scholarly journals Preparation and characterization of sericin powder extracted with deep sea water

3 Biotech ◽  
2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Sun Mee Hong ◽  
Sung Chang Choi ◽  
Hyun Mee Park ◽  
Young Sik Seok
Keyword(s):  
Deep Sea ◽  
2010 ◽  
Vol 56 (5) ◽  
pp. 421-431 ◽  
Author(s):  
C. William Yeung ◽  
Kenneth Lee ◽  
Lyle G. Whyte ◽  
Charles W. Greer

The Gully is the first Fisheries and Oceans Canada marine protected area off the eastern coast of Canada. To ensure success of conservation efforts in this area, it is essential to develop a better understanding of microbial community composition from the euphotic zone to the deep sea in this previously unsurveyed environment. Denaturing gradient gel electrophoresis (DGGE) and nucleotide sequencing were used to characterize microbial community structure. DGGE results showed a clear difference in the microbial community structure between the euphotic zone and the deep sea water. Cluster analysis showed high similarity (>85%) for all the samples taken from below 500 m, but lower similarity (49%–72%) when comparing samples from above and below 500 m. Changes in microbial community structure with depth corresponded well with changes in oceanographic physical parameters. Furthermore, 16S rRNA gene analysis showed that the bacterioplankton sequences generally clustered into 1 of 9 major lineages commonly found in marine systems. However, not all the major lineages were detected at all the different depths. The SAR11 and SAR116 sequences were only present in the surface water, and the SAR324 and Actinobacteria sequences were only present in deep sea water. These findings provide a preliminary characterization of the microbial communities of this unique ecosystem.


2019 ◽  
Vol 69 (4) ◽  
pp. 932-936 ◽  
Author(s):  
Qiliang Lai ◽  
Xiupian Liu ◽  
Jun Yuan ◽  
Shuchen Xie ◽  
Zongze Shao

A taxonomic study was carried out on strain CIC4N-9T, which was isolated from deep-sea water of the Indian Ocean. The bacterium was Gram-stain-negative, catalase- and oxidase-positive, rod-shaped and non-motile. Growth was observed at salinities of 0–9% and at temperatures of 4–41 °C. The isolate was able to degrade gelatin but not aesculin. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain CIC4N-9T belonged to the genus Pararhodobacter , with the highest sequence similarity to the only recognized species, Pararhodobacter aggregans D1-19T (96.9 %). The average nucleotide identity and estimated DNA–DNA hybridization values between strain CIC4N-9T and P. aggregans D1-19T were 80.4 and 23.0 %, respectively. The principal fatty acids were summed feature 8 (C18 : 1 ω7c and/or C18 : 1 ω6c), C16 : 0, C18 : 1ω7c 11-methyl, C18 : 0 and C17 : 0. The G+C content of the chromosomal DNA was 66.8 mol%. The sole respiratory quinone was determined to be Q-10. Phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, two unknown phospholipids, four unknown aminolipids and one unknown polar lipid were present. The combined genotypic and phenotypic data show that strain CIC4N-9T represents a novel species within the genus Pararhodobacter , for which the name Pararhodobacter marinus sp. nov. is proposed. The type strain is CIC4N-9T (=MCCC 1A01225T=KCTC 52336T).


2020 ◽  
Vol 10 (15) ◽  
pp. 5183
Author(s):  
Jain Nam ◽  
Kyeong Jin Kim ◽  
Geonhee Park ◽  
Byeong Goo Kim ◽  
Gwi-Hwa Jeong ◽  
...  

This study aimed to determine the effect of deep-sea water (DSW)-derived mineral waters on intestinal health, using a cell model and a dextran sulfate sodium (DSS)-induced enteritis mouse model. DSW was desalted and minerals were added to generate mineral waters that were classified as trace mineral (TM), high magnesium (HM), high magnesium low salt (HMLS), and high magnesium high calcium (HMHC), using a tabletop electrodialysis device. Caco-2 cells cocultured with Raw264.7 cells were either pre-treated or not with the four water groups, and inflammation was induced by treatment with lipopolysaccharide (LPS). Compared to LPS-treated Caco-2 cells, HMLS-cotreated cells maintained high transepithelial electrical resistance, similar to control cells. FITC-dextran permeability was lower in HMLS-treated than in other cells. In vivo, in comparison to DSS-treated mice, colon shortening was inhibited, and disease activity and colon injury were suppressed in HMLS-cotreated mice. RNA-seq of colonic tissues revealed that inflammatory gene expression was similar among the control and HMLS mice, and DSS-induced expression of inflammation-related genes such as TNF-α and NOS2 and inflammatory chemokine genes was suppressed. Our findings suggest that DSW-derived mineral water intake can help reduce colitis symptoms, and the effects may be partially regulated by magnesium and other minerals.


Author(s):  
Bounmy Keohavong ◽  
Jun Yeob Lee ◽  
Jeong Heon Lee ◽  
Sung Ki Lee ◽  
Gur Yoo Kim ◽  
...  

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