Immunotoxicity of metal oxide nanoparticle: zinc oxide

2014 ◽  
Vol 10 (3) ◽  
pp. 237-244 ◽  
Author(s):  
Rosa Mistica C. Ignacio ◽  
Cheol-Su Kim ◽  
Soo-Ki Kim
Keyword(s):  
Zinc Oxide ◽  
Metal Oxide ◽  
Metal Oxide Nanoparticle ◽  
Oxide Nanoparticle

scholarly journals Comparative Molecular Immunological Activity of Physiological Metal Oxide Nanoparticle and its Anticancer Peptide and RNA Complexes

Nanomaterials ◽  
2019 ◽  
Vol 9 (12) ◽  
pp. 1670 ◽  
Author(s):  
Robert K. DeLong ◽  
Jeffrey Comer ◽  
Elza Neelima Mathew ◽  
Majid Jaberi-Douraki
Keyword(s):  
Zinc Oxide ◽  
Metal Oxide ◽  
Metastatic Melanoma ◽  
Poly I:C ◽  
Lung Cells ◽  
Immunological Activity ◽  
Anticancer Peptide ◽  
Metal Oxide Nanoparticle ◽  
Physiologically Based ◽  
Oxide Nanoparticle

Currently, there is a great interest in nanoparticle-based vaccine delivery. Recent studies suggest that nanoparticles when introduced into the biological milieu are not simply passive carriers but may also contribute immunological activity themselves or of their own accord. For example there is considerable interest in the biomedical applications of one of the physiologically-based inorganic metal oxide nanoparticle, zinc oxide (ZnO). Indeed zinc oxide (ZnO) NP are now recognized as a nanoscale chemotherapeutic or anticancer nanoparticle (ANP) and several recent reports suggest ZnO NP and/or its complexes with drug and RNA induce a potent antitumor response in immuno-competent mouse models. A variety of cell culture studies have shown that ZnO NP can induce cytokines such as IFN-γ, TNF-α, IL-2, and IL-12 which are known to regulate the tumor microenvironment. Much less work has been done on magnesium oxide (MgO), cobalt oxide (Co3O4), or nickel oxide (NiO); however, despite the fact that these physiologically-based metal oxide NP are reported to functionally load and assemble RNA and protein onto their surface and may thus also be of potential interest as nanovaccine platform. Here we initially compared in vitro immunogenicity of ZnO and Co3O4 NP and their effects on cancer-associated or tolerogenic cytokines. Based on these data we moved ZnO NP forward to testing in the ex vivo splenocyte assay relative to MgO and NiO NP and these data showed significant difference for flow cytometry sorted population for ZnO-NP, relative to NiO and MgO. These data suggesting both molecular and cellular immunogenic activity, a double-stranded anticancer RNA (ACR), polyinosinic:poly cytidylic acid (poly I:C) known to bind ZnO NP; when ZnO-poly I:C was injected into B16F10-BALB/C tumor significantly induced, IL-2 and IL-12 as shown by Cohen’s d test. LL37 is an anticancer peptide (ACP) currently in clinical trials as an intratumoral immuno-therapeutic agent against metastatic melanoma. LL37 is known to bind poly I:C where it is thought to compete for receptor binding on the surface of some immune cells, metastatic melanoma and lung cells. Molecular dynamic simulations revealed association of LL37 onto ZnO NP confirmed by gel shift assay. Thus using the well-characterized model human lung cancer model cell line (BEAS-2B), poly I:C RNA, LL37 peptide, or LL37-poly I:C complexes were loaded onto ZnO NP and delivered to BEAS-2B lung cells, and the effect on the main cancer regulating cytokine, IL-6 determined by ELISA. Surprisingly ZnO-LL37, but not ZnO-poly I:C or the more novel tricomplex (ZnO-LL37-poly I:C) significantly suppressed IL-6 by >98–99%. These data support the further evaluation of physiological metal oxide compositions, so-called physiometacomposite (PMC) materials and their formulation with anticancer peptide (ACP) and/or anticancer RNA (ACR) as a potential new class of immuno-therapeutic against melanoma and potentially lung carcinoma or other cancers.

An Assessment of Fluorescence- and Absorbance-Based Assays to Study Metal-Oxide Nanoparticle ROS Production and Effects on Bacterial Membranes

Small ◽  
2012 ◽  
Vol 9 (9-10) ◽  
pp. 1753-1764 ◽  
Author(s):  
Allison M. Horst ◽  
Raja Vukanti ◽  
John H. Priester ◽  
Patricia A. Holden
Keyword(s):  
Metal Oxide ◽  
Ros Production ◽  
Bacterial Membranes ◽  
Metal Oxide Nanoparticle ◽  
Oxide Nanoparticle

Metal oxide nanoparticle-functionalized sebacic acid-grafted PHEAM nanocarriers for enriched activity of metronidazole against food borne bacteria: in vitro and in vivo study

2018 ◽  
Vol 42 (22) ◽  
pp. 18437-18447 ◽  
Author(s):  
Murugesan Gowri ◽  
Kannan Suganya ◽  
Nachimuthu Latha ◽  
Marudhamuthu Murugan ◽  
Mukhtar Ahmed ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Metal Oxide ◽  
Sebacic Acid ◽  
In Vivo Study ◽  
Metal Oxide Nanoparticle ◽  
Food Borne ◽  
Oxide Nanoparticle

Food borne infection is a serious complication caused by Listeria monocytogenes (L. monocytogenes), a dangerous bacteria.

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