Development of immunodiagnostics for Apple stem pitting virus and Apple mosaic virus infecting apple in India

2021 ◽  
Author(s):  
Sunny Dhir ◽  
Vijay Lakshmi ◽  
Vipin Hallan
Keyword(s):  
Mosaic Virus ◽  
Apple Stem ◽  
Apple Stem Pitting Virus ◽  
Stem Pitting

scholarly journals Gospodarski važni virusi jezgričavog voća

2019 ◽  
Vol 22 (3-4) ◽  
pp. 123-136
Author(s):  
Dario Ivić
Keyword(s):  
Mosaic Virus ◽  
Leaf Spot ◽  
Spot Virus ◽  
Apple Stem Grooving Virus ◽  
Apple Stem ◽  
Chlorotic Leaf ◽  
Apple Stem Pitting Virus ◽  
Stem Pitting

Virusi jabuke, kruške ili dunje relativno su slabo poznati stručnjacima i voćarima. Najvažnijim virusima koji se javljaju na jezgričavim voćnim vrstama smatraju se virus mozaika jabuke (Apple mosaic virus, ApMV), virus klorotične pjegavosti lista jabuke (Apple chlorotic leaf spot virus, ACLSV), virus brazdavosti debla jabuke (Apple stem grooving virus, ASGV) i virus jamičavosti debla jabuke (Apple stem pitting virus,ASPV). U radu je ukratko opisana njihova važnost, biologija i regulativni status, kao i osnovne mjere zaštite.

scholarly journals Rapid and Sensitive Detection of Apple stem pitting virus in Apple Trees Through RNA Amplification and Probing with Fluorescent Molecular Beacons

2001 ◽  
Vol 91 (11) ◽  
pp. 1085-1091 ◽  
Author(s):  
M. M. Klerks ◽  
G. Leone ◽  
J. L. Lindner ◽  
C. D. Schoen ◽  
J. F. J. M. van den Heuvel
Keyword(s):  
Field Testing ◽  
Molecular Beacons ◽  
Fluorescent Detection ◽  
Rna Amplification ◽  
Apple Trees ◽  
Apple Stem ◽  
Bark Tissue ◽  
Specific Amplification ◽  
Apple Stem Pitting Virus ◽  
Stem Pitting

Currently, detection of Apple stem pitting virus (ASPV; genus Foveavirus) in apple trees for certification purposes occurs by woody indexing. This method requires a minimum of 12 to 24 weeks in greenhouse testing to up to 2 years in field testing. In this paper, the development of a single tube AmpliDet RNA system for the rapid gel-free detection of ASPV in apple tree tissues is described. The system relies on the specific amplification of the viral RNA by nucleic acid sequence-based amplification and the simultaneous fluorescent detection of the amplification product through molecular beacons. A sensitivity of a minimum of 100 molecules of transcript RNA was obtained by the ASPV-specific AmpliDet RNA. All biologically characterized ASPV isolates from a field trial and 12 of 14 isolates from a plant virus collection were readily detected with this AmpliDet RNA system. In addition, the efficiency of this method for detecting ASPV in ‘Golden Delicious’ and ‘Gravenstein’ apple trees was compared throughout the year with mechanical inoculation onto Nicotiana occidentalis 37B, a candidate indicator for ASPV. This revealed that only AmpliDet RNA consistently detected the virus in bark tissue, irrespective of the season. Season-specific tissues such as buds, petals, and fruits, but not leaves, also were reliable sources for detection of ASPV by the AmpliDet RNA system.

Optimization of tissue and time for rapid serological and molecular detection of Apple stem pitting virus and Apple stem grooving virus in apple’

2018 ◽  
Vol 46 (5) ◽  
pp. 705-713 ◽  
Author(s):  
Sajad Un Nabi ◽  
Javid Iqbal Mir ◽  
Om Chand Sharma ◽  
Desh Beer Singh ◽  
Shafia Zaffer ◽  
...  
Keyword(s):  
Molecular Detection ◽  
Apple Stem Grooving Virus ◽  
Apple Stem ◽  
Apple Stem Pitting Virus ◽  
Stem Pitting

scholarly journals The application of RT-PCR assay for the detection of Apple stem pitting virus and Apple stem grooving virus in four apple cultivars

2012 ◽  
Vol 38 (No. 1) ◽  
pp. 13-17 ◽  
Author(s):  
J.K. Kundu
Keyword(s):  
Mixed Infection ◽  
Rt Pcr ◽  
Pome Fruit ◽  
Chain Reaction ◽  
Pcr Assay ◽  
Apple Stem Grooving Virus ◽  
Apple Stem ◽  
Apple Cultivars ◽  
Apple Stem Pitting Virus ◽  
Stem Pitting

The reverse transcription polymerace chain reaction (RT-PCR) assay was successfully used for the detection of Apple stem pitting virus (ASPV) and Apple stem grooving virus (ASGV) in four apple cultivars of a 25 years old orchard. These two main pome fruit viruses were detected frequently in all tested apple cultivars. ASGV and ASPV occurred in as many as 16 trees (in the cultivar Spartan) and 13 trees (in the cultivar Idared) out of 20 tested trees, respectively. Mixed infection by ASGV and ASPV was found in all tested cultivars (as many as 9 out of 20 tested trees of the cultivar Spartan).

scholarly journals Sequence diversity and potential recombination events in the coat protein gene of Apple stem pitting virus

2011 ◽  
Vol 158 (1-2) ◽  
pp. 263-267 ◽  
Author(s):  
Beata Komorowska ◽  
Paweł Siedlecki ◽  
Szymon Kaczanowski ◽  
Beata Hasiów-Jaroszewska ◽  
Tadeusz Malinowski
Keyword(s):  
Coat Protein ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Sequence Diversity ◽  
Apple Stem ◽  
Apple Stem Pitting Virus ◽  
Stem Pitting
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