basem Mansour ◽  
Taranum Sultana ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Essential Oils ◽  
Docking Study ◽  
New Drugs ◽  
Molecular Docking Study ◽  
Plant Essential Oils ◽  
Force Microscopy ◽  
Atomic Force ◽  
Afm Analysis ◽  
Phenotypic Methods

Candida albicans is the causative agent of fatal systemic candidiasis. Due to limitations of antifungals, new drugs are needed. The anti-virulence effect of plant essential oils (EOs) was evaluated against clinical C. albicans isolates including cinnamon, clove, jasmine and rosemary oils. Biofilm, phospholipase and hemolysin were assessed phenotypically. EOs were evaluated for their anti-virulence activity using phenotypic methods as well as scanning electron microscopy (SEM) and atomic force microscopy (AFM). Among the C. albicans isolates, biofilm, phospholipase and hemolysins were detected in 40.4, 86.5 and 78.8% of isolates, respectively. Jasmine oil showed the highest anti-biofilm activity followed by cinnamon, clove and rosemary oils. SEM and AFM analysis showed reduced adherence and roughness in the presence of EOs. For phospholipase, rosemary oil was the most inhibitory, followed by jasmine, cinnamon and clove oils, and for hemolysins, cinnamon had the highest inhibition followed by jasmine, rosemary and clove oils. A molecular docking study revealed major EO constituents as promising inhibitors of the Als3 adhesive protein, with the highest binding for eugenol, followed by 1,8-cineole, 2-phenylthiolane and cinnamaldehyde. In conclusion, EOs have a promising inhibitory impact on Candida biofilm, phospholipase and hemolysin production, hence EOs could be used as potential antifungals that impact virulence factors.

scholarly journals Candida albicans/Staphylococcus aureus Dual-Species Biofilm as a Target for the Combination of Essential Oils and Fluconazole or Mupirocin

2017 ◽  
Vol 182 (11-12) ◽  
pp. 989-995 ◽  
Author(s):  
Aleksandra Budzyńska ◽  
Sylwia Różalska ◽  
Beata Sadowska ◽  
Barbara Różalska
Keyword(s):  
Staphylococcus Aureus ◽  
Candida Albicans ◽  
Essential Oils

scholarly journals Analisa Aktivitas Antibakteri Rebusan Daun Sirih Dengan Rebusan Daun Kemangi Terhadap Pertumbuhan Bakteri Streptococcus mutans

2020 ◽  
Vol 18 (1) ◽  
pp. 36
Author(s):  
ENNY WILLIANTI ◽  
THEODORA THEODORA ◽  
WAHYUNI DYAH PARMASARI
Keyword(s):  
Antibacterial Activity ◽  
Essential Oils ◽  
Streptococcus Mutans ◽  
Observational Research ◽  
Ocimum Sanctum ◽  
Piper Betle ◽  
Oral Flora ◽  
Betel Leaf ◽  
Growth Of Bacteria

<p><strong>ABSTRACT</strong><strong></strong></p><p><strong> </strong></p><p><strong>Background</strong>: Betel leaf contains essential oils consisting of bethelphenol, kavikol, sesquiterpenes, hydroxycavikol, cavibetol, estragol, eugenol and carvacrol. Essential oils are antibacterial due to the presence of phenol compounds and their derivatives that can denature the bacterial cell proteins. Basil leaves contain compounds from essential oils, namely 1,8-cineole, ß-bisabolene, and methyl eugenol. These three ingredients are soluble to ethanol and can cause damage to the cell membranes of the Streptococcus mutans bacteria, which are members of the normal oral flora but can turn into pathogens if the balance of normal flora is disturbed. The aim of this study was to determine the difference in the activity of the antibacterial  of decoction betel leaf (piper betle L. ) with a decoction of basil leaves (ocimum sanctum) against growth of bacteria <em>Streptococcus mutans</em> (in vitro study).</p><p><strong>M</strong><strong>ethod:</strong> this observational research with disk diffusion techniques. This study observed and measured the diameter of the inhibitory zone in MHA formed by decoction of betel leaf (piper betle L) and basil leaf (ocimum sanctum) in units of millimeters (mm). There were 2 groups with 16 replications.</p><p><strong>R</strong><strong>esults</strong>: the results of the description test showed that the antibacterial activity of the betel leaf decoction and the highest decoction of basil leaf was 17 mm and the lowest was 15 mm, but the average antibacterial value of betel leaf decoction (15,81) greater than the average value of antibacterial activity of basil leaf (15.75). This is because there are chemicals contained in betel leaf similar as contained in basil leaf, namely essential oils.</p><p><strong>Conclusion</strong>: there is no difference in the antibacterial activity of decoction  betel leaf with decoction basil leaf against growth of bacteria <em>Streptococcus mutans</em>.</p><p><strong> </strong></p><p><strong>Keywords</strong>: Betel leaf decoction, basil leaf  decoction, Streptococcus <strong>mutans.      </strong></p><p><strong> </strong></p><p><strong> </strong></p><p><strong>Abstrak</strong><strong></strong></p><p><strong> </strong></p><p><strong>Latar Belakang</strong>: Daun sirih mengandung minyak atsiri yang terdiri dari <em>bethelphenol, kavikol, </em>seskuiterpen, hydroxycavikol,cavibetol, estragol, eugenol dan carvacrol. Minyak atsiri bersifat antibakteri karena adanya senyawa phenol dan turunannya yang dapat mendenaturasi protein sel bakteri. Daun kemangi mengandung senyawa dari minyak atsiri yaitu <em>1,8-cineole</em>, <em>ß-bisabolene</em>, <em>metyl eugenol</em>. Ketiga bahan tersebut memiliki sifat larut terhadap etanol dan dapat menyebabkan kerusakan membran sel bakteri <em>streptococcus mutans</em> yang merupakan anggota flora normal rongga mulut tetapi dapat berubah menjadi patogen jika keseimbangan flora normal terganggu.Tujuan penelitian ini untuk mengetahui perbedaan aktivitas antibakteri rebusan daun sirih (<em>piper betle</em> L) dengan rebusan daun kemangi (<em>ocimum sanctum</em>) terhadap pertumbuhan bakteri <em>Streptococcus mutans</em> (penelitian in vitro).</p><p><strong>Metode</strong>: penelitian observasional ini dengan teknik difusi. Penelitian ini dilakukan dengan mengamati dan mengukur diameter zona hambat pada MHA yang dibentuk oleh rebusan daun sirih (<em>piper betle</em> L) dan daun kemangi (<em>ocimum sanctum</em>) dalam satuan milimeter (mm). Terdapat 2 kelompok dengan replikasi sebanyak 16.</p><p><strong>Hasil</strong> : Hasil uji deskripsi menunjukkan bahwa aktivitas antibakteri pada rebusan daun sirih maupun rebusan daun kemangi yang tertinggi sebesar 17 mm dan yang terendah 15 mm. Tetapi pada nilai rata-rata efektifitas antibakteri rebusan daun sirih (15,81) lebih besar daripada nilai rata-rata efektifitas antibakteri rebusan daun kemangi (15,75). Hal ini dikarenakan ada zat kimia yang terkandung dalam daun sirih mirip dengan yang terkandung dalam daun kemangi, yaitu minyak atsiri.</p><p><strong>Kesimpulan</strong> : tidak ada perbedaan aktivitas antibakteri rebusan daun sirih dengan rebusan daun kemangi terhadap pertumbuhan bakteri <em>Streptococcus </em><em>m</em><em>utans</em>.</p><p><strong> </strong></p><p><strong>Kata kunci</strong>:  rebusan daun sirih, rebusan daun kemangi<em>, Streptococcus mutans</em>.</p><p> </p><p>     </p>

scholarly journals Antimicrobial Efficiency of Essential Oils from Traditional Medicinal Plants of Asir Region, Saudi Arabia over Drug Resistant Isolates

2018 ◽  
Author(s):  
Ismail M. Helal ◽  
Ashraf El-Bessoumy ◽  
Erwah Al-Bataineh ◽  
Martin R.P. Joseph ◽  
Parassena Rajagopalan ◽  
...  
Keyword(s):  
Saudi Arabia ◽  
Essential Oils ◽  
Streptococcus Pyogenes ◽  
Biological Activities ◽  
Ocimum Basilicum ◽  
Antimicrobial Efficacy ◽  
Chemical Compositions ◽  
Drug Resistant ◽  
Origanum Vulgare ◽  
Asir Region

Antimicrobial resistance (AMR) is a recurring global problem, which constantly demands new antimicrobial compounds to challenge the resistance. It is well known that essential oils (EOs) have been known for biological activities including antimicrobial properties. In this study, EOs from seven aromatic plants of Asir region of southwestern Saudi Arabia were tested for their antimicrobial efficacy against four drug resistant pathogenic bacterial isolates (Staphylococcus aureus, Streptococcus pyogenes, Escherichia coli and Streptococcus typhimurium) and one fungal isolate (Candida albicans). Chemical compositions of EOs were determined by Gas chromatography-Mass Spectrometry (GC-MS). The results revealed that EOs from Mentha cervina, Ocimum basilicum and Origanum vulgare proved most active against all isolates with inhibitory zone range between17 to 45 mm.&nbsp; The lowest minimum inhibitory concentration (MIC) of 0.025mg/ml was observed for Staph. aureus and Streptococcus pyogenes with EO of Origanum vulgare. All the three EOs showed significant anti candida activity. Together form the results the EOs from Mentha cervina, Ocimum basilicum and Origanum vulgare demonstrated a significant antimicrobial efficacy against drug resistant microorganisms.

scholarly journals VARIATIONS IN ANTIMICROBIAL ACTIVITY OF SYZYGIUM CUMINI OF DIFFERENT GEOGRAPHICAL LOCATIONS

2020 ◽  
pp. 1-6
Author(s):  
Pratibha ◽  
Nesari Tanuja ◽  
Ghildiyal Shivani ◽  
Vandhana
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Streptococcus Pyogenes ◽  
Antimicrobial Agents ◽  
Diffusion Method ◽  
Klebsiella Pneumonia ◽  
Syzygium Cumini ◽  
Geographical Variations ◽  
Geographical Locations

The emergence of antibiotic resistance and the evolution of new strains of disease causing agents, are of highly concern to the global health community. Plants are potential source of antimicrobial agents. They have been used traditionally for prevention of infections caused by micro-organisms. Description of Krimighana herbs enumerated in Ayurveda classics is suggestive towards the importance of this group of medicine. Jambu (Syzygium cumini (L.) Skeels) is a member of Myrtaceae family. In Raja Nighantu it is mentioned that plant Jambu is having Kriminashaka property. It has been widely used medicine in the prevention of various ailments like cough, Dysentary, Diabetes, inflammation and ringworm. It is well established fact that geographical variations effects the potential and activity of medicinal herbs. Hence, the present study was undertaken to investigate Syzygium cumini procured from different geographical locations including Delhi, Rajasthan and Maharashtra for their potential activity against human infections caused by pathogens. Method The aqueous extract of Syzygium cumini of all the three areas was prepared. The activity of the plant extract was evaluated against nine bacterial pathogens and one fungal strain, which include Staphyllococcus aureus, Streptococcus pyogenes, Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi, Klebsiella pneumonia and Candida albicans. The activity was carried out using Disk diffusion method. Result and Conclusion: All samples of Syzygium cumini showed potential antimicrobial activity against four pathogens including Staphyllococcus aureus, Streptococcus pyogenes, Pseudomonas aeruginosa and Candida albicans. MIC was also evaluated against the tested pathogenic strains. The sample from Maharashtra showed MIC i.e. 80µg, 40µg, 80µg against Staphyllococcus aureus, Streptococcus pyogenes and Candida albicans respectively which is less as compare to sample from Rajasthan and Delhi. Region wise sample from Maharashtra showed good ZOI and MIC.

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