QTL analysis of seed germination traits in tobacco (Nicotiana tabacum L.)

AbstractGenetic mapping of seed germination traits has been performed with many plant species. In tobacco, however, investigations are rare. In the present study, a bi-parental mapping population consisting of 118 doubled haploid lines and derived from a cross between ‘Beinhart-1000’ and ‘Hicks’ was investigated. Four germination-related traits, total germination (TG), normal germination (NG), time to reach 50% of total germination (T50), and the area under the curve after 200 h of germination (AUC) were considered by examining seeds either untreated or after a moderate controlled deterioration (CD). Quantitative trait loci were found for all traits distributed on 11 out of the 24 linkage groups. It was demonstrated that, as in many other species, germination-related traits are very complex and under polygenic control.

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Seed germination and seedling vigour of transgenic tobacco (Nicotiana tabacum L.) with increased proline accumulation under osmotic stress

The Journal of Horticultural Science and Biotechnology ◽

10.1080/14620316.2018.1499423 ◽

2018 ◽

Vol 94 (2) ◽

pp. 220-228 ◽

Cited By ~ 2

Author(s):

Fabiana de Lima Abrantes ◽

Alessandra Ferreira Ribas ◽

Luiz Gonzaga Esteves Vieira ◽

Nelson Barbosa Machado-Neto ◽

Ceci Castilho Custódio

Keyword(s):

Nicotiana Tabacum ◽

Seed Germination ◽

Osmotic Stress ◽

Transgenic Tobacco ◽

Proline Accumulation ◽

Seedling Vigour ◽

Nicotiana Tabacum L

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Genetic Control of Facultative Parthenocarpy in Nicotiana tabacum L.

Journal of Heredity ◽

10.1093/jhered/esz025 ◽

2019 ◽

Vol 110 (5) ◽

pp. 610-617

Author(s):

Abigail Dexter-Boone ◽

Matt Humphry ◽

Rui Shi ◽

Ramsey S Lewis

Keyword(s):

Nicotiana Tabacum ◽

Major Quantitative Trait Locus ◽

Mapping Population ◽

Genomic Region ◽

Nicotiana Tabacum L ◽

Seedless Fruits ◽

Tobacco Cultivar ◽

Trait Locus ◽

Fruit Formation ◽

Maternal Haploids

Abstract Investigation of parthenocarpy, the production of fruit without fertilization, in multiple plant species could result in development of technologies for conferring seedless fruits and increased stability of fruit formation in economically important plants. We studied parthenocarpy in the model species Nicotiana tabacum L., and observed variability for expression of the trait among diverse genetic materials. Parthenocarpy was found to be partially dominant, and a single major quantitative trait locus on linkage group 22 was found to control the trait in a doubled haploid mapping population derived from a cross between parthenocarpic cigar tobacco cultivar “Beinhart 1000” and nonparthenocarpic flue-cured tobacco cultivar, “Hicks.” The same genomic region was found to be involved with control of the trait in the important flue-cured tobacco cultivar, “K326.” We also investigated the potential for the production of maternal haploids due to parthenogenesis in parthenocarpic tobacco seed capsules. Maternal haploids were not observed in parthenocarpic capsules, suggesting a requirement of fertilization for maternal haploid production due to parthenogenesis in N. tabacum.

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EFFECTS OF DIETHYL SULPHATE ON SEED GERMINATION AND SEEDLING GROWTH OF NICOTIANA TABACUM

Canadian Journal of Genetics and Cytology ◽

10.1139/g69-009 ◽

1969 ◽

Vol 11 (1) ◽

pp. 72-76 ◽

Cited By ~ 1

Author(s):

Bronius Povilaitis

Keyword(s):

Nicotiana Tabacum ◽

Seed Germination ◽

Seedling Growth ◽

Detrimental Effect ◽

Saturated Solution ◽

Mutagenic Agent ◽

Nicotiana Tabacum L ◽

Treated Seed ◽

Plant Characteristics

Seeds of three flue-cured tobacco (Nicotiana tabacum L.) varieties, Hicks Broadleaf, Yellow Gold, and Canadel, were soaked for 1, 2, 3 or 5 hours in a saturated solution of diethyl sulphate, a chemical mutagenic agent, to increase variability of plant characteristics. Diethyl sulphate had a detrimental effect on both extent and rate of germination; each decreased with length of soaking period. Seedlings from treated seed grew more slowly than controls.

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Studies on the relationship between ploidy level, morphology, the concentration of some phytohormones and the nicotine concentration of haploid and doubled haploid tobacco (Nicotiana tabacum L.) and NICA plants

Primary and Secondary Metabolism of Plants and Cell Cultures III ◽

10.1007/978-94-011-0237-7_6 ◽

1994 ◽

pp. 135-141

Author(s):

B. Zeppernick ◽

F. Schäfer ◽

K. Paasch ◽

B. Arnholdt-Schmitt ◽

K.-H. Neumann

Keyword(s):

Nicotiana Tabacum ◽

Ploidy Level ◽

Doubled Haploid ◽

Nicotine Concentration ◽

Nicotiana Tabacum L ◽

The Relationship

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Vigor and variation expressed by anther-derived doubled haploids of burley tobacco (Nicotiana tabacum L.). I. Comparison of sexual and doubled-haploid populations

Euphytica ◽

10.1007/bf00028538 ◽

1986 ◽

Vol 35 (1) ◽

pp. 33-40 ◽

Cited By ~ 8

Author(s):

W. R. Deaton ◽

G. B. Collins ◽

M. T. Nielsen

Keyword(s):

Nicotiana Tabacum ◽

Doubled Haploid ◽

Doubled Haploids ◽

Burley Tobacco ◽

Nicotiana Tabacum L

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Studies on the relationship between ploidy level, morphology, the concentration of some phytohormones and the nicotine concentration of haploid and doubled haploid tobacco (Nicotiana tabacum L.) and NICA plants

Plant Cell Tissue and Organ Culture (PCTOC) ◽

10.1007/bf00033870 ◽

1994 ◽

Vol 38 (2-3) ◽

pp. 135-141

Author(s):

B. Zeppernick ◽

F. Sch�fer ◽

K. Paasch ◽

B. Arnholdt-Schmitt ◽

K. H. Neumann

Keyword(s):

Nicotiana Tabacum ◽

Ploidy Level ◽

Doubled Haploid ◽

Nicotine Concentration ◽

Nicotiana Tabacum L ◽

The Relationship

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Phytochrome B regulates seed germination by integrating light and temperature signals in Nicotiana tabacum L.

10.21203/rs.2.16311/v1 ◽

2019 ◽

Author(s):

Zhenhua Li ◽

Xianya Wang ◽

Yiling Liu ◽

Min Zhang

Keyword(s):

Nicotiana Tabacum ◽

Seed Germination ◽

Low Temperature ◽

Light Exposure ◽

Continuous Light ◽

Light Signal ◽

Phytochrome B ◽

Nicotiana Tabacum L ◽

Significant Difference ◽

Light Darkness

Abstract Background: Phytochrome is the most abundant photoreceptor in Arabidopsis thaliana ( Arabidopsis ), which integrates light and temperature signals, and in turn regulates plant development. However, the exact pattern of integrated signals during seed germination remains unknown. In this study, we analyzed the effect of NtPHYB1 genotype in response to ecological environments in Nicotiana tabacum L. Results: The germination frequencies of WT seeds showed at least no significant difference, and were significantly higher than that of NtPHYB1 - GFP and NtPHYB1 -RNAi seeds in some environments or. According to the maximum germination frequency , germination of NtPHYB1 - GFP seeds was mainly inhibited by continuous light exposure, while the germination of NtPHYB1- RNAi seeds was repressed by low temperature and no light (darkness) exposure. At 15ºC, the germinations of all three genotypic seeds were inhibited by the low-temperature, and the germination frequency of NtPHYB1 - GFP seeds was significantly lower than that of WT and NtPHYB1 -RNAi seeds; while light signal had no effect at 15ºC. At 20 and 25ºC, the temperature signal promoted germination, and the signal of light was dispensable. At this condition, the maximum germination frequencies were obtained for NtPHYB1 - GFP and WT seeds. At 30 and 35ºC, the light signal was indispensable to maintain seed germination for all three genotypic seeds. At this condition, NtPHYB1 -RNAi seeds reached the maximum germination frequency. Conclusion: Phytochrome B regulates seed germination by integrating light and temperature signals. The above results elucidate why warm spring and autumn (about 25ºC) are more suitable for sowing compared to cool winters (less than 15ºC) and hot summers (greater than 30ºC).

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