scholarly journals 1H, 15 N and 13C resonance assignments of the C-terminal domain of PulL, a component of the Klebsiella oxytoca type II secretion system

2021 ◽  
Author(s):  
Régine Dazzoni ◽  
Aracelys López-Castilla ◽  
Florence Cordier ◽  
Benjamin Bardiaux ◽  
Michael Nilges ◽  
...  
Keyword(s):  
Klebsiella Oxytoca ◽  
Resonance Assignments ◽  
Secretion System ◽  
Type Ii Secretion ◽  
Type Ii ◽  
Terminal Domain ◽  
Type Ii Secretion System

scholarly journals Pilotin-secretin recognition in the type II secretion system of Klebsiella oxytoca

2011 ◽  
Vol 82 (6) ◽  
pp. 1422-1432 ◽  
Author(s):  
Tommaso Tosi ◽  
Nicholas N. Nickerson ◽  
Luca Mollica ◽  
Malene Ringkjøbing Jensen ◽  
Martin Blackledge ◽  
...  
Keyword(s):  
Klebsiella Oxytoca ◽  
Secretion System ◽  
Type Ii Secretion ◽  
Type Ii ◽  
Type Ii Secretion System

scholarly journals Signal Recognition Particle-Dependent Inner Membrane Targeting of the PulG Pseudopilin Component of a Type II Secretion System

2006 ◽  
Vol 189 (5) ◽  
pp. 1783-1793 ◽  
Author(s):  
Olivera Francetic ◽  
Nienke Buddelmeijer ◽  
Shawn Lewenza ◽  
Carol A. Kumamoto ◽  
Anthony P. Pugsley
Keyword(s):  
Signal Sequence ◽  
Klebsiella Oxytoca ◽  
Signal Recognition Particle ◽  
Secretion System ◽  
Membrane Targeting ◽  
Type Ii Secretion ◽  
Type Ii ◽  
Signal Recognition ◽  
Inner Membrane ◽  
Type Ii Secretion System

ABSTRACT The pseudopilin PulG is an essential component of the pullulanase-specific type II secretion system from Klebsiella oxytoca. PulG is the major subunit of a short, thin-filament pseudopilus, which presumably elongates and retracts in the periplasm, acting as a dynamic piston to promote pullulanase secretion. It has a signal sequence-like N-terminal segment that, according to studies with green and red fluorescent protein chimeras, anchors unassembled PulG in the inner membrane. We analyzed the early steps of PulG inner membrane targeting and insertion in Escherichia coli derivatives defective in different protein targeting and export factors. The β-galactosidase activity in strains producing a PulG-LacZ hybrid protein increased substantially when the dsbA, dsbB, or all sec genes tested except secB were compromised by mutations. To facilitate analysis of native PulG membrane insertion, a leader peptidase cleavage site was engineered downstream from the N-terminal transmembrane segment (PrePulG*). Unprocessed PrePulG* was detected in strains carrying mutations in secA, secY, secE, and secD genes, including some novel alleles of secY and secD. Furthermore, depletion of the Ffh component of the signal recognition particle (SRP) completely abolished PrePulG* processing, without affecting the Sec-dependent export of periplasmic MalE and RbsB proteins. Thus, PulG is cotranslationally targeted to the inner membrane Sec translocase by SRP.

scholarly journals Solution Structure of Homology Region (HR) Domain of Type II Secretion System

2012 ◽  
Vol 287 (12) ◽  
pp. 9072-9080 ◽  
Author(s):  
Shuang Gu ◽  
Geoff Kelly ◽  
Xiaohui Wang ◽  
Tom Frenkiel ◽  
Vladimir E. Shevchik ◽  
...  
Keyword(s):  
Solution Structure ◽  
Secretion System ◽  
Type Ii Secretion ◽  
Type Ii ◽  
Homology Region ◽  
Type Ii Secretion System

scholarly journals Generation of Xylose-inducible promoter tools for Pseudomonas species and their use in implicating a role for the Type II secretion system protein XcpQ in inhibition of corneal epithelial wound closure

2020 ◽  
Author(s):  
Jake D. Callaghan ◽  
Nicholas A. Stella ◽  
Kara M. Lehner ◽  
Benjamin R. Treat ◽  
Kimberly M. Brothers ◽  
...  
Keyword(s):  
Gene Expression ◽  
Wound Closure ◽  
Inducible Promoter ◽  
Secretion System ◽  
Type Ii Secretion ◽  
Type Ii ◽  
Corneal Epithelial ◽  
E Coli ◽  
Type Ii Secretion System ◽  
Promoter System

ABSTRACTTunable control of gene expression is an invaluable tool for biological experiments. In this study, we describe a new xylose-inducible promoter system and evaluate it in both Pseudomonas aeruginosa and P. fluorescens. The Pxut promoter derived from the P. flurorescens xut operon was incorporated into a broad host-range pBBR1-based plasmid and compared to the Escherichia coli-derived PBAD promoter using gfp as a reporter. GFP-fluorescence from the Pxut promoter was inducible in both Pseudomonas species, but not in E. coli, which may facilitate cloning of toxic genes using E. coli to generate plasmids. The Pxut promoter was expressed at a lower inducer concentration than PBAD in P. fluorescens and higher gfp levels were achieved using Pxut. Flow cytometry analysis indicated that Pxut was more leaky than PBAD in the tested Pseudomonas species, but was expressed in a higher proportion of cells when induced. D-xylose did not support growth of P. aeruginosa or P. fluorescens as a sole carbon source and is less expensive than many other commonly used inducers which could facilitate large scale applications. The efficacy of this system aided in demonstrating a role for the P. aeruginosa type II secretion system gene from xcpQ in bacterial inhibition of corneal epithelial cell wound closure. This study introduces a new inducible promoter system for gene expression for use in Pseudomonas species.ImportancePseudomonas species are enormously important in human infections, biotechnology, and as a model system for interrogating basic science questions. In this study we have developed a xylose-inducible promoter system and evaluated it in P. aeruginosa and P. fluorescens and found it to be suitable for the strong induction of gene expression. Furthermore, we have demonstrated its efficacy in controlled gene expression to show that a type 2 secretion system protein from P. aeruginosa, XcpQ, is important for host-pathogen interactions in a corneal wound closure model.

scholarly journals Structural and Functional Studies on the Interaction of GspC and GspD in the Type II Secretion System

2011 ◽  
Vol 7 (9) ◽  
pp. e1002228 ◽  
Author(s):  
Konstantin V. Korotkov ◽  
Tanya L. Johnson ◽  
Michael G. Jobling ◽  
Jonathan Pruneda ◽  
Els Pardon ◽  
...  
Keyword(s):  
Secretion System ◽  
Type Ii Secretion ◽  
Type Ii ◽  
Functional Studies ◽  
Type Ii Secretion System
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