scholarly journals Development of A MERS-CoV Replicon Cell Line for Antiviral Screening

2021 ◽  
Author(s):  
Jing Chen ◽  
Bing-Jie Hu ◽  
Kai Zhao ◽  
Yun Luo ◽  
Hao-Feng Lin ◽  
...  
Keyword(s):  
Cell Line ◽  
Replicon Cell ◽  
Replicon Cell Line

Colony Forming Assay for HCV-Replicon Cell Line

BIO-PROTOCOL ◽  
2013 ◽  
Vol 3 (24) ◽  
Author(s):  
Chang Lee ◽  
Seong-Wook Lee
Keyword(s):  
Cell Line ◽  
Replicon Cell ◽  
Replicon Cell Line ◽  
Colony Forming Assay

scholarly journals Inhibitors of Alphavirus Entry and Replication Identified with a Stable Chikungunya Replicon Cell Line and Virus-Based Assays

PLoS ONE ◽  
2011 ◽  
Vol 6 (12) ◽  
pp. e28923 ◽  
Author(s):  
Leena Pohjala ◽  
Age Utt ◽  
Margus Varjak ◽  
Aleksei Lulla ◽  
Andres Merits ◽  
...  
Keyword(s):  
Cell Line ◽  
Replicon Cell ◽  
Replicon Cell Line ◽  
Alphavirus Entry

Development of a stable Japanese encephalitis virus replicon cell line for antiviral screening

2017 ◽  
Vol 162 (11) ◽  
pp. 3417-3423 ◽  
Author(s):  
Qiu-Yan Zhang ◽  
Xiao-Dan Li ◽  
Si-Qing Liu ◽  
Cheng-Lin Deng ◽  
Bo Zhang ◽  
...  
Keyword(s):  
Japanese Encephalitis Virus ◽  
Cell Line ◽  
Japanese Encephalitis ◽  
Encephalitis Virus ◽  
Replicon Cell ◽  
Replicon Cell Line

scholarly journals Derivation of a novel SARS–coronavirus replicon cell line and its application for anti-SARS drug screening

Virology ◽  
2007 ◽  
Vol 360 (1) ◽  
pp. 150-158 ◽  
Author(s):  
Feng Ge ◽  
Yonghu Luo ◽  
Pei Xiong Liew ◽  
Eugene Hung
Keyword(s):  
Cell Line ◽  
Drug Screening ◽  
Sars Coronavirus ◽  
Replicon Cell ◽  
Replicon Cell Line

scholarly journals Identification and Evaluation of Coronavirus Replicase Inhibitors Using a Replicon Cell Line

2006 ◽  
pp. 609-613 ◽  
Author(s):  
Elke Scandella ◽  
Klara K. Eriksson ◽  
Tobias Hertzig ◽  
Christian Drosten ◽  
Lili Chen ◽  
...  
Keyword(s):  
Cell Line ◽  
Replicon Cell ◽  
Replicon Cell Line

Identification of Alphavirus Inhibitors by Using Virus-Based Assays and a Chikungunya Replicon Cell Line

2011 ◽  
Vol 90 (2) ◽  
pp. A52-A53
Author(s):  
Tero Ahola ◽  
Leena Pohjala ◽  
Pasi Kaukinen ◽  
Age Utt ◽  
Margus Varjak ◽  
...  
Keyword(s):  
Cell Line ◽  
Replicon Cell ◽  
Replicon Cell Line

Development of a replicon cell line-based high throughput antiviral assay for screening inhibitors of Zika virus

2018 ◽  
Vol 150 ◽  
pp. 148-154 ◽  
Author(s):  
Jia-Qi Li ◽  
Cheng-Lin Deng ◽  
Dayong Gu ◽  
Xiao Li ◽  
Lei Shi ◽  
...  
Keyword(s):  
Cell Line ◽  
High Throughput ◽  
Zika Virus ◽  
Replicon Cell ◽  
Replicon Cell Line

scholarly journals Cellular RNA Helicase p68 Relocalization and Interaction with the Hepatitis C Virus (HCV) NS5B Protein and the Potential Role of p68 in HCV RNA Replication

2004 ◽  
Vol 78 (10) ◽  
pp. 5288-5298 ◽  
Author(s):  
Phuay-Yee Goh ◽  
Yee-Joo Tan ◽  
Siew Pheng Lim ◽  
Y. H. Tan ◽  
Seng Gee Lim ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Cell Line ◽  
Rna Helicase ◽  
Hcv Rna ◽  
Activity Levels ◽  
Nonstructural Proteins ◽  
Negative Strand ◽  
Replicon Cell Line ◽  
Rna Helicase P68

ABSTRACT Chronic infection by hepatitis C virus (HCV) can lead to severe hepatitis and cirrhosis and is closely associated with hepatocellular carcinoma. The replication cycle of HCV is poorly understood but is likely to involve interaction with host factors. In this report, we show that NS5B, the HCV RNA-dependent RNA polymerase (RdRp), interacts with a human RNA helicase, p68. Transient expression of NS5B alone, as well as the stable expression of all the nonstructural proteins in a HCV replicon-bearing cell line (V. Lohmann, F. Korner, J.-O. Koch, U. Herian, L. Theilmann, and R. Bartenschlager, Science 285:110-113), causes the redistribution of endogenous p68 from the nucleus to the cytoplasm. Deletion of the C-terminal two-thirds of NS5B (NS5BΔC) dramatically reduces its coimmunoprecipitation (co-IP) with endogenous p68, while the deletion of the N-terminal region (NS5BΔN1 and NS5BΔN2) does not affect its interaction with p68. In consistency with the co-IP results, NS5BΔC does not cause the relocalization of p68 whereas NS5BΔN1 does. With a replicon cell line, we were not able to detect a change in positive- and negative-strand synthesis when p68 levels were reduced using small interfering RNA (siRNA). In cells transiently transfected with a full-length HCV construct, however, the depletion (using specific p68 siRNA) of endogenous p68 correlated with a reduction in the transcription of negative-strand from positive-strand HCV RNA. Overexpression of NS5B and NS5BΔN1, but not that of NS5BΔC, causes a reduction in the negative-strand synthesis, indicating that overexpressed NS5B and NS5BΔN1 sequesters p68 from the replication complexes (thus reducing their replication activity levels). Identification of p68 as a cellular factor involved in HCV replication, at least for cells transiently transfected with a HCV expression construct, is a step towards understanding HCV replication.

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