Intracellular Changes of Metal Elements by Fucoidan Extracted from Brown Seaweed (Cladosiphon okamuranus)

This study was undertaken to elucidate physiological properties of fucoidan extracted from Cladosiphon okamuranus TOKITA (C. okamuranus) and Fucus vesiculosus (F.vesilurosus). According to the data on MTT assay and TUNEL staining, F. vesiculosus possessed more anti-proliferate activity than C. okamuranus through apoptosis induction. We determined the metal contents of fucoidan to evaluate the relevance of metal elements in its biological effects. Al , Fe and Zn levels were significantly increased in F. vesiculosus than in C. okamuranus. The intracellular changes of metal elements following the administration of fucoidan was analyzed by in-air micro-PIXE in TRL1215 cells (normal rat liver cell line). At 24 h after 5-bromo-2'-deoxyuridine ( BrdU ) dosing, TRL1215 cells were treated with 1.0 mg/mL of each fucoidan for 9 h. The X-ray spectra showed that F. vesiculosus-treated cells markedly increased the yield of aluminum ( Al ) compared to those of C. okamuranus -treated cells and the control. In F. vesiculosus -treated cells, the focal accumulation of Br was spatially correlated with Al map, suggesting that Al was localized within the nucleus. These findings suggest that F. vesiculosus fucoidan increases the accumulations of Al in rat hepatocytes, which may participate in anti-proliferate activity of this fucoidan.

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Antithrombotic Effect of Oral Administration of Mozuku (Cladosiphon okamuranus, Brown Seaweed) Extract in Rat

Journal of Nutritional Science and Vitaminology ◽

10.3177/jnsv.65.171 ◽

2019 ◽

Vol 65 (2) ◽

pp. 171-176 ◽

Cited By ~ 1

Author(s):

Toshinori YASUZAWA ◽

Akira MIMA ◽

Shigeru UESHIMA

Keyword(s):

Oral Administration ◽

Brown Seaweed ◽

Seaweed Extract ◽

Antithrombotic Effect ◽

Cladosiphon Okamuranus

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FUKOSANTIN: KAROTENOID BERHARGA DARI MAKROALGA COKLAT

OSEANA ◽

10.14203/oseana.2018.vol.43no.3.60 ◽

2018 ◽

Vol 43 (3) ◽

pp. 16-28

Author(s):

Tri Handayani

Keyword(s):

Undaria Pinnatifida ◽

Brown Seaweed ◽

Laminaria Japonica ◽

Brown Macroalgae ◽

Padina Tetrastromatica ◽

Sargassum Fulvellum ◽

Myagropsis Myagroides ◽

Cladosiphon Okamuranus ◽

Kjellmaniella Crassifolia ◽

Petalonia Binghamiae

FUCOXANTHIN: A HIGH VALUE CAROTENOID FROM BROWN MACROALGAE. Fucoxanthin is carotenoid that can be found in marine brown seaweed (macroalgae) and diatoms (microalgae). Fucoxanthin has been isolated from brown macroalgae for its bioactivities study, including: Cystoceira barbata, Sargassum aquifolium, Sargassum filipendula, Sargassum ilicifolium, Sargassum wightii, Alaria crassifolia, Cladosiphon okamuranus, Cystoseira hakodatensis, Eisenia bicyclis, Fucus serratus, Fucus vesiculosus, Hijikia fusiformis, Ishige okamurae, Kjellmaniella crassifolia, Laminaria japonica, Laminaria ochotensis, Myagropsis myagroides, Padina tetrastromatica, Petalonia binghamiae, Sargassum fulvellum, Sargassum heterophyllum, Sargassum horneri, Sargassum siliquastrum, Undaria pinnatifida, Sargassum longifolium, Padina sp, Turbinaria sp, and Undaria pinnatifida. The amount of fucoxanthin depends on the species of algae. Fucoxanthin has been reported to have bioactivities, i.e. antioxidant, antiinflammatory, anticancer, antiobese, antidiabetic, antiangiogenic, and pigmentation inhibitory.

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Intestinal Absorption of Fucoidan Extracted from the Brown Seaweed, Cladosiphon okamuranus

Marine Drugs ◽

10.3390/md13010048 ◽

2014 ◽

Vol 13 (1) ◽

pp. 48-64 ◽

Cited By ~ 54

Author(s):

Takeaki Nagamine ◽

Kyoumi Nakazato ◽

Satoru Tomioka ◽

Masahiko Iha ◽

Katsuyuki Nakajima

Keyword(s):

Intestinal Absorption ◽

Brown Seaweed ◽

Cladosiphon Okamuranus

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Morphometric Analysis of the Hepatocytes from Fish Exposed to Arsenic

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100091391 ◽

1976 ◽

Vol 34 ◽

pp. 282-283

Author(s):

Elsie M. B. Sorensen

Keyword(s):

Morphometric Analysis ◽

Arsenic Concentration ◽

Ultrastructural Changes ◽

Sodium Arsenate ◽

Lepomis Cyanellus ◽

Mammalian Counterpart ◽

Intracellular Changes ◽

Green Sunfish ◽

Organic Pesticides

The detoxification capacity of the liver is well documented for a variety of substances including ethanol, organic pesticides, drugs, and metals. The piscean liver, although less enzymatically active than the mammalian counterpart (1), contains endoplasmic reticulum with an impressive repertoire of oxidizing, reducing, and conjugating abilities (2). Histopathologic changes are kncwn to occur in fish hepatocytes following in vivo exposure to arsenic (3); however, ultrastructural changes have not been reported. This study involved the morphometric analysis of intracellular changes in fish parynchymal hepatocytes and correlation with arsenic concentration in the liver.Green sunfish (Lepomis cyanellus, R.) were exposed to 0, 30, or 60 ppm arsenic (as sodium arsenate) at 20°C for 1, 2, or 3 week intervals before removal of livers for quantification of the arsenic burden (using neutron activation analysis) and morphometric analysis of ultrastructural alterations. Livers were cut into 1 mm cubes for fixation, dehydration, and embedding.

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Ultrastructural changes in isolated rat liver perfused with cyclic heptapeptide toxins from norwegian freshwater cyanobacteria (blue-green algae)

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100128572 ◽

1987 ◽

Vol 45 ◽

pp. 858-859

Author(s):

A.S. Dabholkar ◽

W.W. Carmichael ◽

K. Berg ◽

J. Wyman

Keyword(s):

Ultrastructural Changes ◽

Sprague Dawley ◽

Isolated Rat Liver ◽

Blue Green Algae ◽

Sprague Dawley Rats ◽

Cyclic Heptapeptide ◽

Intracellular Changes ◽

Oscillatoria Agardhii ◽

Rat Livers ◽

Isolated Rat

Intracellular changes in the hepatocytes of isolated rat livers perfused with cyclic heptapeptide toxins are described. The toxins used are 1) -Ala-Leu- β-methyl isoAsp-Arg-ADDA-isoGlu-mdha (M.W. 944) from Microcystis aeruginosa- Lake Akersvatn, Norway; 2) -Ala-Arg-isoAsp-Arg-ADDA-isoGlu-mdha (M.W. 1023) from Oscillatoria agardhii var. - Lake Kolbatnvatn, Norway; 3) -Ala-Arg-isoAsp-Arg-ADDA-isoGlu-dha (M.W. 1009) from Oscillatoria agardhii var. isothrix - Lake Froylandsvatn, Norway. Approximate LD intraperitoneal mouse for the toxins is 50, 500 and 1000 μg/kg respectively.Livers were removed from male Sprague Dawley rats and perfused for 15 min with a blood-free perfusate (50 ml) followed by 60 min with perfusate containing i) 25, 50, or 200 μg of M. aeruginosa toxin ii) 50, 250, 500 or 1000 μg of O. agardhii var. toxin and iii) 1000, 2000, 2500 or 5000 μg of O. agardhii var. isothrix toxin. Control livers were perfused for 75 min with the blood-free perfusate.

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Modification of Si/Mg Composite Supported Ziegler- Natta Ti-based Catalysts for Propylene Polymerization Using the Third Metal Elements

JOURNAL OF POLYMER MATERIALS ◽

10.32381/jpm.2018.35.03.2 ◽

2019 ◽

Vol 35 (3) ◽

pp. 265-279

Author(s):

WEI ZHU ◽

◽

ZHOU TIAN ◽

PINGKAI JIANG ◽

BOPING LIU ◽

...

Keyword(s):

Propylene Polymerization ◽

Metal Elements ◽

The Third

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Decreasing water pressure cues ‘bailout’ in seaweed-associated crustaceans

Marine Ecology Progress Series ◽

10.3354/meps13485 ◽

2020 ◽

Vol 653 ◽

pp. 121-129

Author(s):

RB Taylor ◽

S Patke

Keyword(s):

Water Pressure ◽

Rapid Change ◽

Brown Seaweed ◽

Model System ◽

Rate Of Change ◽

Suitable Habitat ◽

Changing Light ◽

Surface Increase ◽

Reduced Water ◽

Transparent Chamber

Small mobile crustaceans are abundant on seaweeds. Many of these crustaceans rapidly abandon their host if it is detached from the seafloor and floats towards the surface, but the trigger for this ‘bailout’ behaviour is unknown. We tested 2 potential cues, i.e. rapid change in light and rapid change in water pressure, using >1 mm epifauna on the brown seaweed Carpophyllum plumosum as a model system. Bailout occurred in response to reduced water pressure, but not to changing light, as (1) bailout occurred at similar rates in light and dark, (2) bailout occurred on the seafloor when water pressure was reduced within a transparent chamber by the equivalent of ~0.5 m depth or more, and (3) little bailout occurred when water pressure was held constant within the chamber while seaweeds were raised to the surface. Increase in pressure (simulating sinking) did not induce bailout. The rate of bailout increased with increasing magnitude of pressure reduction but was not influenced greatly by the rate of change of pressure within the range tested (up to an equivalent of 0.4 m depth s-1). The use of pressure rather than light as a cue for bailout is consistent with the need for seaweed-associated crustaceans to rapidly abandon a detached host and relocate to suitable habitat during both day and night.

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