scholarly journals Critical role for the long non-coding RNA AFAP1-AS1 in the proliferation and metastasis of hepatocellular carcinoma

Tumor Biology ◽  
2016 ◽  
Vol 37 (7) ◽  
pp. 9699-9707 ◽  
Author(s):  
Xu Lu ◽  
Chuang Zhou ◽  
Renfeng Li ◽  
Zhiwei Liang ◽  
Wenlong Zhai ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Critical Role ◽  
Non Coding Rna ◽  
Proliferation And Metastasis ◽  
Long Non Coding Rna

Long non-coding RNA THOR promotes cell proliferation and metastasis in hepatocellular carcinoma

Gene ◽  
2018 ◽  
Vol 678 ◽  
pp. 129-136 ◽  
Author(s):  
Zhangjun Cheng ◽  
Zhengqing Lei ◽  
Pinghua Yang ◽  
Anfeng Si ◽  
Daimin Xiang ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Cell Proliferation ◽  
Non Coding Rna ◽  
Proliferation And Metastasis ◽  
Long Non Coding Rna ◽  
Cell Proliferation And Metastasis

Long non-coding RNA ZEB1-AS1 promotes proliferation and metastasis of hepatocellular carcinoma cells by targeting miR-299-3p/E2F1 axis

2021 ◽  
Author(s):  
Baiyin Mu ◽  
Chenlan Lv ◽  
Qingli Liu ◽  
Hong Yang
Keyword(s):  
Hepatocellular Carcinoma ◽  
Cell Proliferation ◽  
Luciferase Reporter ◽  
Migration And Invasion ◽  
Non Coding Rna ◽  
Tumorigenesis And Progression ◽  
Novel Biomarkers ◽  
Proliferation And Metastasis ◽  
Long Non Coding Rna

Abstract There is emerging evidence that dysregulation of long non-coding RNAs (lncRNAs) is associated with hepatocellular carcinoma (HCC). Zinc finger E-box binding homeobox 1 antisense 1 (ZEB1-AS1) functions as an oncogenic regulator in various malignancies. Nonetheless, the potential role of ZEB1-AS1 in HCC remains poorly elucidated. Herein, qRT-PCR was employed for examining ZEB1-AS1, miR-299-3p and E2F1 mRNA expressions in HCC cells and tissues. MTT assay was performed to evaluate cell proliferation. Transwell assay was utilized for evaluating cancer cell migration and invasion. Western blot was employed for measuring E2F1 protein expression. What’s more, dual-luciferase reporter assay was utilized for verifying the targeting relationships between ZEB1-AS1 and miR-299-3p, as well as E2F1 and miR-299-3p. It was demonstrated that, in HCC tissues and cells, ZEB1-AS1 expression was markedly increased, and meanwhile, its high expression level is related to the unfavorable clinicopathologic indicators. ZEB1-AS1 overexpression facilitated HCC cell proliferation, migration and invasion, while its knockdown led to the opposite effects. In terms of mechanism, we discovered that ZEB1-AS1 could decoy miR-299-3p and up-regulate E2F1 expression. This work reveals the functions and mechanism of ZEB1-AS1 in HCC tumorigenesis and progression, which provides novel biomarkers and therapeutic targets for HCC.

scholarly journals A Novel Long Non-coding RNA, ENSG00000236199, Inhibits Proliferation and Metastasis through NRIP1 by Sponging miR-576-5p in Hepatocellular Carcinoma

2020 ◽  
Author(s):  
Yifeng Cui ◽  
Han Lin ◽  
Yunzheng Zhao ◽  
Jiaming Liu ◽  
Chengpeng Zhang ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Malignant Tumors ◽  
Quantitative Polymerase Chain Reaction ◽  
Luciferase Reporter Assay ◽  
Luciferase Reporter ◽  
Reporter Assay ◽  
Non Coding Rna ◽  
Proliferation And Metastasis ◽  
Mesenchymal Transformation ◽  
Long Non Coding Rna

Abstract Background: Hepatocellular carcinoma (HCC) is a common malignancy of the digestive system. A novel long non-coding RNA, ENSG00000236199 (lncRNA-199) , whose role in tumors has not been reported, especially in HCC. Methods: The expression of lncRNA-199 and miR-576-5p were detected by Real-time quantitative polymerase chain reaction (RT-qPCR), NRIP1 was measured by Western blotting. HCC cell proliferation and metastasis of HCC were examined using functional tests. Luciferase reporter assay was performed to confirm the relationship between lncRNA-199 and miR-576-5p, between miR-576-5p and NRIP1. Results: LncRNA-199 was frequently down-regulated in HCC and this down-regulation was negatively correlated with prognosis. Overexpression of lncRNA-199 could inhibit the growth and metastasis of HCC, while knockdown lncRNA-199 had the opposite effect. Down-regulated lncRNA-199 also could induce epithelial-mesenchymal transformation (EMT). Luciferase reporter assay demonstrated lncRNA-199 could modulate NRIP1 by sponge miR-576-5p. Conclusion: LncRNA-199 inhibited growth and metastasis through miR-576-5p/NRIP1 axis in HCC. This study revealed a novel unknown lncRNA function in malignant tumors, especially in HCC. At the same time, the regulatory mechanism of lncRNA-199 in HCC was clarified.

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