Signaling pathways underlying changes in the contractility of the stomach fundus smooth muscle in diabetic rats

2020 ◽  
Vol 43 (6) ◽  
pp. 666-675
Author(s):  
Dong Min Kim ◽  
Tin Myo Khing ◽  
Wynn Thein ◽  
Won Seok Choi ◽  
Chang Yell Shin ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Signaling Pathways ◽  
Diabetic Rats ◽  
Stomach Fundus

Phenotype modulation in primary cultures of aortic smooth muscle cells from streptozotocin-diabetic rats

1998 ◽  
Vol 63 (4) ◽  
pp. 225-236 ◽  
Author(s):  
Pascale Etienne ◽  
Núria Parés-Herbuté ◽  
Louis Monnier ◽  
Herisoa Rabesandratana ◽  
Laurence Mani-Ponset ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Primary Cultures ◽  
Muscle Cells ◽  
Diabetic Rats ◽  
Aortic Smooth Muscle Cells ◽  
Aortic Smooth Muscle ◽  
Streptozotocin Diabetic Rats

scholarly journals Adenosine Attenuates Human Coronary Artery Smooth Muscle Cell Proliferation by Inhibiting Multiple Signaling Pathways That Converge on Cyclin D

Hypertension ◽  
2015 ◽  
Vol 66 (6) ◽  
pp. 1207-1219 ◽  
Author(s):  
Raghvendra K. Dubey ◽  
Jürgen Fingerle ◽  
Delbert G. Gillespie ◽  
Zaichuan Mi ◽  
Marinella Rosselli ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Smooth Muscle ◽  
Coronary Artery ◽  
Smooth Muscle Cell ◽  
Signaling Pathways ◽  
Muscle Cell ◽  
Smooth Muscle Cell Proliferation ◽  
Cyclin D ◽  
Human Coronary Artery ◽  
Multiple Signaling

scholarly journals Microarray analysis reveals novel gene expression changes associated with erectile dysfunction in diabetic rats

2005 ◽  
Vol 23 (2) ◽  
pp. 192-205 ◽  
Author(s):  
Chris J. Sullivan ◽  
Thomas H. Teal ◽  
Ian P. Luttrell ◽  
Khoa B. Tran ◽  
Mette A. Peters ◽  
...  
Keyword(s):  
Gene Expression ◽  
Smooth Muscle ◽  
Erectile Dysfunction ◽  
Splice Variants ◽  
Full Range ◽  
Diabetic Rats ◽  
Differentially Expressed ◽  
Diabetic Rat ◽  
Literature Mining ◽  
Mrna Levels

To investigate the full range of molecular changes associated with erectile dysfunction (ED) in Type 1 diabetes, we examined alterations in penile gene expression in streptozotocin-induced diabetic rats and littermate controls. With the use of Affymetrix GeneChip arrays and statistical filtering, 529 genes/transcripts were considered to be differentially expressed in the diabetic rat cavernosum compared with control. Gene Ontology (GO) classification indicated that there was a decrease in numerous extracellular matrix genes (e.g., collagen and elastin related) and an increase in oxidative stress-associated genes in the diabetic rat cavernosum. In addition, PubMatrix literature mining identified differentially expressed genes previously shown to mediate vascular dysfunction [e.g., ceruloplasmin ( Cp), lipoprotein lipase, and Cd36] as well as genes involved in the modulation of the smooth muscle phenotype (e.g., Kruppel-like factor 5 and chemokine C-X3-C motif ligand 1). Real-time PCR was used to confirm changes in expression for 23 relevant genes. Further validation of Cp expression in the diabetic rat cavernosum demonstrated increased mRNA levels of the secreted and anchored splice variants of Cp. CP protein levels showed a 1.9-fold increase in tissues from diabetic rats versus controls. Immunohistochemistry demonstrated localization of CP protein in cavernosal sinusoids of control and diabetic animals, including endothelial and smooth muscle layers. Overall, this study broadens the scope of candidate genes and pathways that may be relevant to the pathophysiology of diabetes-induced ED as well as highlights the potential complexity of this disorder.

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