scholarly journals Screening Sugarcane Wild Accessions for Resistance to Sugarcane Mosaic Virus (SCMV)

Sugar Tech ◽  
2014 ◽  
Vol 17 (3) ◽  
pp. 252-257 ◽  
Author(s):  
M. F. Silva ◽  
M. C. Gonçalves ◽  
M. N. G. Melloni ◽  
D. Perecin ◽  
M. G. A. Landell ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Sugarcane Mosaic Virus ◽  
Wild Accessions

scholarly journals Maize resistance to Sugarcane mosaic virus

2017 ◽  
Vol 38 (SI 2 - 6th Conf EFPP 2002) ◽  
pp. 542-544
Author(s):  
R. Pokorný ◽  
M. Porubová
Keyword(s):  
Mosaic Virus ◽  
Systemic Infection ◽  
Sugarcane Mosaic Virus ◽  
Resistant Line ◽  
Mechanisms Of Resistance ◽  
Long Distance ◽  
Maize Hybrids ◽  
Resistant Lines ◽  
Maize Resistance ◽  
Long Distance Movement

Under greenhouse conditions 12 maize hybrids derived from crosses of four resistant lines with several lines of different level of susceptibility were evaluated for resistance to Czech isolate of Sugarcane mosaic virus (SCMV). These hybrids were not fully resistant to isolate of SCMV, but the symptoms on their newly growing leaves usually developed 1 to 3 weeks later in comparison with particular susceptible line, the course of infection was significantly slower and rate of infection lower. As for mechanisms of resistance, the presence of SCMV was detected by ELISA in inoculated leaves both of resistant and susceptible lines, but virus was detected 7 days later in resistant line. Systemic infection developed only in susceptible lines. These results indicate restriction of viral long distance movement in the resistant line.

scholarly journals Variabilidade genética de Sugarcane mosaic virus, causando mosaico em milho no Brasil

2011 ◽  
Vol 46 (4) ◽  
pp. 362-369 ◽  
Author(s):  
Marcos Cesar Gonçalves ◽  
Diogo Manzano Galdeano ◽  
Ivan de Godoy Maia ◽  
César Martins Chagas
Keyword(s):  
Mosaic Virus ◽  
Sao Paulo ◽  
Sugarcane Mosaic Virus ◽  
Maize Dwarf Mosaic Virus ◽  
São Paulo ◽  
Rt Pcr ◽  
Das Elisa

O objetivo deste trabalho foi caracterizar biológica e molecularmente três isolados de Sugarcane mosaic virus (SCMV) de lavouras de milho, analisá-los filogeneticamente e discriminar polimorfismos do genoma. Plantas com sintomas de mosaico e nanismo foram coletadas em lavouras de milho, no Estado de São Paulo e no Município de Rio Verde, GO, e seus extratos foliares foram inoculados em plantas indicadoras e submetidos à análise sorológica com antissoros contra o SCMV, contra o Maize dwarf mosaic virus (MDMV) e contra o Johnsongrass mosaic virus (JGMV). Mudas de sorgo 'Rio' e 'TX 2786' apresentaram sintomas de mosaico após a inoculação dos três isolados, e o DAS-ELISA confirmou a infecção pelo SCMV. O RNA total foi extraído e usado para amplificação por transcriptase reversa seguida de reação em cadeia de polimerase (RT-PCR). Fragmentos específicos foram amplificados, submetidos à análise por polimorfismo de comprimento de fragmento de restrição (RFLP) e sequenciados. Foi possível discriminar os genótipos de SCMV isolados de milho de outros isolados brasileiros do vírus. Alinhamentos múltiplos e análises dos perfis filogenéticos corroboram esses dados e mostram diversidade nas sequências de nucleotídeos que codificam para a proteína capsidial, o que explica o agrupamento separado desses isolados e sugere sua classificação como estirpes distintas, em lugar de simples isolados geográficos.

scholarly journals Identification, Occurrence, and Distribution of Strains Sugarcane Mosaic in Puerto Rico

1969 ◽  
Vol 38 (4) ◽  
pp. 188-198
Author(s):  
G. W. Bruehl
Keyword(s):  
Puerto Rico ◽  
Mosaic Virus ◽  
Strain Distribution ◽  
Field Strain ◽  
Sugarcane Mosaic Virus ◽  
Mechanical Transmission ◽  
Geographic Pattern ◽  
Major Strain ◽  
Field Reaction

Three strains of sugarcane mosaic, A, B, and D, described in Louisiana, were found to make up the bulk of the collections of the sugarcane mosaic virus in Puerto Rico. Strain B was most prevalent. It was the major strain found on B.H. 10(12), B. 34104, and M. 336. Strain D was equally abundant on B. 37161. B. 37161 showed some, resistance to mosaic in the field as compared with B. 34104. This may be due to some resistance to strain B. If B. 37161 is not kept free of mosaic, the preferred strain D may increase in it and render it more susceptible in the field. No marked geographic pattern of strain distribution was observed. Mechanical transmission of mosaic by the Sein method gave a better correlation with field reaction of varieties than did transmission by the Matz method. Strain A was most easily transmitted mechanically, while in the field strain B predominated in most varieties. Therefore, the vectors of Puerto Rico may be more efficient transmitters of strain B than of A.

scholarly journals Exploración de la interacción entre la región 5´UTR del Sugarcane Mosaic Virus (SCMV) y proteínas del hospedero maíz

Respuestas ◽  
2017 ◽  
Vol 22 (1) ◽  
pp. 103
Author(s):  
Giovanni Chaves-Bedoya
Keyword(s):  
Mosaic Virus ◽  
Protein Complex ◽  
Sugarcane Mosaic Virus ◽  
Host Proteins ◽  
T7 Promoter ◽  
Viral Genomes ◽  
Uv Crosslinking ◽  
Maize Leaves ◽  
The Stability ◽  
Replicative Cycle

Resumen Antecedentes: El virus del mosaico de la caña de azúcar, SCMV (Potyviridae) es un miembro de la gran familia de virus de ARN de cadena positiva, sin una estructura CAP en su extremo 5´no traducido (5`UTR), pero con una proteína viral unida al genoma (VPg) y una cola poli A en su extremo 3´UTR. Se ha sugerido que proteínas del hospedero hacen un puente entre las regiones no traducidas virales 5´y 3´ para potenciar la traducción viral. Dado que las regiones no traducidas presentes en los genomas virales contienen elementos involucrados en la regulación de su ciclo replicativo, es importante analizar la interacción entre estas regiones y las proteínas virales o del hospedero para inferir su función. Objetivo: Determinar si existen proteínas del hospedero maíz que pudieran estar interactuando con la región 5´UTR de SCMV. Metodología: La región 5´UTR de SCMV se amplificó por PCR incluyendo la secuencia del promotor T7 en la secuencia del oligo 5´ UTR y se generaron sus transcritos marcados radiactivamente. Los transcritos marcados fueron entrecruzados con extractos proteicos de hojas de maíz sanas e infectadas. Resultados: Los resultados sugieren la presencia de una proteína putativa de interacción del hospedante maíz de aproximadamente 53kDa, con la región 5´UTR de SCMV. Conclusión: Los ensayos de entrecruzamiento, especificidad y estabilidad de los complejos ARN-proteína sugieren que en el hospedante maíz existe al menos una proteína que interactúa con la región 5´ UTR de SCMV.Palabras Clave: Complejo ARN-proteína, entrecruzamiento UV, fracción proteica, Potyvirus, VPg.Abstract Background: Sugarcane mosaic virus is a member of the great family of positive sense RNA viruses, without a CAP structure in its 5´UTR end, but with a viral protein attached to the genome (VPg) and a poli A tail in its 3´UTR end. It has been suggested that some host proteins make a bridge between the untranslated 5´and 3´ regions (UTRs) to enhance the canonical and/or non-canonical virus translation. Since the UTR regions present in the viral genomes have some elements involved in the regulation of their replicative cycle, it is important to analyze the interaction that may occur between these regions and the viral or host proteins to infer regarding its function. Objective: To identify proteins of the host maize that could be interacting with the 5´UTR region of SCMV. Methodology: 5´UTR region of SCMV was amplified by PCR including the sequence of the T7 promoter in the 5´oligo sequence. The transcripts were radioactively labeled. Labeled transcripts were crosslinked with proteins extracts from healthy and infected maize leaves. Results: The results suggest the presence of a 53 kDa putative protein interacting with the 5 ´UTR region of SCMV. Conclusion: Crosslinking essays, specificity and the stability of the RNA-protein complex suggest that in maize host there is at least one protein that interacts with the 5´UTR region of SCMV.Keywords: RNA-Protein complex, UV crosslinking, protein fraction, Potyvirus, VPg

scholarly journals Characterization of a highly divergent Sugarcane mosaic virus from Canna indica L. by deep sequencing

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Yongqiang Li ◽  
Fei Xia ◽  
Yixuan Wang ◽  
Chenge Yan ◽  
Anning Jia ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Deep Sequencing ◽  
Viral Disease ◽  
Mosaic Disease ◽  
Sugarcane Mosaic Virus ◽  
Full Genome Sequence ◽  
Mosaic Symptom ◽  
Cdna Clones ◽  
Pathogenicity Test ◽  
Sequence Identities

Abstract Background Cannas are popular ornamental plants and widely planted for the beautiful foliage and flower. Viral disease is a major threaten to canna horticulture industry. In the city of Beijing, mosaic disease in canna was frequently observed, but the associated causal agent and its biological characterization is still unknown. Results After small RNA deep sequencing, 36,776 contigs were assembled and 16 of them shared high sequence identities with the different proteins of Sugarcane mosaic virus (SCMV) of the size ranging from 86 to 1911 nt. The complete genome of SCMV isolate (canna) was reconstructed by sequencing all cDNA clones obtained from RT-PCR and 5′\3′ RACE amplifications. SCMV-canna isolate showed to have a full RNA genome of 9579 nt in length and to share 78% nt and 85% aa sequence identities with SCMV isolates from other hosts. The phylogenetic tree constructed based on the full genome sequence of SCMV isolates allocated separately the canna-isolate in a distinct clade, indicating a new strain. Recombination analyses demonstrated that SCMV-canna isolate was a recombinant originating from a sugarcane-infecting isolate (major parent, acc. no. AJ310103) and a maize-infecting isolate (minor parent, acc. no. AJ297628). Pathogenicity test showed SCMV-canna could cause typical symptoms of mosaic and necrosis in some tested plants with varying levels of severity but was less virulent than the isolate SCMV-BJ. Field survey showed that the virus was widely distributed. Conclusions This study identified SCMV as the major agent causing the prevalent mosaic symptom in canna plants in Beijing and its genomic and biological characterizations were further explored. All these data enriched the knowledge of the viruses infecting canna and would be helpful in effective disease management in canna.

Sign in / Sign up

Export Citation Format

Share Document